##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512106_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3237520 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.591236193135487 31.0 28.0 33.0 16.0 34.0 2 25.14771368207764 31.0 22.0 33.0 2.0 34.0 3 26.75869647137315 31.0 25.0 33.0 15.0 34.0 4 30.87449745484198 35.0 32.0 37.0 17.0 37.0 5 31.22780060045961 35.0 32.0 37.0 17.0 37.0 6 31.447712446564037 35.0 32.0 37.0 17.0 37.0 7 31.457762423089278 35.0 32.0 37.0 16.0 37.0 8 31.48347500555981 35.0 32.0 37.0 15.0 37.0 9 32.89289765005313 38.0 33.0 39.0 16.0 39.0 10 32.71761471743804 37.0 32.0 39.0 11.0 39.0 11 32.72581204131558 37.0 33.0 39.0 11.0 39.0 12 32.589838209493685 37.0 32.0 39.0 10.0 39.0 13 32.566618584595616 37.0 32.0 39.0 10.0 39.0 14 33.492649002940524 38.0 33.0 41.0 10.0 41.0 15 33.40430452939287 38.0 33.0 41.0 10.0 41.0 16 33.35203149324174 38.0 33.0 41.0 8.0 41.0 17 33.26427450641231 38.0 32.0 41.0 2.0 41.0 18 33.19150893276335 38.0 32.0 41.0 2.0 41.0 19 33.19385023104105 39.0 32.0 41.0 2.0 41.0 20 33.10249233981566 39.0 32.0 41.0 2.0 41.0 21 33.014730719810224 39.0 32.0 40.0 2.0 41.0 22 32.871712298302405 38.0 32.0 40.0 2.0 41.0 23 32.7335105265759 38.0 32.0 40.0 2.0 41.0 24 32.58003039363464 38.0 32.0 40.0 2.0 41.0 25 32.42223183177247 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2605057410938478 1101 2 3.336370747309207 1101 3 1.971273259220581 1101 4 1.4463973033216604 1101 5 1.1251414477739772 1101 6 0.7666399286915748 1101 7 0.6384864385488278 1101 8 0.7951428062412162 1101 9 0.7891214010893606 1101 10 0.4195652522180424 1101 11 0.6773007960232249 1101 12 0.4412460112831056 1101 13 0.46840435785511403 1101 14 0.5007931874770293 1101 15 0.6359447379749525 1101 16 0.6213933513002132 1101 17 0.5035510178449343 1101 18 0.443908679917115 1101 19 0.4212895799087306 1101 20 0.4840230275265043 1101 21 0.4834146779465698 1101 22 0.4195080565253164 1101 23 0.43386920847243715 1101 24 0.3488253038490541 1101 25 0.27471860349938027 1102 1 1.604799195088198 1102 2 4.032008028444615 1102 3 2.641268682822247 1102 4 1.884693020031257 1102 5 1.6422492968003723 1102 6 1.5079131311060792 1102 7 1.359397131109727 1102 8 1.2695132623670062 1102 9 1.4168399626569084 1102 10 1.3569130312942903 1102 11 1.459944360979712 1102 12 1.3708150910201553 1102 13 1.430678624383951 1102 14 1.3649796261332625 1102 15 1.2858493017598178 1102 16 1.5812449320849922 1102 17 1.2310025121985362 1102 18 1.3622294993601045 1102 19 1.3108378741884934 1102 20 1.4403418729292596 1102 21 1.4036457207464963 1102 22 1.5220200216547468 1102 23 1.5638135443068322 1102 24 1.2493836158797578 1102 25 1.1352540956420825 1103 1 1.2400392317842233 1103 2 2.9821570391624377 1103 3 2.066479954543631 1103 4 1.3575222810360472 1103 5 1.228957853567465 1103 6 1.1749036043802548 1103 7 0.9892214585653569 1103 8 0.7680002098841996 1103 9 1.0032803783311692 1103 10 0.8729443908375387 1103 11 0.9217624481356452 1103 12 0.7714522502309364 1103 13 0.8538972558889455 1103 14 1.0227482477398837 1103 15 0.882271130708439 1103 16 0.9336576462943285 1103 17 0.9619829125868975 1103 18 1.0219558802044162 1103 19 1.130792037084106 1103 20 1.0914281633258582 1103 21 1.294690302135649 1103 22 1.2537378614952885 1103 23 1.3529430056251641 1103 24 1.1130998250602673 1103 25 1.239546575632339 1104 1 1.874250724628336 1104 2 4.2316833281454365 1104 3 2.804668428421042 1104 4 2.207092603127073 1104 5 1.8454093938131209 1104 6 1.5646202503155138 1104 7 1.36298442246024 1104 8 1.4954067612801722 1104 9 1.6479689100400776 1104 10 1.6800535056908998 1104 11 1.5838421297985974 1104 12 1.5393139659799786 1104 13 1.4959947334209005 1104 14 1.6087012804441017 1104 15 1.3688618318088572 1104 16 1.5092284026289065 1104 17 1.5736001451567816 1104 18 1.6474507453758562 1104 19 1.6286635334589832 1104 20 1.4783454602206092 1104 21 1.6548080754808083 1104 22 1.590580484732186 1104 23 1.679468286107948 1104 24 1.8608666159801146 1104 25 1.7496056043472024 1105 1 1.3917301489934566 1105 2 3.711829992802464 1105 3 2.2089861290033 1105 4 1.6752408423268434 1105 5 1.2456088944274768 1105 6 0.9648489199683468 1105 7 0.583059452251188 1105 8 0.6496705869410206 1105 9 0.606543314745366 1105 10 0.6433517337449786 1105 11 0.46738615821841023 1105 12 0.47751033409323895 1105 13 0.4133864555599587 1105 14 0.5540306003518722 1105 15 0.5069021114218941 1105 16 0.5176360121384036 1105 17 0.5696492525500645 1105 18 0.5742701153734018 1105 19 0.7549053760056808 1105 20 0.7767557476234401 1105 21 0.7096809877361423 1105 22 0.8098326354609995 1105 23 0.7751017590917115 1105 24 0.7705480539844416 1105 25 0.9669121119206636 1106 1 0.4950671460703937 1106 2 2.8389995301475253 1106 3 1.5442479625798313 1106 4 0.8583042364832068 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-1.6799570752405408 2316 4 -2.4045741790720108 2316 5 -2.2145821284977814 2316 6 -2.5238764657751247 2316 7 -2.493346236098443 2316 8 -2.582269905713172 2316 9 -2.8556093281144648 2316 10 -2.901990446027707 2316 11 -3.3502040783876303 2316 12 -3.330872057147225 2316 13 -3.421178722169632 2316 14 -3.602348877357244 2316 15 -3.883112776556164 2316 16 -3.5972820482741987 2316 17 -3.5331181844197523 2316 18 -3.4380532030441024 2316 19 -3.7685330923570177 2316 20 -3.7879052897441348 2316 21 -3.7673645076407816 2316 22 -3.7018918213506353 2316 23 -3.803814969898344 2316 24 -3.711066894715401 2316 25 -4.086774438344019 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 229304.0 3 5.0 4 1939.0 5 8956.0 6 10300.0 7 9352.0 8 8574.0 9 8280.0 10 7831.0 11 7504.0 12 7479.0 13 7464.0 14 7416.0 15 7807.0 16 7997.0 17 8590.0 18 9831.0 19 12272.0 20 15669.0 21 20288.0 22 24333.0 23 28893.0 24 33175.0 25 37286.0 26 41407.0 27 45826.0 28 52666.0 29 62200.0 30 75140.0 31 92999.0 32 115545.0 33 144923.0 34 187338.0 35 244593.0 36 336619.0 37 459341.0 38 858378.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52646470137636 18.572765573648965 12.733264968247301 25.16750475672737 2 16.4798525023653 21.60280763041492 38.41902590103749 23.498313966182284 3 18.602201685240555 24.67388618448689 29.786873903481677 26.937038226790875 4 12.568107687365638 16.832235785415996 38.027996738244084 32.57165978897428 5 13.611869579184066 37.5976982381576 35.3561676839062 13.434264498752132 6 31.868590773184412 36.95442808075317 18.140088709876697 13.036892436185724 7 28.057371074155522 31.735587733820946 22.22472756925054 17.982313622772985 8 25.65280026588302 36.04673215312737 20.618752536668566 17.681715044321045 9 26.677086164718673 15.275612196990288 19.816310015073267 38.23099162321777 10 15.142825372507351 28.844578566310012 34.17835256616175 21.834243495020882 11 34.61606414786627 22.502409251525858 24.09965034965035 18.781876250957524 12 23.121370678791173 24.609886579851246 31.44388297215152 20.824859769206057 13 28.915157280881665 21.60536460006425 25.826465936889964 23.653012182164126 14 21.680110159005306 21.301423134255977 27.40594445964821 29.612522247090507 15 23.511514986780004 29.483431762583706 23.568163285477773 23.436889965158517 16 23.037201314586472 27.85938001927401 26.52681682275322 22.576601843386296 17 22.456077491413183 27.75473201709951 27.229484296622108 22.559706194865207 18 22.168851466554646 27.52659443030468 28.410449974054213 21.89410412908646 19 23.731727492143666 26.88291077660205 27.143666405766893 22.241695325487395 20 23.720253428863362 27.044746651445124 27.466489740900123 21.768510178791395 21 23.760640095282866 26.48411530067169 26.878553262097693 22.87669134194775 22 23.536895752895752 26.90437065637066 27.190270270270272 22.36846332046332 23 23.382997137916824 26.74512633505709 27.00758177137806 22.864294755648025 24 23.354755132953947 26.61061951276165 27.425689850724876 22.608935503559525 25 23.37826484469594 26.684931676097754 27.277545775778993 22.659257703427315 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 395.0 1 395.0 2 635.5 3 876.0 4 876.0 5 876.0 6 2104.5 7 3333.0 8 3333.0 9 3333.0 10 4874.0 11 6415.0 12 6415.0 13 6415.0 14 10729.5 15 15044.0 16 15044.0 17 15044.0 18 26048.0 19 37052.0 20 37052.0 21 37052.0 22 56909.0 23 76766.0 24 76766.0 25 76766.0 26 107924.5 27 139083.0 28 139083.0 29 139083.0 30 178714.0 31 218345.0 32 218345.0 33 218345.0 34 250492.5 35 282640.0 36 282640.0 37 282640.0 38 312804.0 39 342968.0 40 342968.0 41 342968.0 42 368335.0 43 393702.0 44 393702.0 45 393702.0 46 405154.0 47 416606.0 48 416606.0 49 416606.0 50 411308.5 51 406011.0 52 406011.0 53 406011.0 54 371700.5 55 337390.0 56 337390.0 57 337390.0 58 291795.5 59 246201.0 60 246201.0 61 246201.0 62 202469.5 63 158738.0 64 158738.0 65 158738.0 66 124351.5 67 89965.0 68 89965.0 69 89965.0 70 65540.0 71 41115.0 72 41115.0 73 41115.0 74 28844.5 75 16574.0 76 16574.0 77 16574.0 78 11167.5 79 5761.0 80 5761.0 81 5761.0 82 3813.5 83 1866.0 84 1866.0 85 1866.0 86 1180.0 87 494.0 88 494.0 89 494.0 90 307.5 91 121.0 92 121.0 93 121.0 94 83.5 95 46.0 96 46.0 97 46.0 98 29.5 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.507925819763275 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.8532704045071536E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 5.559811213521461E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 1.8532704045071536E-4 20 1.235513603004769E-4 21 6.177568015023845E-5 22 6.177568015023845E-4 23 5.559811213521461E-4 24 2.471027206009538E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3237520.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.18665536704262 #Duplication Level Percentage of deduplicated Percentage of total 1 84.8367913552505 62.08921011362418 2 10.023147408665965 14.671212701822045 3 2.4869665699201366 5.460382957863033 4 0.9734658605228637 2.849788417826736 5 0.49401173961891615 1.8077533467381408 6 0.28889605240005106 1.2686001494340975 7 0.19504103527577651 0.9992080721811621 8 0.13535075017741185 0.7924694965523938 9 0.0979646529759906 0.6452734765955174 >10 0.4373989558478012 5.444743724472011 >50 0.018874029408460134 0.948207999335857 >100 0.010517975626214787 1.4568083637870515 >500 7.65542096973668E-4 0.4207973931192607 >1k 7.6554209650876E-4 0.9623962269212049 >5k 4.2530116472708886E-5 0.18314755972717 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5884 0.18174405100200153 No Hit TATCAACGCAGAGTACTTTTTTTTT 3754 0.11595295164199759 No Hit GGTATCAACGCAGAGTACTTTTTTT 3542 0.1094047295460723 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.088784007511923E-5 0.0 5 0.0 0.0 0.0 3.088784007511923E-5 0.0 6 0.0 0.0 0.0 6.177568015023845E-5 3.088784007511923E-5 7 0.0 0.0 0.0 9.266352022535768E-5 3.088784007511923E-5 8 0.0 0.0 0.0 9.266352022535768E-5 3.088784007511923E-5 9 0.0 0.0 0.0 1.235513603004769E-4 6.177568015023845E-5 10 0.0 0.0 0.0 1.235513603004769E-4 6.177568015023845E-5 11 0.0 0.0 0.0 1.235513603004769E-4 6.177568015023845E-5 12 0.0 0.0 0.0 1.235513603004769E-4 9.266352022535768E-5 13 0.0 0.0 0.0 1.235513603004769E-4 1.235513603004769E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1655 0.0 12.982342 1 GTCCTAT 275 0.0 11.521489 1 TAGGACC 675 0.0 10.785611 4 GTCCTAC 1405 0.0 10.57076 1 GTATTAA 360 0.0 10.176314 1 TATACTG 530 0.0 10.168497 5 GTATTAG 235 0.0 10.111944 1 TCCAACG 255 0.0 10.01109 18 AATCCCG 390 0.0 9.939786 19 GTATCAA 3205 0.0 9.916725 1 TAAACGT 105 4.3282795E-5 9.905153 4 TGAGTTA 875 0.0 9.833116 12 GGACGTG 1320 0.0 9.741431 6 TTAGGAC 1140 0.0 9.537856 3 GATATAC 785 0.0 9.459821 1 AGGACGT 1360 0.0 9.45492 5 ACTGTTC 990 0.0 9.454919 8 TCCTACA 1690 0.0 9.432579 2 AATACTG 465 0.0 9.353253 5 GTCAACG 85 0.0052943425 9.318851 2 >>END_MODULE