FastQCFastQC Report
Thu 26 May 2016
SRR1512107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281310
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39950.3117902771382413No Hit
GGTATCAACGCAGAGTACTTTTTTT28860.22523823274617386No Hit
TATCAACGCAGAGTACTTTTTTTTT26930.2101755234876806No Hit
GTCCTAAAGTGTGTATTTCTCATTT16270.12697941950035513No Hit
CTGTAGGACGTGGAATATGGCAAGA16140.12596483286636334No Hit
GTCCTACAGTGGACATTTCTAAATT15730.12276498271300466No Hit
CTTTAGGACGTGAAATATGGCGAGG15140.11816032029719582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2450.013.187444
TCGAGGA658.049846E-411.6874329
GGTATCA9950.011.509551
AAGGCGT1001.9373074E-611.3956926
AGGCGTG1104.989288E-711.223037
GGTATAG803.6144073E-410.7362521
GGCGTGC803.7828894E-410.6834618
CTGTACT2200.010.3665974
TAAGGGT1201.516868E-610.2946074
CCTGTAC1701.54796E-910.0616983
TAGAACA3000.09.8194714
CCAGGAC3550.09.6365563
TGGCGAG9600.09.59495618
GTTTAAG1201.6099584E-59.5433351
GTCTTAA1803.8598955E-99.5433351
CTTATAC1304.2192314E-69.5027143
AAAGGCG1304.2192314E-69.5027145
TCTATAC1304.2192314E-69.5027143
TATACTG1804.152753E-99.5027135
CGAAATC1002.7655475E-49.49603813
Produced by FastQC (version 0.11.5)