##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512104_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1611304 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.191742216242247 31.0 28.0 33.0 16.0 34.0 2 25.737704989250943 31.0 24.0 33.0 7.0 34.0 3 27.357758684891245 31.0 26.0 34.0 16.0 34.0 4 31.556115419560804 35.0 32.0 37.0 19.0 37.0 5 31.92763438804844 35.0 32.0 37.0 19.0 37.0 6 32.15591657440185 35.0 33.0 37.0 19.0 37.0 7 32.17795090187823 35.0 33.0 37.0 19.0 37.0 8 32.211581427216714 35.0 33.0 37.0 21.0 37.0 9 33.67225365294197 38.0 34.0 39.0 22.0 39.0 10 33.513660985139985 38.0 33.0 39.0 19.0 39.0 11 33.52444231504421 38.0 33.0 39.0 20.0 39.0 12 33.4049862719884 38.0 33.0 39.0 19.0 39.0 13 33.38881800082418 38.0 33.0 39.0 18.0 39.0 14 34.355562947773976 39.0 34.0 41.0 18.0 41.0 15 34.276712526003784 39.0 33.0 41.0 17.0 41.0 16 34.23179114555664 39.0 33.0 41.0 17.0 41.0 17 34.16055195046993 39.0 33.0 41.0 17.0 41.0 18 34.10799886303268 39.0 33.0 41.0 17.0 41.0 19 34.12186837493111 39.0 34.0 41.0 18.0 41.0 20 34.029500330167366 39.0 34.0 41.0 16.0 41.0 21 33.94838217989901 39.0 34.0 41.0 11.0 41.0 22 33.81605457443164 39.0 33.0 41.0 10.0 41.0 23 33.68603379622964 39.0 33.0 41.0 2.0 41.0 24 33.54527078689062 39.0 33.0 41.0 2.0 41.0 25 33.39791249820021 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9656182821005999 1101 2 3.2603458967534387 1101 3 1.7638756502475985 1101 4 1.3283952618901083 1101 5 0.8965634462625189 1101 6 0.538080927987437 1101 7 0.2927602345277549 1101 8 0.19956075305007914 1101 9 0.2387608559701988 1101 10 0.06045988942664593 1101 11 0.2546490301690767 1101 12 -0.12187342755807862 1101 13 0.09728216574623616 1101 14 0.244460445521554 1101 15 0.2728486230773228 1101 16 0.03224572537839521 1101 17 0.055115959805633 1101 18 -0.05424131830353929 1101 19 -0.24440836524554044 1101 20 -0.1291953399666994 1101 21 0.13091328313159067 1101 22 0.05408559109844191 1101 23 0.017296441624914394 1101 24 -0.201742078341411 1101 25 -0.4567458241182223 1102 1 1.1652471667123372 1102 2 3.5008983730803607 1102 3 2.090686325121254 1102 4 1.2526209122666074 1102 5 1.0336859995621168 1102 6 0.7025093250680428 1102 7 0.5938979411697289 1102 8 0.5899044367723008 1102 9 0.6581353142912718 1102 10 0.6980045302020699 1102 11 0.7008368968826915 1102 12 0.6384431943271167 1102 13 0.6018391025328071 1102 14 0.6605036286882253 1102 15 0.8703902248229198 1102 16 0.8631880336582256 1102 17 0.7906906178343789 1102 18 0.5570587272015075 1102 19 0.5733185851603864 1102 20 0.9234261046497991 1102 21 0.7668491676420217 1102 22 0.6679788616573177 1102 23 0.40010807483813693 1102 24 0.37526798261605876 1102 25 0.3683174086055345 1103 1 1.0932749358473721 1103 2 3.0193769045053784 1103 3 1.7692422523147826 1103 4 1.1769756882466922 1103 5 1.0468654488464963 1103 6 0.9207747264370525 1103 7 0.7980703120471375 1103 8 0.629218375789101 1103 9 0.799752458037382 1103 10 0.6860719695300048 1103 11 0.7757956709959473 1103 12 0.6931911719251289 1103 13 0.677608393136417 1103 14 0.7839226548238756 1103 15 0.44628047062254694 1103 16 0.5313332576781349 1103 17 0.7050901200123505 1103 18 0.7001514465543437 1103 19 0.6047922161498107 1103 20 0.5647904481469936 1103 21 0.9251542392039411 1103 22 0.874004841744437 1103 23 0.887606519144299 1103 24 0.817678141296831 1103 25 0.9937370570187269 1104 1 1.6378537071369088 1104 2 4.051043571172045 1104 3 2.6493836756724463 1104 4 2.0412199096578902 1104 5 1.553478891668881 1104 6 1.2341993006462957 1104 7 1.342681017643244 1104 8 1.1822414901443352 1104 9 1.2824197329765568 1104 10 1.1267851690433872 1104 11 1.0522828972733222 1104 12 1.3208310746015286 1104 13 1.3006102179539312 1104 14 1.3329570222204694 1104 15 1.058632964539818 1104 16 1.264370058164758 1104 17 1.3982288061437345 1104 18 1.2674263857270915 1104 19 1.254389235614532 1104 20 1.3437624676117181 1104 21 1.3350052124399525 1104 22 1.425735377024239 1104 23 1.6043218476114518 1104 24 1.6531024113698223 1104 25 1.4443650983812546 1105 1 1.2059237453711802 1105 2 3.5069959521244236 1105 3 2.255879157076688 1105 4 1.6512911644371684 1105 5 1.2164785440845947 1105 6 0.9031556788180026 1105 7 0.6419988834757078 1105 8 0.5395755186462452 1105 9 0.6355262675611968 1105 10 0.7028279219109592 1105 11 0.3953790043292784 1105 12 0.29307212430607876 1105 13 0.36707267885070394 1105 14 0.5355595595857849 1105 15 0.41869118490826196 1105 16 0.4085654005352808 1105 17 0.695209167631397 1105 18 0.6614609703638692 1105 19 0.8436315018640954 1105 20 0.9181237814803325 1105 21 0.7977137630134621 1105 22 0.8864453179349141 1105 23 1.0647493762871534 1105 24 1.0255650460587376 1105 25 1.0384691998758697 1106 1 0.04129838845858913 1106 2 2.5676457633061496 1106 3 1.190309708794473 1106 4 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0.4359078459194592 1107 16 0.5405301104097262 1107 17 0.5327148231002354 1107 18 0.4790113040365256 1107 19 0.4076796864586001 1107 20 0.5599982866268078 1107 21 0.45142799217305907 1107 22 0.7831467657349336 1107 23 0.9030934550112804 1107 24 0.8577983907030102 1107 25 1.033765263669558 1108 1 0.3707880477354841 1108 2 2.231696251824726 1108 3 1.1708433595408891 1108 4 0.7235272616732686 1108 5 0.5591192367252873 1108 6 0.42283183599415963 1108 7 0.33318686216368576 1108 8 0.3935715226422474 1108 9 0.5944465139814383 1108 10 0.41845396253700073 1108 11 0.27652702297729803 1108 12 0.3783659970999551 1108 13 0.40801923229725645 1108 14 0.36194567347189377 1108 15 0.21702202073909405 1108 16 0.3188551108249911 1108 17 0.46282904775127776 1108 18 0.3734614698643668 1108 19 0.45477036300295737 1108 20 0.3710666719232236 1108 21 0.6716606494603496 1108 22 0.63202057601017 1108 23 0.6299841415219163 1108 24 0.6527961482898377 1108 25 0.5971563460630165 1109 1 1.0037036309974852 1109 2 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-2.3331555630270913 2316 3 -1.7480710593735296 2316 4 -2.3287954156494095 2316 5 -2.3813569537509025 2316 6 -2.470275057112733 2316 7 -2.4561071771303524 2316 8 -2.5503595462888207 2316 9 -2.6866057670708408 2316 10 -2.873254329171292 2316 11 -3.3649564935062166 2316 12 -3.084795841061883 2316 13 -3.0945615202834986 2316 14 -3.5944729079466846 2316 15 -3.7324451787281028 2316 16 -3.5689237769538984 2316 17 -3.4167497068274777 2316 18 -3.4777706313677363 2316 19 -3.7169745587419634 2316 20 -3.5377744869179395 2316 21 -3.7975784447787433 2316 22 -3.5600373660477693 2316 23 -3.9047311431933664 2316 24 -3.5901275946339055 2316 25 -3.972732098825432 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 89279.0 3 5.0 4 696.0 5 3726.0 6 4242.0 7 3915.0 8 3618.0 9 3380.0 10 3297.0 11 3210.0 12 3146.0 13 3150.0 14 3175.0 15 3426.0 16 3358.0 17 3706.0 18 4357.0 19 5467.0 20 6985.0 21 9028.0 22 11243.0 23 13310.0 24 15134.0 25 16833.0 26 19394.0 27 21432.0 28 24913.0 29 29644.0 30 36453.0 31 45007.0 32 56839.0 33 72567.0 34 93658.0 35 124926.0 36 173014.0 37 236982.0 38 458789.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.476031835085124 19.46907597821392 13.218920824375784 24.835971362325175 2 16.264157455427288 22.03074579574339 38.654767635430844 23.050329113398476 3 18.023786945231937 25.191273651651088 30.37254298381932 26.41239641929766 4 12.404611420315472 17.45915109749619 38.49987339446808 31.63636408772026 5 13.300097312487278 37.77319487818562 35.770717381698304 13.155990427628803 6 31.00346055120573 37.498448461618665 18.44201963130483 13.056071355870774 7 27.180966471876193 32.28385208501934 22.795201898586487 17.73997954451798 8 25.03326506547508 36.0883137839012 21.22329795817042 17.6551231924533 9 26.189471384667325 15.595319070765045 20.70292136058745 37.51228818398018 10 15.109129003589638 29.020842745999513 34.44520711175545 21.4248211386554 11 33.44601639417515 23.198105385451782 24.693105708171768 18.662772512201297 12 22.573952525407993 25.187549959535875 31.874928629234457 20.36356888582167 13 28.231978571393107 22.26712029511501 26.222053690675377 23.278847442816502 14 21.237621027976772 22.071046936605885 28.055900170110988 28.63543186530636 15 23.031346040225806 30.0668278611609 24.149508720886935 22.752317377726364 16 22.353572013723046 28.403206347157333 27.273624344009573 21.969597295110045 17 21.73011531661022 28.442757197125555 27.879548415164624 21.9475790710996 18 21.46441639814709 27.923594802718792 29.258910795231692 21.35307800390243 19 22.908797751874577 27.50018928838737 28.03056914363451 21.56044381610354 20 22.9909389933594 27.48315025134984 28.38738906473034 21.138521690560417 21 22.920083932072366 26.990764617207315 27.84051168526342 22.2486397654569 22 22.801191341363737 27.572266496534148 27.93303266382961 21.693509498272505 23 22.50241110560829 27.39140094855439 27.96255680217266 22.143631143664656 24 22.4937565785554 27.04468949218517 28.44058447361627 22.02096945564316 25 22.578993522633546 27.223557580417832 28.201772106177426 21.995676790771196 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 863.0 1 863.0 2 836.5 3 810.0 4 810.0 5 810.0 6 1702.0 7 2594.0 8 2594.0 9 2594.0 10 3650.0 11 4706.0 12 4706.0 13 4706.0 14 7191.5 15 9677.0 16 9677.0 17 9677.0 18 16483.5 19 23290.0 20 23290.0 21 23290.0 22 34921.5 23 46553.0 24 46553.0 25 46553.0 26 63631.0 27 80709.0 28 80709.0 29 80709.0 30 101094.5 31 121480.0 32 121480.0 33 121480.0 34 136629.0 35 151778.0 36 151778.0 37 151778.0 38 164216.5 39 176655.0 40 176655.0 41 176655.0 42 186642.5 43 196630.0 44 196630.0 45 196630.0 46 199887.0 47 203144.0 48 203144.0 49 203144.0 50 197591.5 51 192039.0 52 192039.0 53 192039.0 54 173099.0 55 154159.0 56 154159.0 57 154159.0 58 132307.5 59 110456.0 60 110456.0 61 110456.0 62 90134.5 63 69813.0 64 69813.0 65 69813.0 66 53998.0 67 38183.0 68 38183.0 69 38183.0 70 27857.5 71 17532.0 72 17532.0 73 17532.0 74 12180.5 75 6829.0 76 6829.0 77 6829.0 78 4603.5 79 2378.0 80 2378.0 81 2378.0 82 1565.5 83 753.0 84 753.0 85 753.0 86 475.0 87 197.0 88 197.0 89 197.0 90 127.5 91 58.0 92 58.0 93 58.0 94 35.5 95 13.0 96 13.0 97 13.0 98 9.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.337480698862536 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.4824614101373794E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.8618460576030345E-4 15 0.0 16 0.0 17 6.206153525343448E-5 18 0.0 19 3.723692115206069E-4 20 2.4824614101373794E-4 21 6.206153525343448E-5 22 6.826768877877793E-4 23 6.206153525343448E-4 24 4.964922820274759E-4 25 6.206153525343448E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1611304.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.6128267069752 #Duplication Level Percentage of deduplicated Percentage of total 1 86.70015223466395 68.15744043092005 2 9.546765519060784 15.00996446724103 3 2.091735112548338 4.9331162975897325 4 0.7035587125459603 2.212349565902326 5 0.3215124459572835 1.2637501099087827 6 0.1710570711025544 0.8068367932552707 7 0.10181137520341012 0.5602575996965158 8 0.0723793323486275 0.45519551288713744 9 0.04861162080738601 0.34393472302286043 >10 0.22151114800441654 2.903277097668852 >50 0.011833863840441985 0.6539867969708978 >100 0.007798512261426725 1.3239406014697228 >500 4.773943707913804E-4 0.21372948276416748 >1k 7.956572846523006E-4 1.162220520702662 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4417 0.27412580121442015 No Hit TATCAACGCAGAGTACTTTTTTTTT 3047 0.1891014979172149 No Hit GGTATCAACGCAGAGTACTTTTTTT 3000 0.18618460576030346 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.206153525343448E-5 2 0.0 0.0 0.0 0.0 6.206153525343448E-5 3 0.0 0.0 0.0 0.0 6.206153525343448E-5 4 0.0 0.0 0.0 0.0 6.206153525343448E-5 5 0.0 0.0 0.0 0.0 1.8618460576030345E-4 6 0.0 0.0 0.0 0.0 1.8618460576030345E-4 7 0.0 0.0 0.0 0.0 1.8618460576030345E-4 8 0.0 0.0 0.0 0.0 1.8618460576030345E-4 9 0.0 0.0 0.0 0.0 1.8618460576030345E-4 10 0.0 0.0 0.0 0.0 1.8618460576030345E-4 11 0.0 0.0 0.0 0.0 1.8618460576030345E-4 12 0.0 0.0 0.0 0.0 2.4824614101373794E-4 13 0.0 0.0 0.0 0.0 2.4824614101373794E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGAT 40 0.0054417048 14.185021 3 CTAGGAC 220 0.0 11.605927 3 TAGGACC 260 0.0 11.275273 4 GGCGAGG 430 0.0 11.2160635 19 GGTATCA 1010 0.0 11.059326 1 GACGTGA 475 0.0 10.551665 7 GTGCTAT 160 3.2105163E-9 10.50269 1 GGTATGC 95 1.1003071E-4 10.405142 1 GGACGTG 775 0.0 10.371843 6 GTCCTAC 520 0.0 10.265073 1 AGGACGT 805 0.0 9.985314 5 TTAAGAC 180 4.274625E-10 9.982053 3 GTCATAC 110 4.4226523E-5 9.884885 1 TGGCGAG 765 0.0 9.765722 18 GTATCAA 1945 0.0 9.656185 1 GTATTAA 215 4.9112714E-11 9.655004 1 ACGTGAA 530 0.0 9.635109 8 GTCCTAA 525 0.0 9.60246 1 CTAGGCC 90 0.001161081 9.456681 3 TAGGACG 860 0.0 9.45668 4 >>END_MODULE