FastQCFastQC Report
Thu 26 May 2016
SRR1512101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418676
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51340.21226489203183893No Hit
TATCAACGCAGAGTACTTTTTTTTT33980.14049008631168458No Hit
GGTATCAACGCAGAGTACTTTTTTT32990.13639693782879558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAG450.00884060513.1825421
ACTGCGT604.2551802E-412.6086188
GGTATCA14100.011.8502631
TAGGACC4650.011.3884284
CCACGCG600.006081627711.032549
CAGGACG1356.0881575E-810.5071814
GTCCTAA7850.010.2017761
GGCGAGG6550.09.96177119
GACTGCG1252.7266215E-69.83472257
GTATCAA27050.09.8320811
GTAGGAC13400.09.8093173
TTAGGAC7750.09.6394923
TGGCGAG12300.09.61022818
ATTATAC3250.09.6019473
TAGGACG13650.09.560384
GGACGTG12300.09.5333466
AGGACGT13200.09.5281035
TGTAGGA13450.09.4826092
CCTAGAC1804.5292836E-99.4564643
AGGACGG1002.8863482E-49.4564645
Produced by FastQC (version 0.11.5)