FastQCFastQC Report
Thu 26 May 2016
SRR1512103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2941176
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63910.21729403476704556No Hit
GGTATCAACGCAGAGTACTTTTTTT40570.13793802207008354No Hit
GTCCTACAGTGGACATTTCTAAATT40460.1375640220102435No Hit
TATCAACGCAGAGTACTTTTTTTTT40300.1370200219232035No Hit
GTCCTAAAGTGTGTATTTCTCATTT36960.12566402010624322No Hit
CTTTAGGACGTGAAATATGGCGAGG36500.12410001985600318No Hit
CTGTAGGACGTGGAATATGGCAAGA36320.12348801975808316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT1551.8189894E-1212.25322712
CGTGCGC1201.0086296E-811.87031510
TAGGACC8100.011.2620934
GGTATCA18050.011.0986471
GGCGAGG10550.010.62230219
GCCGGTT1803.6379788E-1110.55139111
GTCCTAC23600.010.3075661
GGACGTG20700.010.1391996
TTAGGAC15000.010.1358823
GACGTGA11900.010.0555387
CCAACGA2002.5465852E-119.97191119
AGGACGT21350.09.9697215
CTAATAC2400.09.8983233
TGGCGAG21850.09.82304518
TCCTACA25600.09.68798352
TAGGACA10400.09.6851284
GTAGGAC24350.09.6779993
TGTAGGA25200.09.6155142
TAGGACG23150.09.5229134
CGGTTTC2002.6739144E-109.49625113
Produced by FastQC (version 0.11.5)