##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512108_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 174758 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.781726730678997 31.0 31.0 34.0 28.0 34.0 2 30.86848098513373 31.0 31.0 34.0 27.0 34.0 3 30.841283374723904 31.0 31.0 34.0 27.0 34.0 4 34.56749333363852 37.0 35.0 37.0 32.0 37.0 5 34.20040856498701 37.0 35.0 37.0 30.0 37.0 6 34.23139999313336 37.0 35.0 37.0 30.0 37.0 7 34.052346673685896 37.0 35.0 37.0 30.0 37.0 8 33.986049279575184 37.0 35.0 37.0 30.0 37.0 9 35.35315693702148 39.0 35.0 39.0 30.0 39.0 10 35.2431247782648 39.0 35.0 39.0 29.0 39.0 11 35.19787363096396 39.0 35.0 39.0 29.0 39.0 12 35.02928621293446 39.0 35.0 39.0 28.0 39.0 13 34.85734558646814 39.0 35.0 39.0 27.0 39.0 14 35.72475652044542 39.0 36.0 41.0 27.0 41.0 15 35.561656690966934 39.0 35.0 41.0 27.0 41.0 16 35.4339143272411 39.0 35.0 41.0 27.0 41.0 17 35.63907231714714 39.0 36.0 41.0 27.0 41.0 18 35.59105734787535 39.0 35.0 41.0 27.0 41.0 19 35.65270831664359 39.0 35.0 41.0 27.0 41.0 20 35.63531855480149 39.0 35.0 41.0 27.0 41.0 21 35.568036942514794 39.0 35.0 41.0 27.0 41.0 22 35.424707309536615 39.0 35.0 41.0 27.0 41.0 23 35.294498678172104 39.0 35.0 41.0 27.0 41.0 24 35.15151809931448 39.0 35.0 41.0 27.0 41.0 25 34.82541571773538 38.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17477854032921059 1101 2 0.06711350224783885 1101 3 -0.11728318452460584 1101 4 -0.2638178256527439 1101 5 -0.1578429528033496 1101 6 -0.040392794346161054 1101 7 0.1421007165389767 1101 8 -0.2727089255789821 1101 9 -0.061226660821731116 1101 10 0.18124342314605002 1101 11 -0.38585613265034624 1101 12 -0.6796459171283047 1101 13 -0.2168405726637701 1101 14 -0.28108508150943834 1101 15 -0.4210601353944341 1101 16 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0.23792875687218284 1103 3 0.02284013130092788 1103 4 -0.08683677975185589 1103 5 -0.631878120649894 1103 6 -0.005270620333831744 1103 7 0.1372137560457105 1103 8 0.11797387871423837 1103 9 -0.127634516496542 1103 10 -0.017021020790920716 1103 11 -0.018055264872316457 1103 12 0.011699138820546295 1103 13 -0.10654141760554126 1103 14 0.2916484192898352 1103 15 0.20483618796706082 1103 16 -0.3310759228510207 1103 17 0.017659052810572007 1103 18 0.25432538758509793 1103 19 0.4688105190914271 1103 20 0.7363373142567156 1103 21 0.6183086947234102 1103 22 0.635972254537478 1103 23 0.03977349830014987 1103 24 0.5146859029605508 1103 25 -0.3439922101055757 1104 1 0.4281814441404599 1104 2 0.35499916467977854 1104 3 0.2797676321723088 1104 4 0.20555604004598393 1104 5 0.6732369020060887 1104 6 0.47290121654312856 1104 7 0.5498139651778118 1104 8 0.057282000010857814 1104 9 1.166631778379724 1104 10 0.7968514086269991 1104 11 0.6248516713423911 1104 12 0.6161344821457462 1104 13 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-0.23921670734112865 2315 1 -0.6183249079466542 2315 2 -0.5320244287866451 2315 3 -0.5060762879728813 2315 4 -0.7767488601355055 2315 5 -0.7414636424585197 2315 6 -0.6722893460703006 2315 7 -0.5354854903575799 2315 8 -0.7063296152341536 2315 9 -1.1232956263389724 2315 10 -0.6963427837505023 2315 11 -1.0160285464434509 2315 12 -1.0106803998869225 2315 13 -0.8720129864568733 2315 14 -1.5267747366818512 2315 15 -1.3460601353944313 2315 16 -1.0499329678384584 2315 17 -0.714699933259304 2315 18 -0.8441845461897373 2315 19 -0.6767706638270496 2315 20 -0.8688522267343828 2315 21 -0.6366011020338362 2315 22 -0.6539626621101604 2315 23 -0.9119278033669005 2315 24 -0.46010857066356436 2315 25 -0.7626665649811173 2316 1 -1.6562837511057182 2316 2 -1.3141434499212394 2316 3 -1.2427281233455183 2316 4 -1.5745797833835553 2316 5 -1.4197172575864414 2316 6 -1.2989578666487205 2316 7 -1.9217480042619144 2316 8 -1.5668691035545095 2316 9 -2.003996404981905 2316 10 -2.255992394429029 2316 11 -2.19539450862365 2316 12 -1.6826770628457695 2316 13 -1.7071075359563181 2316 14 -2.181808107093417 2316 15 -2.245948900689207 2316 16 -2.3088317442567003 2316 17 -2.2675364182426208 2316 18 -1.880474868770385 2316 19 -2.2186227216690924 2316 20 -2.442684262329486 2316 21 -2.5103497116000213 2316 22 -2.414390915725292 2316 23 -2.2080902060365304 2316 24 -2.1394745328437637 2316 25 -2.308689924269217 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3045.0 3 0.0 4 47.0 5 477.0 6 586.0 7 476.0 8 366.0 9 336.0 10 299.0 11 287.0 12 282.0 13 283.0 14 254.0 15 248.0 16 252.0 17 283.0 18 306.0 19 323.0 20 444.0 21 561.0 22 692.0 23 953.0 24 1193.0 25 1360.0 26 1575.0 27 1758.0 28 2080.0 29 2600.0 30 3253.0 31 4374.0 32 5651.0 33 7142.0 34 9269.0 35 12518.0 36 21671.0 37 32383.0 38 57131.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.55122456019317 18.73922042083477 16.24813153961136 16.461423479360697 2 16.33916616120578 23.72595245997322 39.266871902859954 20.668009475961043 3 18.690989825930714 30.88098971148674 32.64113803087698 17.786882431705557 4 11.335103400130468 20.76929239290905 34.859062246077436 33.03654196088305 5 10.079419157939338 40.89886225041943 33.05486048682169 15.966858104819545 6 25.288112704425547 37.258952379862436 19.935568042664713 17.517366873047298 7 21.758088327859095 39.571865093443506 21.2602570411655 17.4097895375319 8 26.522390963503817 32.24344522139187 21.097746598152874 20.13641721695144 9 28.149784273109102 15.825312718158827 20.59133201341283 35.43357099531924 10 19.41885350026894 29.373190354661876 27.408187321896566 23.799768823172617 11 28.98064752400462 27.23537692122821 21.66882202817611 22.115153526591058 12 26.00052644228018 30.88098971148674 26.806212018906145 16.31227182732693 13 30.291031025761338 24.47269939001362 28.293411460419556 16.94285812380549 14 24.737064969844013 26.657434852767825 29.370901475182826 19.234598702205336 15 25.38767896176427 28.887947905103058 29.134574668970803 16.58979846416187 16 18.111903317730803 31.414870849975397 30.24525343618032 20.227972396113483 17 17.18948488767324 31.815424758809325 29.814371874248963 21.180718479268474 18 16.252760960871605 26.647134895112096 37.98452717472161 19.115576969294683 19 18.26468602295746 28.901681181977363 36.60147174950503 16.232161045560144 20 19.07609379828105 26.838828551482624 37.812861213792786 16.27221643644354 21 20.177044827704595 27.677702880554822 36.31536181462366 15.829890477116928 22 19.201409949759096 27.03338330720196 35.93426338136165 17.83094336167729 23 19.902951510088236 26.406802549811744 36.425228029618104 17.265017910481923 24 17.99329342962107 27.559884180047376 35.89216841960699 18.554653970724562 25 18.18202626534291 29.095590970215447 36.42528110783669 16.297101656604962 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9129.0 1 9129.0 2 5327.5 3 1526.0 4 1526.0 5 1526.0 6 2216.0 7 2906.0 8 2906.0 9 2906.0 10 2427.0 11 1948.0 12 1948.0 13 1948.0 14 1438.0 15 928.0 16 928.0 17 928.0 18 2028.0 19 3128.0 20 3128.0 21 3128.0 22 4982.0 23 6836.0 24 6836.0 25 6836.0 26 10088.5 27 13341.0 28 13341.0 29 13341.0 30 13607.5 31 13874.0 32 13874.0 33 13874.0 34 12973.5 35 12073.0 36 12073.0 37 12073.0 38 11400.5 39 10728.0 40 10728.0 41 10728.0 42 15237.5 43 19747.0 44 19747.0 45 19747.0 46 19292.0 47 18837.0 48 18837.0 49 18837.0 50 19102.5 51 19368.0 52 19368.0 53 19368.0 54 17292.0 55 15216.0 56 15216.0 57 15216.0 58 12883.0 59 10550.0 60 10550.0 61 10550.0 62 8928.0 63 7306.0 64 7306.0 65 7306.0 66 5631.0 67 3956.0 68 3956.0 69 3956.0 70 3027.5 71 2099.0 72 2099.0 73 2099.0 74 1484.0 75 869.0 76 869.0 77 869.0 78 582.0 79 295.0 80 295.0 81 295.0 82 185.5 83 76.0 84 76.0 85 76.0 86 48.5 87 21.0 88 21.0 89 21.0 90 11.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4680758534659358 2 0.0 3 0.0 4 0.0 5 0.06580528502271714 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.002288879479051031 25 0.0017166596092882728 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 174758.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.14775861477014 #Duplication Level Percentage of deduplicated Percentage of total 1 75.6011069546232 31.10816099978256 2 13.966262915629477 11.493608304054751 3 4.938185762561015 6.095858272582657 4 2.0553755440904475 3.382963870037423 5 1.0735791069268104 2.2087686972842446 6 0.529836320905589 1.3080946222776642 7 0.35461486044862256 1.0214124675265224 8 0.2058156837113574 0.677508325799105 9 0.16965887441071353 0.6282974169995079 >10 0.8816698883310851 6.699550235182366 >50 0.10568913487880516 3.1689536387461517 >100 0.09039202325160968 7.522402407901212 >500 0.01251581860406903 3.721145813067213 >1k 0.01251581860406903 14.209363805948797 >5k 0.0027812930231264515 6.7539111228098285 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6774 3.87621739777292 No Hit TATCAACGCAGAGTACTTTTTTTTT 5029 2.877693725036908 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 4705 2.6922944872337746 No Hit GGTATCAACGCAGAGTACTTTTTTT 4552 2.604744847160073 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 4305 2.4634065393286715 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2441 1.3967887020908913 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2386 1.3653166092539397 No Hit GTACATAAGCAGTGGTATCAACGCA 2182 1.2485837558223372 No Hit GGTATCAACGCAGAAAAAAAAAAAA 1622 0.9281406287551929 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1609 0.9207017704482771 No Hit GCTTATGTACTCTGCGTTGATACCA 1030 0.5893864658556404 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 975 0.5579143730186886 No Hit GGTATCAACGCAGAGAAAAAAAAAA 954 0.5458977557536708 No Hit GTATCAACGCAGAGAAAAAAAAAAA 815 0.4663591938566474 No Hit ATCAACGCAGAGTACTTTTTTTTTT 811 0.46407031437759644 No Hit GTATCAACGCAGAAAAAAAAAAAAA 699 0.39998168896416764 No Hit GTGGTATCAACGCAGAGTACTTTTT 602 0.3444763615971801 No Hit CTTATGTACTCTGCGTTGATACCAC 581 0.3324597443321622 No Hit GAGTACATAAGCAGTGGTATCAACG 542 0.3101431694114146 No Hit TATCAACGCAGAGAAAAAAAAAAAA 524 0.299843211755685 No Hit GTGGTATCAACGCAGAGTACATGGG 491 0.280959956053514 No Hit GGTATCAACGCAGAGTACATGGGGG 458 0.262076700351343 No Hit TATCAACGCAGAAAAAAAAAAAAAA 425 0.243193444649172 No Hit TATGTACTCTGCGTTGATACCACTG 418 0.2391879055608327 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 415 0.2374712459515444 No Hit CATAAGCAGTGGTATCAACGCAGAG 380 0.21744355050984787 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 329 0.18826033715194726 No Hit CAACGCAGAGTACTTTTTTTTTTTT 319 0.18253813845431968 No Hit ACGCAGAGTACATAAGCAGTGGTAT 315 0.18024925897526864 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 314 0.1796770391055059 No Hit GTATCAACGCAGAGTACATAAGCAG 314 0.1796770391055059 No Hit GTACTGGTTCACTATCGGTCAGTCA 312 0.1785325993659804 No Hit GCGTTGATACCACTGCTTATGTACT 310 0.17738815962645488 No Hit TATCAACGCAGAGTACATAAGCAGT 301 0.17223818079859005 No Hit GGTATCAACGCAGAGTACATGGGGA 297 0.169949301319539 No Hit TCAACGCAGAGTACTTTTTTTTTTT 278 0.15907712379404662 No Hit AACGCAGAGTACTTTTTTTTTTTTT 268 0.15335492509641904 No Hit GGTATCAACGCAGAGTACATAAGCA 253 0.14477162704997767 No Hit CTGCTTATGTACTCTGCGTTGATAC 247 0.14133830783140114 No Hit GTGGTATCAACGCAGAAAAAAAAAA 216 0.12359949186875566 No Hit ACTCTGCGTTGATACCACTGCTTAT 207 0.11844951304089082 No Hit GTATCAACGCAGAGTACATGGGGGG 206 0.11787729317112808 No Hit AAGCAGTGGTATCAACGCAGAGTAC 201 0.11501619382231429 No Hit GTACATGGGGTGGTATCAACGCAGA 198 0.113299534213026 No Hit CCCATGTACTCTGCGTTGATACCAC 198 0.113299534213026 No Hit GGTATCAACGCAGAGTACATGGGGT 191 0.10929399512468671 No Hit GTACATGGGCAGTGGTATCAACGCA 188 0.10757733551539843 No Hit ATACAGGGTGACAGCCCCGTACACA 186 0.10643289577587291 No Hit GGATACCACGTGTCCCGCCCTACTC 180 0.10299957655729637 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.722198697627576E-4 0.0 0.0 0.0 0.0 7 5.722198697627576E-4 0.0 0.0 0.0 0.0 8 5.722198697627576E-4 0.0 0.0 0.0 0.0 9 5.722198697627576E-4 0.0 0.0 0.0 0.0 10 0.0011444397395255152 0.0 0.0 0.0 0.0 11 0.0011444397395255152 0.0 0.0 0.0 0.0 12 0.0011444397395255152 0.0 0.0 0.0 0.0 13 0.0011444397395255152 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCATTA 40 0.0051037334 14.312535 1 GGATAGT 60 4.0768879E-4 12.660372 12 TGGATAG 60 4.0768879E-4 12.660372 11 TGTCTTA 55 0.0030611255 12.084901 18 GTGGATA 65 7.9943496E-4 11.686498 10 CAAGCAG 60 0.0058620214 11.077826 6 TAGTGTC 70 0.0014861117 10.8517475 15 AGTGTCT 75 0.0026383775 10.128298 16 GGTATCA 1975 0.0 9.807408 1 TACATAA 565 0.0 9.758534 2 CATAAGC 560 0.0 9.675912 4 GTACATA 565 0.0 9.62613 1 ACATAAG 570 0.0 9.506159 3 TGGGGCA 105 4.4630945E-4 9.043123 6 TGGGGTG 190 1.0291842E-8 8.995527 6 AGCAGTG 655 0.0 8.987898 8 TGGTTCA 85 0.007340691 8.946973 5 GGGTGGA 85 0.007410048 8.936733 8 ATAAGCA 610 0.0 8.882804 5 CGCAGAA 410 0.0 8.800502 9 >>END_MODULE