FastQCFastQC Report
Thu 26 May 2016
SRR1512105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529029
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45080.17825022963358664No Hit
TATCAACGCAGAGTACTTTTTTTTT28950.11447081073408015No Hit
GGTATCAACGCAGAGTACTTTTTTT27430.10846059890970014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAA457.00957E-414.70853411
AGGACGT11600.011.7380775
TAGGACC5300.011.59658054
GGACGTG11150.011.3637696
TAGCCCG752.1668806E-411.3468085
TTAGTAC1251.9646905E-811.34680753
TAGGACG12750.011.1984844
GTAGGAC13750.010.7278913
TGTAGGA13800.010.6842522
GACGTGA6400.010.6376327
CCAACGA803.9397174E-410.63742319
TTAGGAC8300.010.5949123
GGTATCA13950.010.5691481
TGGCGAG12600.010.50609618
TCCAACG1356.09798E-810.50609618
ACGTGAA7500.010.3382028
CTGTAGG13400.010.26450351
GGCGAGG6600.010.17181119
CGTGAAA7550.010.1442979
GACGTGG5900.010.0967357
Produced by FastQC (version 0.11.5)