##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512107_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1281310 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.355120930922258 31.0 28.0 33.0 16.0 34.0 2 25.856234634865878 31.0 25.0 33.0 10.0 34.0 3 27.504799775230037 31.0 26.0 34.0 16.0 34.0 4 31.730070786929 35.0 32.0 37.0 19.0 37.0 5 32.11358297367538 35.0 33.0 37.0 19.0 37.0 6 32.356219025840744 35.0 33.0 37.0 22.0 37.0 7 32.379761338005636 35.0 33.0 37.0 22.0 37.0 8 32.41321382023086 36.0 33.0 37.0 23.0 37.0 9 33.89256151907033 38.0 34.0 39.0 23.0 39.0 10 33.73248706402042 38.0 34.0 39.0 23.0 39.0 11 33.74294823266813 38.0 34.0 39.0 23.0 39.0 12 33.631476379642706 38.0 34.0 39.0 22.0 39.0 13 33.61627865231677 38.0 34.0 39.0 22.0 39.0 14 34.61543732586181 39.0 34.0 41.0 19.0 41.0 15 34.543225292864335 39.0 34.0 41.0 19.0 41.0 16 34.495435140598296 39.0 34.0 41.0 19.0 41.0 17 34.431090056270534 39.0 34.0 41.0 18.0 41.0 18 34.367765021735565 39.0 34.0 41.0 18.0 41.0 19 34.38028267944526 39.0 34.0 41.0 18.0 41.0 20 34.293398943269 39.0 34.0 41.0 18.0 41.0 21 34.218427234627065 39.0 34.0 41.0 18.0 41.0 22 34.09278784993483 39.0 34.0 41.0 15.0 41.0 23 33.962814619412946 39.0 34.0 41.0 10.0 41.0 24 33.835000897518945 39.0 34.0 41.0 2.0 41.0 25 33.69210963779257 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0544405511009174 1101 2 3.315300179087952 1101 3 1.7035949634929786 1101 4 1.1573018371052548 1101 5 0.9467188514318536 1101 6 0.6111890079967708 1101 7 0.5035552405348369 1101 8 0.3833810585819819 1101 9 0.3013377051016448 1101 10 0.16635467594900177 1101 11 0.4775996150077404 1101 12 0.2431550125237436 1101 13 0.35075950745584805 1101 14 0.4481438657489605 1101 15 0.37928489726950687 1101 16 0.4769580528068431 1101 17 0.4292290578664506 1101 18 0.21808589251220667 1101 19 0.22380328237375835 1101 20 0.20537756050918432 1101 21 0.20384296916084566 1101 22 0.35039037596341416 1101 23 0.14549425087209045 1101 24 -0.07244841884234177 1101 25 -0.23578539207439064 1102 1 1.343842909604298 1102 2 3.8217473437212703 1102 3 2.398663979605793 1102 4 1.4984425049855936 1102 5 1.1927524628222272 1102 6 1.0553144868979487 1102 7 0.670322895345592 1102 8 0.7326888595764629 1102 9 0.8974120740389608 1102 10 0.8758579773103037 1102 11 0.8317276752202218 1102 12 0.7500620581450264 1102 13 0.8040466036164844 1102 14 0.7916274132858447 1102 15 0.7565625648509524 1102 16 0.9934614628549383 1102 17 0.5875279737157086 1102 18 0.5785320517927062 1102 19 0.7608179279189571 1102 20 0.9583936189826758 1102 21 1.0073487974900601 1102 22 0.933908023981239 1102 23 0.9071468335419794 1102 24 1.0131546569938692 1102 25 0.9039994080777731 1103 1 0.8924080489346444 1103 2 2.766306755946239 1103 3 1.7596469378959725 1103 4 1.0923082686483419 1103 5 0.8414211705547956 1103 6 0.8239829348978418 1103 7 0.7134859198026646 1103 8 0.6644226725494633 1103 9 0.899095141741526 1103 10 0.7043344179337865 1103 11 0.7134428199075558 1103 12 0.7707218440603683 1103 13 0.7312545748121337 1103 14 0.7720117527045005 1103 15 0.7908323426821227 1103 16 0.6369978530253562 1103 17 0.915576829430961 1103 18 0.9489433060092978 1103 19 0.9745486955263942 1103 20 0.9201090234026807 1103 21 1.0575408373330504 1103 22 0.9764405473815287 1103 23 1.1900710338088558 1103 24 0.8857505876322591 1103 25 1.1967668272116256 1104 1 1.499941148325295 1104 2 3.964443577214329 1104 3 2.5233396567660336 1104 4 1.887768850501292 1104 5 1.4240651665842137 1104 6 1.1509204980582055 1104 7 0.9514373704030206 1104 8 0.8889602858861139 1104 9 0.9765407078279864 1104 10 1.0068756805714614 1104 11 0.9472489666309585 1104 12 1.068612839912511 1104 13 0.9216383914229169 1104 14 1.1402378736869565 1104 15 0.8344569139596487 1104 16 0.971302498620112 1104 17 1.0014269239275038 1104 18 0.9790541994480435 1104 19 0.9980752310392376 1104 20 0.7296535263790176 1104 21 0.788581208125585 1104 22 0.7084946840133597 1104 23 1.18664381406807 1104 24 1.166140042675515 1104 25 1.2105608266650663 1105 1 1.3575775011094535 1105 2 3.7644141643818436 1105 3 2.2643178241988906 1105 4 1.7121496273077348 1105 5 1.4092246081342097 1105 6 0.9649027925175773 1105 7 0.7635576725798785 1105 8 0.798229081639839 1105 9 0.7993733112759358 1105 10 0.7095014835017892 1105 11 0.6638354043615919 1105 12 0.5487828682362021 1105 13 0.6276239028339248 1105 14 0.6950863452964953 1105 15 0.8027931998573052 1105 16 0.7238888895855737 1105 17 0.9362024295605522 1105 18 0.7377440608200772 1105 19 1.0223432791655256 1105 20 0.9157352263668983 1105 21 0.905867646656155 1105 22 1.1498139295434342 1105 23 1.0098197723387727 1105 24 1.0423169405569936 1105 25 0.9588066711794809 1106 1 0.6214036383492392 1106 2 2.9255462640991396 1106 3 1.7849763952603155 1106 4 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1.0276709181170247 1107 17 0.5438626443360874 1107 18 0.34821266357857894 1107 19 0.7916795753219574 1107 20 0.8892831137819499 1107 21 0.9060558826633098 1107 22 1.1749856876152975 1107 23 1.2290774231417458 1107 24 1.2514506411545128 1107 25 1.2115784900929398 1108 1 0.6054922181442599 1108 2 1.8666712030937695 1108 3 1.0616149994574826 1108 4 0.5697046788909041 1108 5 0.4798177440001723 1108 6 0.4742610254483992 1108 7 0.40909682081135657 1108 8 0.3276888892146985 1108 9 0.4837310644420967 1108 10 0.20226268149107085 1108 11 0.1546498867068209 1108 12 0.4285589253835127 1108 13 0.32161081485411813 1108 14 0.1828331559903944 1108 15 0.14414236580056183 1108 16 0.4963393355409309 1108 17 0.13212759078970038 1108 18 0.3610644612034335 1108 19 0.3661996387564699 1108 20 0.2161988483529882 1108 21 0.455202692843379 1108 22 0.35740640276974744 1108 23 0.5841629745251424 1108 24 0.6707713871851553 1108 25 0.503231185008552 1109 1 0.5099021911712356 1109 2 2.866503813544387 1109 3 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-1.7729036264552107 2316 4 -2.199430340495688 2316 5 -2.374513358960993 2316 6 -2.3060934177295707 2316 7 -2.3755355493127865 2316 8 -2.63095829388725 2316 9 -2.997395980926818 2316 10 -3.1073648002550094 2316 11 -3.306728588214611 2316 12 -3.2394696513592542 2316 13 -3.3746449260093847 2316 14 -3.6979610027555125 2316 15 -3.8848965075356006 2316 16 -3.730807616257273 2316 17 -3.612522139037992 2316 18 -3.6074152770656482 2316 19 -3.863900847219842 2316 20 -3.7709368482528127 2316 21 -4.058620686480452 2316 22 -3.971661085462376 2316 23 -3.907731379278971 2316 24 -3.797804210985401 2316 25 -4.248427763584786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67087.0 3 3.0 4 533.0 5 2660.0 6 3247.0 7 3034.0 8 2678.0 9 2615.0 10 2532.0 11 2424.0 12 2421.0 13 2347.0 14 2441.0 15 2596.0 16 2620.0 17 2868.0 18 3233.0 19 4156.0 20 5417.0 21 6854.0 22 8527.0 23 10132.0 24 11639.0 25 13270.0 26 14733.0 27 16508.0 28 19070.0 29 22721.0 30 27817.0 31 35085.0 32 43925.0 33 56549.0 34 73237.0 35 97795.0 36 137183.0 37 189204.0 38 382149.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.603429302822896 19.402330427453155 12.956895677080487 25.037344592643468 2 16.19401143589249 22.139188440564574 38.7995908048757 22.86720931866724 3 18.14697458070256 25.16112416199046 30.426672702156388 26.26522855515059 4 12.537481171613427 17.520662447026872 38.48826591535225 31.453590466007448 5 13.24675527389937 37.768065495469486 35.88530488328352 13.099874347347637 6 30.90212360787007 37.41225776744114 18.655048348955365 13.03057027573343 7 27.238295182274392 32.141870429482324 22.97617282312633 17.64366156511695 8 24.80352016932776 36.38018203358771 21.32393456031443 17.492363236770096 9 26.149253498372758 15.689021392168954 20.880036837299325 37.281688272158966 10 15.254075906689248 28.857107179371113 34.60544286706574 21.283374046873902 11 33.08824562361958 23.032209223372956 25.199444318705076 18.680100834302394 12 22.8377207701493 25.156675589826037 31.88861399661284 20.11698964341182 13 28.419586204743585 22.328320234759737 26.141605076054976 23.110488484441703 14 21.083159617484334 22.4299094596377 28.050030164511707 28.436900758366264 15 22.927316574443342 30.586821300075705 23.886178988691263 22.599683136789693 16 22.023085748179597 28.426298085553064 27.68003059368927 21.870585572578065 17 21.627709141425573 28.650990002419398 27.945149885663888 21.776150970491138 18 21.371565038905498 27.728964887497952 29.698355589201675 21.20111448439488 19 23.09458530299873 27.273670909983462 28.099154848674285 21.532588938343522 20 23.166134659059868 27.520740492152562 28.38672920682739 20.926395641960184 21 22.764826622753276 27.10499410759301 27.75308083133668 22.377098438317038 22 22.90957592520762 27.908690110957014 27.847736725078377 21.33399723875699 23 22.164312422588566 27.673235760784138 28.12191963961686 22.040532177010434 24 22.60424503710271 27.097622117398785 28.574004064596494 21.724128780902017 25 22.48495680202293 27.419515964130458 28.280119565132562 21.81540766871405 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1325.0 1 1325.0 2 1021.0 3 717.0 4 717.0 5 717.0 6 1376.5 7 2036.0 8 2036.0 9 2036.0 10 2744.0 11 3452.0 12 3452.0 13 3452.0 14 5375.5 15 7299.0 16 7299.0 17 7299.0 18 12458.0 19 17617.0 20 17617.0 21 17617.0 22 26828.0 23 36039.0 24 36039.0 25 36039.0 26 50169.5 27 64300.0 28 64300.0 29 64300.0 30 81178.5 31 98057.0 32 98057.0 33 98057.0 34 110849.0 35 123641.0 36 123641.0 37 123641.0 38 133718.0 39 143795.0 40 143795.0 41 143795.0 42 151418.5 43 159042.0 44 159042.0 45 159042.0 46 160740.5 47 162439.0 48 162439.0 49 162439.0 50 156947.5 51 151456.0 52 151456.0 53 151456.0 54 136345.5 55 121235.0 56 121235.0 57 121235.0 58 103614.0 59 85993.0 60 85993.0 61 85993.0 62 69864.0 63 53735.0 64 53735.0 65 53735.0 66 41046.5 67 28358.0 68 28358.0 69 28358.0 70 20809.5 71 13261.0 72 13261.0 73 13261.0 74 9078.0 75 4895.0 76 4895.0 77 4895.0 78 3354.0 79 1813.0 80 1813.0 81 1813.0 82 1193.5 83 574.0 84 574.0 85 574.0 86 370.5 87 167.0 88 167.0 89 167.0 90 107.0 91 47.0 92 47.0 93 47.0 94 30.5 95 14.0 96 14.0 97 14.0 98 8.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.483224200232574 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 6.243610055333994E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.341353770750248E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 7.804512569167493E-5 20 0.0 21 0.0 22 2.341353770750248E-4 23 5.463158798417245E-4 24 1.5609025138334985E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1281310.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.16344055973153 #Duplication Level Percentage of deduplicated Percentage of total 1 86.76055156560535 68.68263766793321 2 9.777958804652167 15.481137212551706 3 2.0411245063556493 4.847473156016903 4 0.628997601435691 1.9917445693387208 5 0.27497174788224044 1.088385480954061 6 0.137336499975808 0.6523217911509867 7 0.08597982150462954 0.4764520942312648 8 0.061917839754360056 0.3921303381585011 9 0.03831305950198097 0.27296942476918745 >10 0.16949431397942089 2.3116338275237185 >50 0.01264395895051294 0.7004370932901133 >100 0.008826089603576111 1.4230682033790887 >500 9.916793676530058E-4 0.46733878077131624 >1k 8.925114308877053E-4 1.212270359931239 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3271 0.25528560613746865 No Hit GGTATCAACGCAGAGTACTTTTTTT 2276 0.17763070607425213 No Hit TATCAACGCAGAGTACTTTTTTTTT 2156 0.16826529099125115 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1472 0.11488242501814548 No Hit CTTTAGGACGTGAAATATGGCGAGG 1469 0.11464828964107046 No Hit GTCCTACAGTGGACATTTCTAAATT 1384 0.10801445395727809 No Hit CTGTAGGACGTGGAATATGGCAAGA 1353 0.10559505506083618 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 7.804512569167493E-5 13 0.0 0.0 0.0 0.0 7.804512569167493E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 250 0.0 12.480814 4 GGTATCA 810 0.0 11.609947 1 GCATAAG 105 2.1140131E-6 11.313152 1 GACCGTG 70 0.0015511021 10.8059 7 CTAATAG 100 2.5259253E-5 10.400679 3 GTATTGC 115 6.283899E-6 10.329399 1 TAAGACT 110 6.392129E-6 10.314723 4 GGCGAGG 445 0.0 9.986352 19 AGGACCT 540 0.0 9.98045 5 GTCAACG 90 7.6354475E-4 9.899008 2 ACTAATA 120 1.0429818E-5 9.899007 2 GCTTTAA 120 1.0429818E-5 9.899007 1 GGACGTG 880 0.0 9.777497 6 AGGACGT 920 0.0 9.660709 5 GGACCTG 595 0.0 9.534618 6 TTAGGAC 625 0.0 9.530805 3 CTAGTAC 80 0.004694148 9.455163 3 CTTAGAC 110 7.1949544E-5 9.455162 3 GTTCTAG 190 5.2114046E-9 9.378007 1 GTCTTAT 95 0.001252739 9.378007 1 >>END_MODULE