FastQCFastQC Report
Thu 26 May 2016
SRR1512099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722784
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45580.2645717629139811No Hit
TATCAACGCAGAGTACTTTTTTTTT30070.17454306517822316No Hit
GGTATCAACGCAGAGTACTTTTTTT29800.17297583446328732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG350.002239934416.2089185
GTATACG450.008772870513.1975811
GGTATCA10400.012.7534311
TAGGACC3400.011.1237674
CTAGGAC1701.2732926E-1111.1237673
AACACCG600.006086459411.031075
ACCGTGC803.939437E-410.6371038
GTATCAA21250.010.6201721
GTACTAG951.085529E-410.4191441
CTCAACG1052.6121199E-510.369532
GACTGTC1752.564775E-1010.2656487
GACAGCG1201.6188042E-610.2431367
TGGACTG3200.010.0461535
GTCCTAC8050.09.9596661
GTAGGAC8450.09.9587343
GTATTAA2102.910383E-119.8981871
GTTCTAA1406.040391E-79.8981861
GTCTTGG2400.09.8981861
ATTATCC1252.7295682E-69.833413
GGACGTG6800.09.7332966
Produced by FastQC (version 0.11.5)