##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512102_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2240932 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.93373739140679 31.0 28.0 33.0 16.0 34.0 2 25.494568777633592 31.0 23.0 33.0 7.0 34.0 3 27.102516720721557 31.0 25.0 33.0 16.0 34.0 4 31.271461606153153 35.0 32.0 37.0 19.0 37.0 5 31.621470888005526 35.0 32.0 37.0 19.0 37.0 6 31.847357706525678 35.0 32.0 37.0 17.0 37.0 7 31.85516115616181 35.0 32.0 37.0 17.0 37.0 8 31.883829138947544 35.0 32.0 37.0 17.0 37.0 9 33.329205437737514 38.0 33.0 39.0 18.0 39.0 10 33.152686917764576 37.0 33.0 39.0 17.0 39.0 11 33.16691001779617 38.0 33.0 39.0 17.0 39.0 12 33.02942213329097 37.0 33.0 39.0 17.0 39.0 13 33.00500416790871 38.0 33.0 39.0 17.0 39.0 14 33.957757754362916 39.0 33.0 41.0 17.0 41.0 15 33.85623437034234 39.0 33.0 41.0 16.0 41.0 16 33.80179853739426 38.0 33.0 41.0 11.0 41.0 17 33.71794726479875 38.0 33.0 41.0 11.0 41.0 18 33.64743374631627 38.0 33.0 41.0 10.0 41.0 19 33.646817038625 39.0 33.0 41.0 10.0 41.0 20 33.55109793603733 39.0 33.0 41.0 10.0 41.0 21 33.46636934989549 39.0 33.0 41.0 2.0 41.0 22 33.32816658426048 39.0 33.0 40.0 2.0 41.0 23 33.1869079472291 38.0 33.0 40.0 2.0 41.0 24 33.030149062979156 38.0 33.0 40.0 2.0 41.0 25 32.8718939262771 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1199374709584298 1101 2 3.455258668868197 1101 3 1.8723840063265342 1101 4 1.2220318838682722 1101 5 1.0751136597475188 1101 6 0.7010494516261794 1101 7 0.47330552899294887 1101 8 0.4386044346406379 1101 9 0.4341623421799383 1101 10 0.31084191825239316 1101 11 0.5814311566757695 1101 12 0.44337572872894526 1101 13 0.45545636047276616 1101 14 0.5000230544343296 1101 15 0.8326104848294733 1101 16 0.577799835324214 1101 17 0.47975899603053307 1101 18 0.5248855972074793 1101 19 0.4780623038992502 1101 20 0.44089373896553496 1101 21 0.47765353321755555 1101 22 0.45167666546520024 1101 23 0.4975215373409725 1101 24 0.3835337435056161 1101 25 0.11361836590794638 1102 1 1.3985131798649775 1102 2 3.8201272114715863 1102 3 2.438182339934574 1102 4 1.6436301993328009 1102 5 1.2014205993845728 1102 6 0.9587709845227899 1102 7 0.8454359476097189 1102 8 0.9985112383291366 1102 9 0.9255115932490625 1102 10 0.9471265107069939 1102 11 0.9473582836509564 1102 12 0.9537194460274989 1102 13 1.1072760139828333 1102 14 0.8718621547206666 1102 15 0.9674161256723011 1102 16 1.1037725255084467 1102 17 0.9102725468048902 1102 18 0.8949384561519125 1102 19 0.9558658914589344 1102 20 1.073258311078959 1102 21 1.046403381489256 1102 22 1.1935136830810151 1102 23 0.9669241686610164 1102 24 0.8593159907198782 1102 25 0.9314453867052634 1103 1 1.024110031071583 1103 2 2.919119159799749 1103 3 1.9636294780708141 1103 4 1.4447131483780815 1103 5 1.0675282719139823 1103 6 0.9206929801743335 1103 7 0.8203743226715936 1103 8 0.666207845976345 1103 9 0.8584710931273634 1103 10 0.7131285754895487 1103 11 0.8381481936597623 1103 12 0.8651853599633128 1103 13 0.9216608018934025 1103 14 1.055803406429746 1103 15 0.8254055599970229 1103 16 0.7402948370847824 1103 17 0.9050554587690343 1103 18 0.8462559801770624 1103 19 0.8864408451891777 1103 20 1.0901918966294062 1103 21 1.1242794978031583 1103 22 0.9653405092455998 1103 23 1.0625404262099707 1103 24 1.2164478215318582 1103 25 1.1723200769470452 1104 1 1.7869728907552513 1104 2 4.205641520149577 1104 3 2.754074417634069 1104 4 2.22925894208829 1104 5 1.717192941041592 1104 6 1.496343038026044 1104 7 1.3837354146417447 1104 8 1.3968946611732918 1104 9 1.4046746700436046 1104 10 1.295307956801878 1104 11 1.3362078094665009 1104 12 1.317060696158542 1104 13 1.1475894243794258 1104 14 1.315674580839591 1104 15 1.2440853107723342 1104 16 1.2305939211864612 1104 17 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2316 5 -2.1836955671539258 2316 6 -2.445544976947396 2316 7 -2.2789713624724826 2316 8 -2.447381432534854 2316 9 -2.6553128745808436 2316 10 -2.908213284471433 2316 11 -3.3193929599869243 2316 12 -3.1412384231263033 2316 13 -3.2157288536480344 2316 14 -3.569817774218226 2316 15 -3.860779876318066 2316 16 -3.5272333647224343 2316 17 -3.4212972812859057 2316 18 -3.50086223554241 2316 19 -3.6655716291884026 2316 20 -3.7660612075588134 2316 21 -3.849376405848325 2316 22 -3.7876237332501574 2316 23 -3.7679647229694275 2316 24 -3.60231286304969 2316 25 -3.9201957081073004 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 137330.0 3 5.0 4 1171.0 5 5734.0 6 6464.0 7 5887.0 8 5386.0 9 5189.0 10 5125.0 11 4971.0 12 4843.0 13 4921.0 14 4873.0 15 4993.0 16 5321.0 17 5704.0 18 6565.0 19 8100.0 20 10348.0 21 13442.0 22 16332.0 23 19390.0 24 22231.0 25 24962.0 26 27876.0 27 31587.0 28 36103.0 29 42900.0 30 52229.0 31 64154.0 32 80686.0 33 103126.0 34 132440.0 35 173483.0 36 238141.0 37 323867.0 38 605053.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95345329532534 18.1033605660502 12.730239025548299 25.21294711307617 2 17.196887941239073 21.327675440725624 37.0371960850943 24.438240532941 3 18.566159080239828 24.219119544903638 29.653376363048945 27.561345011807585 4 13.35239088022305 16.498046348572824 36.34804625932425 33.80151651187988 5 14.430022865486325 36.90419878871827 34.46374097919973 14.202037366595684 6 32.97279881763481 35.54842360232261 17.567021221527472 13.911756358515118 7 28.758123852040136 31.40479050680699 21.681693152670405 18.155392488482473 8 26.86755719297682 34.339303197523165 20.094943063613997 18.698196545886024 9 27.048924286859215 15.113131500643481 19.391708449877104 38.4462357626202 10 16.42901257155505 27.882907647353868 32.75659413137034 22.93148564972074 11 34.94889626280494 22.268725690917886 23.29927904996671 19.483098996310463 12 23.516153100584937 24.28931355346793 30.235723350820106 21.958809995127027 13 29.095126492013147 21.02228001563635 25.510903499079845 24.371689993270657 14 22.73390759229369 20.484649810769433 26.527711646616098 30.253730950320783 15 24.432557525172562 28.08920574118269 23.124084086442608 24.354152647202145 16 24.12366818805747 27.074538629463095 25.18559242315251 23.616200759326922 17 23.274701452208927 27.109803916318707 26.271402376958502 23.34409225451386 18 23.329802064498164 26.756992180039376 27.0576260234581 22.855579732004365 19 24.379386021671397 26.33696962773425 26.553041019649914 22.73060333094444 20 24.533005968955717 26.112128546744923 26.557836753345043 22.79702873095432 21 24.60817606975675 25.771175360229904 26.25436760630631 23.36628096370703 22 24.625153285531454 25.63201833535929 26.308750528801617 23.43407785030764 23 24.401494745898553 25.66745027479834 26.297415878149828 23.63363910115328 24 24.152570924952865 25.96383189977353 26.244742684382565 23.63885449089104 25 24.20770652911669 25.842161137491516 26.337580228931635 23.612552104460153 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 749.0 1 749.0 2 742.5 3 736.0 4 736.0 5 736.0 6 1640.0 7 2544.0 8 2544.0 9 2544.0 10 3432.5 11 4321.0 12 4321.0 13 4321.0 14 6471.0 15 8621.0 16 8621.0 17 8621.0 18 14586.5 19 20552.0 20 20552.0 21 20552.0 22 30998.0 23 41444.0 24 41444.0 25 41444.0 26 59348.5 27 77253.0 28 77253.0 29 77253.0 30 99475.0 31 121697.0 32 121697.0 33 121697.0 34 143945.5 35 166194.0 36 166194.0 37 166194.0 38 191111.5 39 216029.0 40 216029.0 41 216029.0 42 238840.0 43 261651.0 44 261651.0 45 261651.0 46 276786.0 47 291921.0 48 291921.0 49 291921.0 50 295194.0 51 298467.0 52 298467.0 53 298467.0 54 280260.5 55 262054.0 56 262054.0 57 262054.0 58 230197.0 59 198340.0 60 198340.0 61 198340.0 62 166030.5 63 133721.0 64 133721.0 65 133721.0 66 104685.5 67 75650.0 68 75650.0 69 75650.0 70 55975.5 71 36301.0 72 36301.0 73 36301.0 74 25566.0 75 14831.0 76 14831.0 77 14831.0 78 10174.5 79 5518.0 80 5518.0 81 5518.0 82 3615.0 83 1712.0 84 1712.0 85 1712.0 86 1086.5 87 461.0 88 461.0 89 461.0 90 289.0 91 117.0 92 117.0 93 117.0 94 80.5 95 44.0 96 44.0 97 44.0 98 24.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.397991549944398 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 4.4624290250663563E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 6.693643537599535E-4 15 0.0 16 0.0 17 4.462429025066356E-5 18 0.0 19 2.677457415039814E-4 20 1.7849716100265425E-4 21 8.924858050132713E-5 22 7.13988644010617E-4 23 7.13988644010617E-4 24 3.1237003175464494E-4 25 4.462429025066356E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2240932.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.13853065757289 #Duplication Level Percentage of deduplicated Percentage of total 1 79.72267532502732 54.32185956738105 2 12.515710490975392 17.05604245981267 3 3.752939352622326 7.671593194040045 4 1.5669496037743404 4.270785744625984 5 0.7952998082713442 2.709528018392941 6 0.4447861932027077 1.8184246596964704 7 0.28793003664127464 1.3733390740242306 8 0.19884841587835536 1.0839391105229697 9 0.1425076148004323 0.8739233532015162 >10 0.5483064163109943 6.122005863680686 >50 0.01670572286205978 0.7678350883788098 >100 0.006218128933162994 0.8084710590915829 >500 5.284191530618007E-4 0.26482195778128337 >1k 5.944715471944658E-4 0.8574308493696164 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4760 0.21241162159315857 No Hit GGTATCAACGCAGAGTACTTTTTTT 3300 0.14726015782718976 No Hit TATCAACGCAGAGTACTTTTTTTTT 3241 0.1446273247024006 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1370 0.0 11.551447 1 CCCTATA 135 3.144669E-8 10.990174 2 TTTATAC 320 0.0 10.93368 3 ACCGTGC 140 1.0371332E-7 10.131594 8 GTCTAAG 225 1.8189894E-12 10.1107235 1 TACACCG 75 0.0027519302 10.086565 5 GTATCAA 2535 0.0 9.988469 1 TATACTA 180 4.292815E-10 9.981497 5 CTTAGAC 155 4.366484E-8 9.761192 3 CTAGGAC 225 3.6379788E-12 9.666292 3 GTCCTAA 270 0.0 9.524595 1 TAGGAGT 210 7.2759576E-11 9.456155 4 TCCAACG 120 1.7970713E-5 9.455944 18 CGGTCAA 80 0.004693264 9.455944 14 GTATTAA 245 7.2759576E-12 9.285358 1 TAGGACT 250 3.6379788E-12 9.0779085 4 TTATACA 460 0.0 9.045018 4 CTTACAC 305 0.0 8.991098 3 TTAGACC 95 0.0019025085 8.958463 4 CTCAACG 100 0.0020149131 8.902041 2 >>END_MODULE