FastQCFastQC Report
Thu 26 May 2016
SRR1512098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2063763
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63170.3060913486674584No Hit
TATCAACGCAGAGTACTTTTTTTTT45720.22153706602938417No Hit
GGTATCAACGCAGAGTACTTTTTTT43180.20922945124997394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.012.1514931
TACGATG550.003064723212.0931785
TAGGACC4550.011.69454
GGCGAGG6600.010.93614419
GTTCTAA2300.010.7871681
CAATGCG750.00265687110.1293419
TGGCGAG13050.09.89720218
TTAGGAC9800.09.8896093
GTCCTAG2900.09.8715481
TAAGACA3000.09.8185084
GTATCAA25050.09.7139181
GACGTGA7850.09.6781847
GTCCTAA8400.09.6560961
GCACCGT800.00452940459.4967176
ACAGCGC900.00111736549.4967178
ATTATAC2550.09.3154733
TATGGCG14100.09.29420916
AGGACGT13300.09.2160135
ATGGCGA14350.09.19846517
CGTGAAA8700.09.1687999
Produced by FastQC (version 0.11.5)