FastQCFastQC Report
Thu 26 May 2016
SRR1512102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240932
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59040.26346180963991767No Hit
TATCAACGCAGAGTACTTTTTTTTT42810.1910365865630907No Hit
GGTATCAACGCAGAGTACTTTTTTT41770.18639566037702168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC1051.36788E-913.5742993
GGTATCA16550.013.0235271
TGTTCGG803.7841705E-410.68379810
GTCCTAC3900.010.2707661
CGTTCAT750.002655974410.12982410
TAAGACT2002.5465852E-119.9771094
GTTCTAA1553.8269718E-89.844791
GTCTAAG2251.8189894E-129.7490761
GTCCTAA2800.09.5371411
TACATCG800.00450888939.5020095
TCAATAC2106.730261E-119.5020093
TAATACA2002.6375346E-109.5020084
GACGCTC1304.2573884E-69.4969218
TCTTATA2451.8189894E-129.308092
GACGTGA2054.2564352E-109.2652887
ACTGTTC4100.09.2652888
GTATCAA31800.09.2072381
ACGCTCT1356.879085E-69.14497959
TAGGACG3950.09.1411724
GTATAGG2201.5643309E-109.1036341
Produced by FastQC (version 0.11.5)