FastQCFastQC Report
Thu 26 May 2016
SRR1512100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836767
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61110.33270414810370613No Hit
TATCAACGCAGAGTACTTTTTTTTT41150.22403494836307492No Hit
GGTATCAACGCAGAGTACTTTTTTT40920.22278274816566282No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18910.10295263362201085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAA1800.012.1899221
GGTATCA13650.012.0909131
CTATTAG909.1199596E-510.5999331
TAGGACA2800.010.5197874
AAAGCCG750.002643934710.1351935
GTCCTAA3600.010.0699361
CTAAGAC2105.456968E-129.9542073
GTATTAG1252.435745E-69.9215361
ACTAGAC1151.0242278E-59.9148633
GTATCAA27850.09.8653591
GTCCTAT1606.218215E-89.5399391
GTATAAT1502.4912515E-79.5399391
TTAGGAC3900.09.5017433
TCCTATA1901.0477379E-99.5017432
ACCGACC800.00452958059.496578
GTGTAGA3550.09.4055741
TATACAG3300.09.2138115
TAAACAG3200.09.2048145
TGCTATA1451.7017501E-69.1740972
CTACGAG1252.7478942E-59.1216743
Produced by FastQC (version 0.11.5)