FastQCFastQC Report
Thu 26 May 2016
SRR1512106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3237520
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58840.18174405100200153No Hit
TATCAACGCAGAGTACTTTTTTTTT37540.11595295164199759No Hit
GGTATCAACGCAGAGTACTTTTTTT35420.1094047295460723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16550.012.9823421
GTCCTAT2750.011.5214891
TAGGACC6750.010.7856114
GTCCTAC14050.010.570761
GTATTAA3600.010.1763141
TATACTG5300.010.1684975
GTATTAG2350.010.1119441
TCCAACG2550.010.0110918
AATCCCG3900.09.93978619
GTATCAA32050.09.9167251
TAAACGT1054.3282795E-59.9051534
TGAGTTA8750.09.83311612
GGACGTG13200.09.7414316
TTAGGAC11400.09.5378563
GATATAC7850.09.4598211
AGGACGT13600.09.454925
ACTGTTC9900.09.4549198
TCCTACA16900.09.4325792
AATACTG4650.09.3532535
GTCAACG850.00529434259.3188512
Produced by FastQC (version 0.11.5)