FastQCFastQC Report
Thu 26 May 2016
SRR1512100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836767
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46000.25044003948241667No Hit
GGTATCAACGCAGAGTACTTTTTTT30000.16333046053201086No Hit
TATCAACGCAGAGTACTTTTTTTTT29370.15990052086083864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC655.652666E-412.1902351
GGTATCA13250.011.1379651
CCGTAGG650.00781341210.6664551
TTGGCGT803.9421523E-410.6363884
GTCTTAG2051.8189894E-1210.629291
GTATAAA2300.010.3351981
AACCGTG750.00275536210.084877
ACCGCCC750.00275536210.084878
TACACCG750.00275536210.084875
CGAATCA856.8911916E-410.0107189
GTCTAAT1302.4894216E-69.9045661
TAGGACG6250.09.8327494
TAGGACC1356.878963E-79.8047364
GTATCAA24800.09.7847531
TCTAGGA1957.912604E-109.6506022
ATAGGAC1107.204481E-59.4545683
GTAGGAC5100.09.4545663
CCGTCCA900.00116362049.4545669
TGTACCG900.00116362049.4545665
GTATAAG1603.687128E-79.285531
Produced by FastQC (version 0.11.5)