FastQCFastQC Report
Thu 26 May 2016
SRR1512104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611304
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44170.27412580121442015No Hit
TATCAACGCAGAGTACTTTTTTTTT30470.1891014979172149No Hit
GGTATCAACGCAGAGTACTTTTTTT30000.18618460576030346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAT400.005441704814.1850213
CTAGGAC2200.011.6059273
TAGGACC2600.011.2752734
GGCGAGG4300.011.216063519
GGTATCA10100.011.0593261
GACGTGA4750.010.5516657
GTGCTAT1603.2105163E-910.502691
GGTATGC951.1003071E-410.4051421
GGACGTG7750.010.3718436
GTCCTAC5200.010.2650731
AGGACGT8050.09.9853145
TTAAGAC1804.274625E-109.9820533
GTCATAC1104.4226523E-59.8848851
TGGCGAG7650.09.76572218
GTATCAA19450.09.6561851
GTATTAA2154.9112714E-119.6550041
ACGTGAA5300.09.6351098
GTCCTAA5250.09.602461
CTAGGCC900.0011610819.4566813
TAGGACG8600.09.456684
Produced by FastQC (version 0.11.5)