##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512099_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1722784 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.365048084960158 31.0 31.0 34.0 28.0 34.0 2 31.54717538588703 33.0 31.0 34.0 28.0 34.0 3 31.650264339580588 34.0 31.0 34.0 28.0 34.0 4 35.23771407210654 37.0 35.0 37.0 32.0 37.0 5 34.92154791314523 37.0 35.0 37.0 32.0 37.0 6 34.89178562141278 37.0 35.0 37.0 32.0 37.0 7 34.752523241451044 37.0 35.0 37.0 32.0 37.0 8 34.67004685439382 37.0 35.0 37.0 32.0 37.0 9 36.24348496387243 39.0 37.0 39.0 32.0 39.0 10 36.08285658561955 39.0 37.0 39.0 31.0 39.0 11 36.08333255939224 39.0 37.0 39.0 31.0 39.0 12 35.936079624607615 39.0 35.0 39.0 30.0 39.0 13 35.9095307362966 39.0 35.0 39.0 30.0 39.0 14 37.022017850177384 40.0 37.0 41.0 31.0 41.0 15 36.966277258205324 40.0 37.0 41.0 31.0 41.0 16 36.87737232293776 40.0 36.0 41.0 31.0 41.0 17 36.8255573536787 40.0 36.0 41.0 31.0 41.0 18 36.7643384196742 40.0 36.0 41.0 30.0 41.0 19 36.78310978044839 40.0 37.0 41.0 30.0 41.0 20 36.69004007466984 40.0 36.0 41.0 30.0 41.0 21 36.586090885450524 40.0 36.0 41.0 30.0 41.0 22 36.49292424354997 40.0 36.0 41.0 30.0 41.0 23 36.560776626669394 40.0 37.0 41.0 30.0 41.0 24 36.46612343741293 40.0 36.0 41.0 30.0 41.0 25 36.07387577316715 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07719743397943546 1101 2 0.0054587086696002984 1101 3 -0.02505879064436556 1101 4 -0.06103657956386144 1101 5 0.05202844103202864 1101 6 0.03948609292263683 1101 7 0.09029469271006008 1101 8 -0.029127751695973814 1101 9 -0.012557000108230909 1101 10 0.0989173988526062 1101 11 -0.09567470934711508 1101 12 -0.02502345924798277 1101 13 0.04287953940795575 1101 14 -0.07299307685659073 1101 15 -0.0019737785407087927 1101 16 -0.05713306734649137 1101 17 -0.09940419090549568 1101 18 -0.10898295176684059 1101 19 -0.25212369443153904 1101 20 -0.13184756318059243 1101 21 -0.3760966878398406 1101 22 -0.420579704203881 1101 23 -0.37055978563626013 1101 24 -0.5246782133099046 1101 25 -0.4600320689240931 1102 1 0.19221360879953053 1102 2 0.17980016242282204 1102 3 0.19886673916351683 1102 4 0.20053006729690281 1102 5 0.14775036616571668 1102 6 0.2226345711691522 1102 7 0.32530162477581825 1102 8 0.2135506664726421 1102 9 0.37622955828616966 1102 10 0.36536950157921666 1102 11 0.23478016620580178 1102 12 0.26514621131384075 1102 13 0.6224233434615627 1102 14 0.48115625964568665 1102 15 0.4362351077073896 1102 16 0.5032353224336674 1102 17 0.49080509146065054 1102 18 0.45881265132288007 1102 19 0.2520322440381264 1102 20 0.47728625869832086 1102 21 0.5253953567894598 1102 22 0.5919904416996005 1102 23 0.5677382271715601 1102 24 0.5900408729778093 1102 25 0.4682936721467428 1103 1 0.15028719040252625 1103 2 0.18719044040133426 1103 3 0.2634296978441242 1103 4 0.2306050620777782 1103 5 0.30157799058157764 1103 6 0.2737370105068848 1103 7 0.22007447248984136 1103 8 0.24913384323228627 1103 9 0.3236625527779893 1103 10 0.3091442924128316 1103 11 0.2701577898187182 1103 12 0.46693516604397445 1103 13 0.307560887422639 1103 14 0.3457256418621242 1103 15 0.3021470089134084 1103 16 0.4873113770979529 1103 17 0.36362550545753436 1103 18 0.3775035347196436 1103 19 0.30261437697319593 1103 20 0.4643653163656154 1103 21 0.4543671092906223 1103 22 0.4485560982652501 1103 23 0.39106850932537185 1103 24 0.46222535692700006 1103 25 0.4701147445560494 1104 1 0.30852100561308404 1104 2 0.29122528920362356 1104 3 0.2902297537139482 1104 4 0.2966938252595668 1104 5 0.2690827045979205 1104 6 0.3497932993538697 1104 7 0.27315429649989653 1104 8 0.31913523997786797 1104 9 0.3973984980255594 1104 10 0.17104631071019583 1104 11 0.4085386125018644 1104 12 0.6103914129885908 1104 13 0.46599897667468326 1104 14 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0.2449479303485731 1107 12 0.33054170918332915 1107 13 0.21179081256468635 1107 14 0.36394508488690747 1107 15 0.3625581430731515 1107 16 0.3737407430601323 1107 17 0.34180346094360914 1107 18 0.4351648667969954 1107 19 0.37283888401639587 1107 20 0.5010483843056406 1107 21 0.27539948760887256 1107 22 0.4001442826676751 1107 23 0.2617449699907084 1107 24 0.32608944348297086 1107 25 0.2140701571432757 1108 1 -0.2557165232347778 1108 2 -0.22020670592711156 1108 3 -0.15718852124737737 1108 4 -0.10467121747331021 1108 5 -0.05858588103267692 1108 6 -0.16912962930631892 1108 7 -0.15674476768818124 1108 8 -0.1252093112635393 1108 9 -0.32838895347199326 1108 10 -0.28203565525566887 1108 11 -0.12592094977146928 1108 12 -0.10746889996314479 1108 13 -0.2564345147407039 1108 14 -0.3022470854999142 1108 15 -0.20114646995259022 1108 16 -0.14769407546474866 1108 17 -0.0028682511125595056 1108 18 -0.08522189518135548 1108 19 -0.13631462836801944 1108 20 -0.08828024086009378 1108 21 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2314 15 -0.5395417923498584 2314 16 -0.4907028072992077 2314 17 -0.4499126527614763 2314 18 -0.5891585681126728 2314 19 -0.5269501419530229 2314 20 -0.6571449476978373 2314 21 -0.4752448735128496 2314 22 -0.522129480930964 2314 23 -0.3719744728665475 2314 24 -0.4830049797926961 2314 25 -0.5024182747854766 2315 1 -0.4740879211234237 2315 2 -0.4872382798374417 2315 3 -0.6072456499261953 2315 4 -0.5665093707908397 2315 5 -0.6678124257362157 2315 6 -0.6438647964804005 2315 7 -0.5749264197177553 2315 8 -0.6859570628083418 2315 9 -0.7956037933039966 2315 10 -0.8541473893146048 2315 11 -0.8235255329148856 2315 12 -0.7573096984243506 2315 13 -0.707175636811094 2315 14 -1.1481336821647972 2315 15 -1.0152797118652614 2315 16 -1.0432117310787845 2315 17 -0.9105366201012401 2315 18 -1.0127017035302828 2315 19 -0.856841574661523 2315 20 -0.8073245553242572 2315 21 -0.9487048300941652 2315 22 -1.1168296023159812 2315 23 -0.91789032253849 2315 24 -0.8471672250912761 2315 25 -0.8848798640698448 2316 1 -0.9085114615501624 2316 2 -0.7886959594459455 2316 3 -0.879210742106828 2316 4 -0.7977068684346378 2316 5 -1.0393326021627587 2316 6 -1.0409072180737766 2316 7 -0.9823440829849517 2316 8 -1.1555079623875102 2316 9 -1.3938407696153092 2316 10 -1.5620790091283183 2316 11 -1.4995625417598006 2316 12 -1.4204353340404623 2316 13 -1.527290100484585 2316 14 -1.8392431900504675 2316 15 -1.7592302607768175 2316 16 -1.787994246373927 2316 17 -1.7251429696190073 2316 18 -1.7240332098488835 2316 19 -1.5037235012473218 2316 20 -1.7115254888650426 2316 21 -1.741628397218328 2316 22 -1.6084392834245023 2316 23 -1.6646405004178675 2316 24 -1.486181729866395 2316 25 -1.8680175197375846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15376.0 3 0.0 4 272.0 5 2518.0 6 3271.0 7 2810.0 8 2309.0 9 2116.0 10 1994.0 11 2044.0 12 1891.0 13 1885.0 14 1909.0 15 1851.0 16 1933.0 17 2236.0 18 2364.0 19 2565.0 20 3315.0 21 4076.0 22 5320.0 23 6929.0 24 8655.0 25 10439.0 26 12578.0 27 14551.0 28 17174.0 29 21468.0 30 28087.0 31 36600.0 32 48051.0 33 63592.0 34 83734.0 35 113601.0 36 164884.0 37 285893.0 38 744493.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.20559548628829 19.590850111530667 13.506086804391245 24.697467597789796 2 15.262969704849825 21.040768894997864 39.994276705611384 23.70198469454093 3 18.15712242509798 25.204088266433867 30.61672270000186 26.0220666084663 4 12.856515964856882 17.639529970094916 38.086144287386 31.417809777662203 5 13.374668567893849 37.75779101408786 35.66020694722352 13.207333470794769 6 31.335268960008918 37.48966788639783 18.18666762635362 12.98839552723963 7 27.614082786930922 32.07929723052919 22.64648383082267 17.66013615171722 8 25.234657710298002 36.24455948202902 20.85240683058555 17.668375977087422 9 26.383342311050022 15.53706094321749 20.281126362910264 37.798470382822224 10 15.276088006389656 28.810576369411372 34.21009250143953 21.703243122759442 11 33.92955820346602 22.89126205026283 24.541846221000426 18.637333525270723 12 22.98529589315898 24.999651726507793 31.60790905882572 20.40714332150751 13 28.683804818247673 21.894096996489402 26.13717099764103 23.284927187621896 14 21.485572190129464 21.69337537381355 27.813759589130154 29.007292846926834 15 23.20314096253506 29.844948641269013 23.88546677935249 23.066443616843436 16 22.60997315972287 28.082742816278767 26.948241915411337 22.359042108587033 17 22.04240345858796 28.18229098946821 27.58865882199974 22.18664672994409 18 21.8765091851329 27.536649980496687 28.962307520849972 21.62453331352044 19 23.385663421140922 26.98559655255279 27.849664089826803 21.779075936479487 20 23.498767111837584 27.038909114549476 28.068521648680278 21.393802124932666 21 23.286552463918866 26.61372522614559 27.466705054144917 22.633017255790627 22 23.36143126474358 27.12795103739064 27.61715920277876 21.893458495087025 23 22.882090848301353 27.094981146794954 27.651580232925312 22.371347771978378 24 22.933931312155625 26.750341906736665 28.104501559187593 22.21122522192011 25 22.94199185259915 26.974189061957627 27.885518453549484 22.19830063189374 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 930.0 1 930.0 2 826.0 3 722.0 4 722.0 5 722.0 6 1474.5 7 2227.0 8 2227.0 9 2227.0 10 3117.0 11 4007.0 12 4007.0 13 4007.0 14 6509.0 15 9011.0 16 9011.0 17 9011.0 18 15651.0 19 22291.0 20 22291.0 21 22291.0 22 33913.5 23 45536.0 24 45536.0 25 45536.0 26 63332.5 27 81129.0 28 81129.0 29 81129.0 30 102237.5 31 123346.0 32 123346.0 33 123346.0 34 140448.0 35 157550.0 36 157550.0 37 157550.0 38 171497.5 39 185445.0 40 185445.0 41 185445.0 42 197763.0 43 210081.0 44 210081.0 45 210081.0 46 215475.5 47 220870.0 48 220870.0 49 220870.0 50 217107.5 51 213345.0 52 213345.0 53 213345.0 54 193623.0 55 173901.0 56 173901.0 57 173901.0 58 148680.0 59 123459.0 60 123459.0 61 123459.0 62 100606.0 63 77753.0 64 77753.0 65 77753.0 66 59908.5 67 42064.0 68 42064.0 69 42064.0 70 30431.5 71 18799.0 72 18799.0 73 18799.0 74 12912.5 75 7026.0 76 7026.0 77 7026.0 78 4699.0 79 2372.0 80 2372.0 81 2372.0 82 1541.0 83 710.0 84 710.0 85 710.0 86 434.0 87 158.0 88 158.0 89 158.0 90 102.5 91 47.0 92 47.0 93 47.0 94 26.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4648870665155934 2 0.0 3 0.0 4 0.0 5 0.06321163883574493 6 0.0 7 0.0 8 0.003598826086148931 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.1609116406932036E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005340193547188736 25 0.0015091851329011646 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1722784.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.1234949899148 #Duplication Level Percentage of deduplicated Percentage of total 1 81.98664162069932 59.13163136170447 2 12.221897464903638 17.6297192115446 3 3.1008469341180804 6.70931754952074 4 1.1285939968374081 3.2559257390620293 5 0.5338309398769604 1.925087655883873 6 0.2902176378380791 1.2558906209159764 7 0.17148907849920922 0.8657874185773975 8 0.11693027289304159 0.6746735960936524 9 0.07676713025268361 0.49830423607526086 >10 0.34712956437308895 4.145644889706237 >50 0.014393202028599561 0.7107771404992078 >100 0.009331907163832781 1.3488526579258353 >500 0.0010455523629156079 0.49909084214999655 >1k 8.042710483966208E-4 1.0223125292946924 >5k 8.042710483966207E-5 0.3269845510459703 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5637 0.32720294592937943 No Hit GGTATCAACGCAGAGTACTTTTTTT 3776 0.21918011776287682 No Hit TATCAACGCAGAGTACTTTTTTTTT 3735 0.21680024889945576 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 5.804558203466018E-5 10 0.0 0.0 0.0 0.0 5.804558203466018E-5 11 0.0 0.0 0.0 0.0 5.804558203466018E-5 12 0.0 5.804558203466018E-5 0.0 0.0 5.804558203466018E-5 13 0.0 5.804558203466018E-5 0.0 5.804558203466018E-5 5.804558203466018E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1265 0.0 13.956752 1 CCGTGCA 70 1.094992E-4 12.209547 9 AGGACCG 55 0.0030635896 12.093589 5 TCCAACG 75 2.0815521E-4 11.395577 18 GGCGAGG 450 0.0 11.395577 19 CTACGCT 60 0.0058664093 11.085791 4 TACACCG 60 0.0058664093 11.085791 5 ATTACAC 180 3.6379788E-12 11.08579 3 TAGGACC 360 0.0 11.08579 4 TGGCGAG 885 0.0 10.515692 18 GTCTTAG 200 1.8189894E-12 10.497741 1 CTAATAC 175 2.3464963E-10 10.316571 3 TTATACT 215 1.8189894E-12 10.165043 4 GATCTAA 95 1.5701339E-4 10.045686 1 TAGGACA 530 0.0 9.860676 4 TAGAAAT 385 0.0 9.625509 4 GTAGGAC 900 0.0 9.607685 3 GTATCAA 2590 0.0 9.506554 1 TAAGGTG 130 4.2258016E-6 9.502106 5 CCAGTAC 180 4.1636667E-9 9.502106 3 >>END_MODULE