FastQCFastQC Report
Thu 26 May 2016
SRR1512098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2063763
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54150.26238477964766305No Hit
TATCAACGCAGAGTACTTTTTTTTT37650.1824337387577934No Hit
GGTATCAACGCAGAGTACTTTTTTT37310.18078626276369913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.013.5712781
GTACCGT752.1629574E-411.3488166
GGACGTG12800.011.1567146
TAGGACC4250.011.126294
CGTGAAA7250.011.0879249
GACGTGA6850.011.0450777
TTAGGAC7900.011.0136183
GTCCTAA5750.010.9944521
AGGACGT12850.010.8925095
ACGTGAA7600.010.8261748
TAGGACG13700.010.768954
GGCGAGG6300.010.50841819
CTTATAC1803.8198777E-1110.5081643
GTAGGAC13150.010.3563353
TGGCGAG11600.010.35441618
TAGAACC1651.0059011E-910.3171064
TCTATAC3050.010.2325393
CCGTGTA750.002748898210.0878369
ACAGCGT951.7207825E-49.9551028
TAAGACC1901.09139364E-109.9551024
Produced by FastQC (version 0.11.5)