FastQCFastQC Report
Thu 26 May 2016
SRR1512099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722784
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56370.32720294592937943No Hit
GGTATCAACGCAGAGTACTTTTTTT37760.21918011776287682No Hit
TATCAACGCAGAGTACTTTTTTTTT37350.21680024889945576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12650.013.9567521
CCGTGCA701.094992E-412.2095479
AGGACCG550.003063589612.0935895
TCCAACG752.0815521E-411.39557718
GGCGAGG4500.011.39557719
CTACGCT600.005866409311.0857914
TACACCG600.005866409311.0857915
ATTACAC1803.6379788E-1211.085793
TAGGACC3600.011.085794
TGGCGAG8850.010.51569218
GTCTTAG2001.8189894E-1210.4977411
CTAATAC1752.3464963E-1010.3165713
TTATACT2151.8189894E-1210.1650434
GATCTAA951.5701339E-410.0456861
TAGGACA5300.09.8606764
TAGAAAT3850.09.6255094
GTAGGAC9000.09.6076853
GTATCAA25900.09.5065541
TAAGGTG1304.2258016E-69.5021065
CCAGTAC1804.1636667E-99.5021063
Produced by FastQC (version 0.11.5)