FastQCFastQC Report
Thu 26 May 2016
SRR1512101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418676
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58320.24112365608291475No Hit
TATCAACGCAGAGTACTTTTTTTTT39230.1621961767512474No Hit
GGTATCAACGCAGAGTACTTTTTTT38390.15872320228091732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.011.5759361
TTAGTAC1001.9248164E-611.4029663
TAGGACC4500.011.4029664
ACTGCGT752.0816765E-411.3958958
GTATTAG1900.011.0462781
GGCGAGG7050.010.91096319
TGGCGAG13500.010.90347918
ACCGTGC1252.2527638E-710.6361688
GGACGTG14650.010.5661596
GTAGGAC13300.010.5027323
TCCAACG1909.094947E-1210.49621918
TATGGCG13950.010.34731216
AGGACGT14900.010.3315475
GTCCTAG3050.010.3219321
TAGGACG15800.010.2241784
GTCTACG750.002557814810.1759651
GACGTGG7150.010.0942657
CTGTAGG14200.010.0102471
ATGGCGA14850.09.91204817
ATATGGC24350.09.78887915
Produced by FastQC (version 0.11.5)