FastQCFastQC Report
Thu 26 May 2016
SRR1512105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529029
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56780.2245130443344066No Hit
TATCAACGCAGAGTACTTTTTTTTT37130.14681524015738848No Hit
GGTATCAACGCAGAGTACTTTTTTT36700.14511498286496516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG604.1058759E-412.6621739
GGTATCA17000.012.2300161
TTAACGT550.003064792812.0933134
GTATTAG1658.6947693E-1010.4041831
TGTACCG1201.5207625E-610.2937135
GTCCTAT1951.4551915E-1110.2707971
TAGGACC5050.010.1604364
GCCGGTT750.002656361310.12973811
GTCTTAT2450.010.1210781
GTCGTAG1053.9344628E-59.9913191
ATTATAC3000.09.8186193
GTCCTAC12500.09.6134661
GTCCTAA6350.09.6122651
ATATCGA900.00111744169.4968176
ACCGTCC900.00111744169.4968178
CGTGCGC1002.7662134E-49.49662910
GCGTGCG1002.7662134E-49.4966299
GTAGAAC4700.09.0975543
TAGACTG2701.8189894E-128.7980465
TAGGACT3350.08.7927934
Produced by FastQC (version 0.11.5)