FastQCFastQC Report
Thu 26 May 2016
SRR1512103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2941176
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54700.18598002975680478No Hit
GTCCTACAGTGGACATTTCTAAATT36290.12338601974176315No Hit
TATCAACGCAGAGTACTTTTTTTTT34500.117300018768003No Hit
GGTATCAACGCAGAGTACTTTTTTT34210.11631401861024297No Hit
GTCCTAAAGTGTGTATTTCTCATTT33220.11294801807168288No Hit
CTGTAGGACGTGGAATATGGCAAGA31810.10815401730464276No Hit
CTTTAGGACGTGAAATATGGCGAGG30620.10410801665728267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT550.0031782412.0348597
GGTATCA15050.011.369271
TCCAACG2750.011.34734618
GGACGTG20250.011.1136726
CGACTGT600.00608456111.0319546
AGGACGT20950.011.0131485
TGTAGGA22050.010.8101432
TAGGACC7650.010.7538394
GACGTGG11450.010.6534417
AGCCGGT803.938301E-410.6379577
GATATAC5300.010.2638381
GTCTAGA2900.010.2316641
GTAGGAC22400.010.1313873
GGCGAGG9850.010.0801319
TAGGACG22750.010.0586484
CCAACGT1606.666596E-910.0471319
GTCCTAA12150.010.0127131
CGTGCGC951.7235968E-49.95381410
GACGTGA10550.09.9489267
TTAGGAC12950.09.9305843
Produced by FastQC (version 0.11.5)