FastQCFastQC Report
Thu 26 May 2016
SRR1512106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3237520
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72030.22248511206108382No Hit
TATCAACGCAGAGTACTTTTTTTTT47670.14724233363809336No Hit
GGTATCAACGCAGAGTACTTTTTTT44910.13871728977736045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17350.012.5923871
TCCAACG3250.011.10458618
TAGGACC5750.010.906694
TGTAGGA16550.010.1623032
ATACCGT750.002656749410.1297186
GTATCAA35650.010.0088481
AGGACGT13750.09.8821215
TAGGACG14100.09.569434
GGACGTG14400.09.5625596
GATATAC7100.09.540521
TTAGGAC10000.09.502043
GTAGGAC14850.09.4700473
TGAGTTA7950.09.1976912
CTGTAGG15550.09.141721
GACGTGA8150.09.088787
GGCGAGG6600.09.06578619
GTATTAA4000.09.0634941
AGAACAG12500.08.9699265
GAGTATA2053.8398866E-98.8424331
GTCCTAC14750.08.7966821
Produced by FastQC (version 0.11.5)