FastQCFastQC Report
Thu 26 May 2016
SRR1512108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174758
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67743.87621739777292No Hit
TATCAACGCAGAGTACTTTTTTTTT50292.877693725036908No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT47052.6922944872337746No Hit
GGTATCAACGCAGAGTACTTTTTTT45522.604744847160073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA43052.4634065393286715No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24411.3967887020908913No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23861.3653166092539397No Hit
GTACATAAGCAGTGGTATCAACGCA21821.2485837558223372No Hit
GGTATCAACGCAGAAAAAAAAAAAA16220.9281406287551929No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16090.9207017704482771No Hit
GCTTATGTACTCTGCGTTGATACCA10300.5893864658556404No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9750.5579143730186886No Hit
GGTATCAACGCAGAGAAAAAAAAAA9540.5458977557536708No Hit
GTATCAACGCAGAGAAAAAAAAAAA8150.4663591938566474No Hit
ATCAACGCAGAGTACTTTTTTTTTT8110.46407031437759644No Hit
GTATCAACGCAGAAAAAAAAAAAAA6990.39998168896416764No Hit
GTGGTATCAACGCAGAGTACTTTTT6020.3444763615971801No Hit
CTTATGTACTCTGCGTTGATACCAC5810.3324597443321622No Hit
GAGTACATAAGCAGTGGTATCAACG5420.3101431694114146No Hit
TATCAACGCAGAGAAAAAAAAAAAA5240.299843211755685No Hit
GTGGTATCAACGCAGAGTACATGGG4910.280959956053514No Hit
GGTATCAACGCAGAGTACATGGGGG4580.262076700351343No Hit
TATCAACGCAGAAAAAAAAAAAAAA4250.243193444649172No Hit
TATGTACTCTGCGTTGATACCACTG4180.2391879055608327No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA4150.2374712459515444No Hit
CATAAGCAGTGGTATCAACGCAGAG3800.21744355050984787No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3290.18826033715194726No Hit
CAACGCAGAGTACTTTTTTTTTTTT3190.18253813845431968No Hit
ACGCAGAGTACATAAGCAGTGGTAT3150.18024925897526864No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3140.1796770391055059No Hit
GTATCAACGCAGAGTACATAAGCAG3140.1796770391055059No Hit
GTACTGGTTCACTATCGGTCAGTCA3120.1785325993659804No Hit
GCGTTGATACCACTGCTTATGTACT3100.17738815962645488No Hit
TATCAACGCAGAGTACATAAGCAGT3010.17223818079859005No Hit
GGTATCAACGCAGAGTACATGGGGA2970.169949301319539No Hit
TCAACGCAGAGTACTTTTTTTTTTT2780.15907712379404662No Hit
AACGCAGAGTACTTTTTTTTTTTTT2680.15335492509641904No Hit
GGTATCAACGCAGAGTACATAAGCA2530.14477162704997767No Hit
CTGCTTATGTACTCTGCGTTGATAC2470.14133830783140114No Hit
GTGGTATCAACGCAGAAAAAAAAAA2160.12359949186875566No Hit
ACTCTGCGTTGATACCACTGCTTAT2070.11844951304089082No Hit
GTATCAACGCAGAGTACATGGGGGG2060.11787729317112808No Hit
AAGCAGTGGTATCAACGCAGAGTAC2010.11501619382231429No Hit
GTACATGGGGTGGTATCAACGCAGA1980.113299534213026No Hit
CCCATGTACTCTGCGTTGATACCAC1980.113299534213026No Hit
GGTATCAACGCAGAGTACATGGGGT1910.10929399512468671No Hit
GTACATGGGCAGTGGTATCAACGCA1880.10757733551539843No Hit
ATACAGGGTGACAGCCCCGTACACA1860.10643289577587291No Hit
GGATACCACGTGTCCCGCCCTACTC1800.10299957655729637No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTA400.005103733414.3125351
GGATAGT604.0768879E-412.66037212
TGGATAG604.0768879E-412.66037211
TGTCTTA550.003061125512.08490118
GTGGATA657.9943496E-411.68649810
CAAGCAG600.005862021411.0778266
TAGTGTC700.001486111710.851747515
AGTGTCT750.002638377510.12829816
GGTATCA19750.09.8074081
TACATAA5650.09.7585342
CATAAGC5600.09.6759124
GTACATA5650.09.626131
ACATAAG5700.09.5061593
TGGGGCA1054.4630945E-49.0431236
TGGGGTG1901.0291842E-88.9955276
AGCAGTG6550.08.9878988
TGGTTCA850.0073406918.9469735
GGGTGGA850.0074100488.9367338
ATAAGCA6100.08.8828045
CGCAGAA4100.08.8005029
Produced by FastQC (version 0.11.5)