FastQCFastQC Report
Thu 26 May 2016
SRR1512107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281310
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32710.25528560613746865No Hit
GGTATCAACGCAGAGTACTTTTTTT22760.17763070607425213No Hit
TATCAACGCAGAGTACTTTTTTTTT21560.16826529099125115No Hit
GTCCTAAAGTGTGTATTTCTCATTT14720.11488242501814548No Hit
CTTTAGGACGTGAAATATGGCGAGG14690.11464828964107046No Hit
GTCCTACAGTGGACATTTCTAAATT13840.10801445395727809No Hit
CTGTAGGACGTGGAATATGGCAAGA13530.10559505506083618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2500.012.4808144
GGTATCA8100.011.6099471
GCATAAG1052.1140131E-611.3131521
GACCGTG700.001551102110.80597
CTAATAG1002.5259253E-510.4006793
GTATTGC1156.283899E-610.3293991
TAAGACT1106.392129E-610.3147234
GGCGAGG4450.09.98635219
AGGACCT5400.09.980455
GTCAACG907.6354475E-49.8990082
ACTAATA1201.0429818E-59.8990072
GCTTTAA1201.0429818E-59.8990071
GGACGTG8800.09.7774976
AGGACGT9200.09.6607095
GGACCTG5950.09.5346186
TTAGGAC6250.09.5308053
CTAGTAC800.0046941489.4551633
CTTAGAC1107.1949544E-59.4551623
GTTCTAG1905.2114046E-99.3780071
GTCTTAT950.0012527399.3780071
Produced by FastQC (version 0.11.5)