##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512100_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1836767 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.901054407009706 31.0 28.0 33.0 16.0 34.0 2 25.417855394832333 31.0 23.0 33.0 2.0 34.0 3 27.053501070086735 31.0 25.0 33.0 16.0 34.0 4 31.217860512520097 35.0 32.0 37.0 17.0 37.0 5 31.57782342561686 35.0 32.0 37.0 18.0 37.0 6 31.80296956554642 35.0 32.0 37.0 17.0 37.0 7 31.8102911256572 35.0 32.0 37.0 17.0 37.0 8 31.840908509353664 35.0 32.0 37.0 17.0 37.0 9 33.27237150928779 38.0 33.0 39.0 17.0 39.0 10 33.10464255945365 37.0 33.0 39.0 17.0 39.0 11 33.119956967868 38.0 33.0 39.0 17.0 39.0 12 32.99618514487684 37.0 33.0 39.0 17.0 39.0 13 32.97026677852988 38.0 33.0 39.0 17.0 39.0 14 33.92316717362627 39.0 33.0 41.0 16.0 41.0 15 33.823882397712936 39.0 33.0 41.0 13.0 41.0 16 33.77178052523809 38.0 33.0 41.0 11.0 41.0 17 33.68713505850225 38.0 33.0 41.0 10.0 41.0 18 33.62374814007438 38.0 33.0 41.0 10.0 41.0 19 33.62735719881727 39.0 33.0 41.0 10.0 41.0 20 33.52777951694472 39.0 33.0 41.0 8.0 41.0 21 33.43819221490804 39.0 33.0 41.0 2.0 41.0 22 33.30277982999477 39.0 33.0 40.0 2.0 41.0 23 33.16376764173137 38.0 33.0 40.0 2.0 41.0 24 33.008294465220686 38.0 33.0 40.0 2.0 41.0 25 32.85686916195685 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1026010380652629 1101 2 3.266500919025141 1101 3 2.0002980797845424 1101 4 1.2786838152447153 1101 5 0.9115077172480319 1101 6 0.5495356876301365 1101 7 0.6058138393112813 1101 8 0.7056817696601847 1101 9 0.6650220566029645 1101 10 0.3609865371897385 1101 11 0.5853495838578979 1101 12 0.2773913522614322 1101 13 0.5076399244519365 1101 14 0.5958210306239309 1101 15 0.519214376362136 1101 16 0.5154034746034526 1101 17 0.5093744818776926 1101 18 0.3653857960023217 1101 19 0.406923352516813 1101 20 0.5813162131402052 1101 21 0.6016801183474385 1101 22 0.535070512370801 1101 23 0.5881133237975007 1101 24 0.4759273041725862 1101 25 0.20493242812231927 1102 1 1.1706236671031647 1102 2 3.6562858272017067 1102 3 2.29493066131117 1102 4 1.4423967870285317 1102 5 1.0265966416001397 1102 6 0.9157864368411488 1102 7 0.813328265626339 1102 8 0.7462719827563582 1102 9 0.7836310085705378 1102 10 0.8040933576561997 1102 11 0.9387708740582497 1102 12 0.9250892434136304 1102 13 0.8140073921423152 1102 14 0.751309704980585 1102 15 0.6332862615152521 1102 16 0.7328192848604118 1102 17 0.48069377420449655 1102 18 0.6206326327980349 1102 19 0.7671783910240677 1102 20 0.7959896919806013 1102 21 0.7433236429057146 1102 22 1.0596459819381678 1102 23 0.8807998107613955 1102 24 1.0297380155471743 1102 25 0.7124784746816104 1103 1 1.3447533674770433 1103 2 3.1836958317952373 1103 3 2.212328513399484 1103 4 1.5111107728758064 1103 5 1.3025980603615146 1103 6 1.1737219064569793 1103 7 1.1087562489500797 1103 8 0.869323366339426 1103 9 1.1461245398327122 1103 10 1.02626324851623 1103 11 0.989346228312499 1103 12 0.9127390419378827 1103 13 1.02222516038416 1103 14 1.1522377144700116 1103 15 0.7765201071144787 1103 16 0.7147649649064647 1103 17 0.9894999795411294 1103 18 0.975421824627368 1103 19 1.1640652564336165 1103 20 1.2169460766975178 1103 21 1.2056799497070472 1103 22 1.2716781250237403 1103 23 1.4253919459391184 1103 24 1.2888247751310828 1103 25 0.8942194809555559 1104 1 1.4630915439894636 1104 2 3.9956820869755987 1104 3 2.574909743934807 1104 4 1.9028841120945224 1104 5 1.6880489051590821 1104 6 1.2356342878297433 1104 7 1.2302489290059881 1104 8 1.1929706480285311 1104 9 1.2760403666062956 1104 10 1.3707656271915312 1104 11 1.1055782349826018 1104 12 1.1913269793566528 1104 13 1.0792447047574854 1104 14 1.3112828112109796 1104 15 1.0428603189882324 1104 16 1.2413947866284332 1104 17 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2316 3 -1.5870973229359286 2316 4 -2.1191691539712707 2316 5 -2.069166294281615 2316 6 -2.2700688091224315 2316 7 -2.2839311061394127 2316 8 -2.511226251240217 2316 9 -2.592452301987702 2316 10 -2.8603600176131856 2316 11 -3.0399355302990365 2316 12 -2.8951786514654856 2316 13 -3.074665693368093 2316 14 -3.4263718946824895 2316 15 -3.452018797841877 2316 16 -3.3808949078333335 2316 17 -3.2203550750183894 2316 18 -3.164985752156362 2316 19 -3.3902953504091364 2316 20 -3.4549254188855834 2316 21 -3.620615687222678 2316 22 -3.334356476278863 2316 23 -3.550851002944281 2316 24 -3.380675206852768 2316 25 -3.7272840071916526 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 114451.0 3 1.0 4 914.0 5 4665.0 6 5348.0 7 4855.0 8 4426.0 9 4322.0 10 4266.0 11 4006.0 12 3976.0 13 3972.0 14 4027.0 15 4135.0 16 4306.0 17 4655.0 18 5399.0 19 6607.0 20 8543.0 21 10862.0 22 13583.0 23 16284.0 24 18413.0 25 20870.0 26 23101.0 27 25832.0 28 29218.0 29 34972.0 30 42908.0 31 52923.0 32 66006.0 33 83719.0 34 107845.0 35 141295.0 36 193693.0 37 263907.0 38 498462.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.723673171392996 18.249511233596856 13.022337618217225 25.00447797679292 2 16.844090654770085 21.514737930558542 37.42666110773025 24.214510306941122 3 18.546554897817742 24.325404365387662 29.792728201236194 27.335312535558405 4 13.254321315659526 16.821894121573393 36.5817765671966 33.34200799557048 5 14.291088635629887 37.01345897438271 34.58800163548234 14.107450754505063 6 32.630758283440414 35.83524747559162 17.767141940158986 13.766852300808976 7 28.450260702636754 31.66868742742003 21.664478945886987 18.216572924056234 8 26.460626656786967 34.62651414472091 20.502917360595788 18.409941837896344 9 26.882832716397886 15.376637319812476 19.75449254042565 37.98603742336399 10 16.280344757936092 28.219365874931334 32.862306432987964 22.637982934144613 11 34.278925960668936 22.653063780000405 23.737088046551357 19.330922212779303 12 23.400082862986977 24.45606873381327 30.534629596459435 21.609218806740323 13 28.81279988153097 21.32349938778299 25.66901517721083 24.19468555347521 14 22.400234760954334 21.021830808781978 26.77772079343976 29.80021363682393 15 24.033097284522206 28.510584086059907 23.49693782608246 23.959380803335424 16 23.756905475762576 27.40913790371887 25.699721303790845 23.134235316727707 17 22.817725729747682 27.436498408887367 26.729058970526985 23.01671689083797 18 22.887116329942774 27.061189579298844 27.55118096089488 22.500513129863506 19 24.069393932794704 26.553496374049956 26.889359524379884 22.487750168775452 20 24.209819122009755 26.480553016366294 27.020306920381127 22.289320941242828 21 24.139029292821508 26.097201382431273 26.720416994235514 23.043352330511706 22 24.23108835596749 26.02136815422006 26.69130073085534 23.056242758957115 23 24.002942146852547 26.065221581115384 26.691489442788747 23.24034682924333 24 23.837557172777892 26.12893267862869 26.85336165861897 23.18014848997445 25 24.0246847038135 26.018026258122294 26.805497709287796 23.151791328776408 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1228.0 1 1228.0 2 1022.5 3 817.0 4 817.0 5 817.0 6 1589.5 7 2362.0 8 2362.0 9 2362.0 10 3059.5 11 3757.0 12 3757.0 13 3757.0 14 5650.0 15 7543.0 16 7543.0 17 7543.0 18 12904.5 19 18266.0 20 18266.0 21 18266.0 22 27566.5 23 36867.0 24 36867.0 25 36867.0 26 52716.0 27 68565.0 28 68565.0 29 68565.0 30 89045.5 31 109526.0 32 109526.0 33 109526.0 34 127045.0 35 144564.0 36 144564.0 37 144564.0 38 163290.5 39 182017.0 40 182017.0 41 182017.0 42 200365.0 43 218713.0 44 218713.0 45 218713.0 46 228107.0 47 237501.0 48 237501.0 49 237501.0 50 239042.0 51 240583.0 52 240583.0 53 240583.0 54 223937.5 55 207292.0 56 207292.0 57 207292.0 58 181617.0 59 155942.0 60 155942.0 61 155942.0 62 128873.0 63 101804.0 64 101804.0 65 101804.0 66 79154.5 67 56505.0 68 56505.0 69 56505.0 70 41793.0 71 27081.0 72 27081.0 73 27081.0 74 18796.5 75 10512.0 76 10512.0 77 10512.0 78 7133.5 79 3755.0 80 3755.0 81 3755.0 82 2443.0 83 1131.0 84 1131.0 85 1131.0 86 729.0 87 327.0 88 327.0 89 327.0 90 208.5 91 90.0 92 90.0 93 90.0 94 53.0 95 16.0 96 16.0 97 16.0 98 9.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.5424378813425985 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.177739473760145E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.722174342200181E-4 15 0.0 16 0.0 17 1.0888697368800724E-4 18 0.0 19 3.8110440790802534E-4 20 2.177739473760145E-4 21 1.6333046053201085E-4 22 8.166523026600543E-4 23 7.07765328972047E-4 24 5.444348684400362E-4 25 1.0888697368800724E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1836767.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.43082704155108 #Duplication Level Percentage of deduplicated Percentage of total 1 79.49723037571258 54.400612221227284 2 12.944043553526626 17.7154321125937 3 3.807584223179177 7.816684122675387 4 1.5011616821730778 4.10902941736759 5 0.7668526680529465 2.623818114694158 6 0.42065866303034316 1.7271612128015719 7 0.24950151147926344 1.195151634505013 8 0.16729132923180612 0.9158307212970321 9 0.12749860502500632 0.785235148965472 >10 0.49192145116465313 5.549446888203297 >50 0.01708806289340188 0.7810681734001775 >100 0.007318752919092504 0.9408701652412804 >500 0.0012863454691989747 0.5938982888859602 >1k 5.627761427745493E-4 0.845761778142088 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4600 0.25044003948241667 No Hit GGTATCAACGCAGAGTACTTTTTTT 3000 0.16333046053201086 No Hit TATCAACGCAGAGTACTTTTTTTTT 2937 0.15990052086083864 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 5.444348684400362E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAC 65 5.652666E-4 12.190235 1 GGTATCA 1325 0.0 11.137965 1 CCGTAGG 65 0.007813412 10.666455 1 TTGGCGT 80 3.9421523E-4 10.636388 4 GTCTTAG 205 1.8189894E-12 10.62929 1 GTATAAA 230 0.0 10.335198 1 AACCGTG 75 0.002755362 10.08487 7 ACCGCCC 75 0.002755362 10.08487 8 TACACCG 75 0.002755362 10.08487 5 CGAATCA 85 6.8911916E-4 10.010718 9 GTCTAAT 130 2.4894216E-6 9.904566 1 TAGGACG 625 0.0 9.832749 4 TAGGACC 135 6.878963E-7 9.804736 4 GTATCAA 2480 0.0 9.784753 1 TCTAGGA 195 7.912604E-10 9.650602 2 ATAGGAC 110 7.204481E-5 9.454568 3 GTAGGAC 510 0.0 9.454566 3 CCGTCCA 90 0.0011636204 9.454566 9 TGTACCG 90 0.0011636204 9.454566 5 GTATAAG 160 3.687128E-7 9.28553 1 >>END_MODULE