FastQCFastQC Report
Thu 26 May 2016
SRR1512102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240932
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47600.21241162159315857No Hit
GGTATCAACGCAGAGTACTTTTTTT33000.14726015782718976No Hit
TATCAACGCAGAGTACTTTTTTTTT32410.1446273247024006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.011.5514471
CCCTATA1353.144669E-810.9901742
TTTATAC3200.010.933683
ACCGTGC1401.0371332E-710.1315948
GTCTAAG2251.8189894E-1210.11072351
TACACCG750.002751930210.0865655
GTATCAA25350.09.9884691
TATACTA1804.292815E-109.9814975
CTTAGAC1554.366484E-89.7611923
CTAGGAC2253.6379788E-129.6662923
GTCCTAA2700.09.5245951
TAGGAGT2107.2759576E-119.4561554
TCCAACG1201.7970713E-59.45594418
CGGTCAA800.0046932649.45594414
GTATTAA2457.2759576E-129.2853581
TAGGACT2503.6379788E-129.07790854
TTATACA4600.09.0450184
CTTACAC3050.08.9910983
TTAGACC950.00190250858.9584634
CTCAACG1000.00201491318.9020412
Produced by FastQC (version 0.11.5)