FastQCFastQC Report
Thu 26 May 2016
SRR1512104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611304
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56700.35188890488697355No Hit
TATCAACGCAGAGTACTTTTTTTTT39820.24712903337917613No Hit
GGTATCAACGCAGAGTACTTTTTTT38480.23881278765521588No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17730.11003510200433934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT602.5759036E-514.2455786
GGTATCA10300.011.4850241
CTAAGAC1950.011.20791153
ACCGTGC1201.2805867E-711.0798938
TAGGACC3500.010.8598394
GGCGAGG3600.010.28879319
CTAGATA856.578182E-410.0613224
TAGGACG7200.010.0302694
TTAGACT2002.5465852E-119.9774794
GTAGGAC7050.09.9741083
TAGGACT2105.456968E-129.9548534
AATGCCG1356.4705637E-79.84848918
TTTAGAC1554.0456143E-89.80888753
TAAGACA2450.09.6962854
GTATCAA22250.09.6900471
TGGCGAG7450.09.68796618
GGACGTG7050.09.5644066
TTAGACA1804.1600288E-99.502364
TAAACTC1106.816545E-59.5023595
AGGACGT7100.09.5023595
Produced by FastQC (version 0.11.5)