##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512098_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2063763 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.263824867487205 31.0 31.0 34.0 28.0 34.0 2 31.452702660140723 33.0 31.0 34.0 28.0 34.0 3 31.552012028512966 33.0 31.0 34.0 28.0 34.0 4 35.13520350931769 37.0 35.0 37.0 32.0 37.0 5 34.81037309032093 37.0 35.0 37.0 32.0 37.0 6 34.768930347137726 37.0 35.0 37.0 32.0 37.0 7 34.618690712063355 37.0 35.0 37.0 31.0 37.0 8 34.536705038320775 37.0 35.0 37.0 31.0 37.0 9 36.09481757352952 39.0 37.0 39.0 31.0 39.0 10 35.93124162028295 39.0 35.0 39.0 30.0 39.0 11 35.93007966515535 39.0 35.0 39.0 30.0 39.0 12 35.77573636120039 39.0 35.0 39.0 30.0 39.0 13 35.756852409893966 39.0 35.0 39.0 30.0 39.0 14 36.84986696631348 40.0 37.0 41.0 31.0 41.0 15 36.79793610022081 40.0 36.0 41.0 30.0 41.0 16 36.70790299079885 40.0 36.0 41.0 30.0 41.0 17 36.65770245905174 40.0 36.0 41.0 30.0 41.0 18 36.59050433601145 40.0 36.0 41.0 30.0 41.0 19 36.612839749525506 40.0 36.0 41.0 30.0 41.0 20 36.51139156967152 40.0 36.0 41.0 30.0 41.0 21 36.40966525710559 40.0 36.0 41.0 30.0 41.0 22 36.306271601923285 40.0 36.0 41.0 30.0 41.0 23 36.37691391889476 40.0 36.0 41.0 30.0 41.0 24 36.28589183932457 40.0 36.0 41.0 30.0 41.0 25 35.885122952587096 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10451986414760128 1101 2 0.036630581957027175 1101 3 0.11202753880050409 1101 4 0.005889812780914383 1101 5 0.13113743095391328 1101 6 0.13590855554968329 1101 7 0.06452411187135709 1101 8 0.006083628683640541 1101 9 0.10263584926276081 1101 10 0.021654861203714404 1101 11 0.14487903054494922 1101 12 -0.007637634319365816 1101 13 0.10305575828446933 1101 14 -0.06461483301950466 1101 15 0.030354579952764027 1101 16 -0.11669656671307393 1101 17 0.06944702079788101 1101 18 -0.08065690789712221 1101 19 0.024658414482999547 1101 20 -0.10061728739722042 1101 21 -0.17101657219927802 1101 22 -0.330364216472006 1101 23 -0.23846199123585166 1101 24 -0.4029497208670705 1101 25 -0.41625265071327533 1102 1 0.07789854404017049 1102 2 0.07721817087346139 1102 3 0.14642065241746494 1102 4 0.1749126607058571 1102 5 -0.09378653863484487 1102 6 0.12371269441430144 1102 7 0.15135467575228034 1102 8 0.12157799051722407 1102 9 0.20821950980734982 1102 10 0.23612926984714022 1102 11 0.30839113732319134 1102 12 0.3607515770995917 1102 13 0.4245742817860503 1102 14 0.420220641441027 1102 15 0.2765156353921725 1102 16 0.20553393113630847 1102 17 0.3517458828787383 1102 18 0.23227246360470843 1102 19 0.13219753633691766 1102 20 0.35662981247250514 1102 21 0.2833103078285646 1102 22 0.4265372968810155 1102 23 0.43358485350782416 1102 24 0.4701769554640123 1102 25 0.4947475710755782 1103 1 0.194957403137213 1103 2 0.16736211308317195 1103 3 0.16402840900681426 1103 4 0.20342989944825973 1103 5 0.30647470562765733 1103 6 0.2524857533552023 1103 7 0.2098683221637785 1103 8 0.17158296998580624 1103 9 0.299893304820408 1103 10 0.2592909122892877 1103 11 0.188137860210432 1103 12 0.20072657305377817 1103 13 0.19448459800008777 1103 14 0.3590798807560631 1103 15 0.2871419102695043 1103 16 0.4985014098713165 1103 17 0.33620104898847103 1103 18 0.2629242049070868 1103 19 0.29003112961036237 1103 20 0.36997745585008346 1103 21 0.6800716970291631 1103 22 0.45529239416737965 1103 23 0.3687583968798833 1103 24 0.6693396933892473 1103 25 0.45298583509055845 1104 1 0.05156081869698781 1104 2 0.09314958936400686 1104 3 0.11219539192901351 1104 4 0.08776386529265778 1104 5 0.07303258039046057 1104 6 0.03953319168537206 1104 7 -0.05897797764647095 1104 8 0.10417879541274999 1104 9 0.15073960463065816 1104 10 0.08162108306727589 1104 11 0.3122744826013246 1104 12 0.4121724933573816 1104 13 0.239031087563653 1104 14 0.3282032204145082 1104 15 0.3564360279165655 1104 16 0.47572721632292314 1104 17 0.3277835916829659 1104 18 0.4263815104099322 1104 19 0.5034087387185266 1104 20 0.35992811998670504 1104 21 0.4801855490215772 1104 22 0.4094973277537193 1104 23 0.40270906101650183 1104 24 0.2903491810552836 1104 25 0.49778279903742373 1105 1 -6.786225340533747E-4 1105 2 -0.07670853570420277 1105 3 -0.061060746890149176 1105 4 0.03814736414674513 1105 5 0.03521810914742218 1105 6 -0.05571465290700672 1105 7 -0.020823661374507196 1105 8 -0.06707319629302333 1105 9 -0.12804179883982414 1105 10 -0.11223922469219616 1105 11 -0.12551663384525824 1105 12 -0.04235746408014052 1105 13 -0.025892409107740377 1105 14 0.06677879609932802 1105 15 0.017224852537324864 1105 16 -0.14091711413652774 1105 17 0.12623759146470093 1105 18 -0.10459611700466809 1105 19 -0.011902840458603237 1105 20 -0.007738146519898237 1105 21 -0.15571863083218318 1105 22 -0.0714209304586575 1105 23 -0.19863307583823797 1105 24 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10 0.08274509535876007 1107 11 0.05629116390070976 1107 12 0.1851869797489485 1107 13 0.0752683570983379 1107 14 0.23004496756112758 1107 15 0.11796939051533428 1107 16 0.12154723388218969 1107 17 0.11802898237655768 1107 18 0.21207049197271033 1107 19 0.14078801878876135 1107 20 0.08335612701041129 1107 21 -0.0618699821461135 1107 22 0.01676839886873438 1107 23 0.14990484679350402 1107 24 -0.05903426933980427 1107 25 0.04379926084602914 1108 1 0.029891997322280872 1108 2 0.047470856332331834 1108 3 -0.017883685855945686 1108 4 0.029489355488735214 1108 5 -0.02421650385862506 1108 6 0.07238343947679482 1108 7 -0.06711070766155558 1108 8 0.009635808987411565 1108 9 -0.09712033934693665 1108 10 -0.11152565255004987 1108 11 -0.15246587508021037 1108 12 -0.01930908388889918 1108 13 0.02001406537016237 1108 14 -0.03247314243832733 1108 15 0.14116044075862533 1108 16 0.0799215709878709 1108 17 0.15465560202557072 1108 18 -0.12467127993697602 1108 19 0.0782094282250938 1108 20 0.1160190486658692 1108 21 0.09817628377701482 1108 22 0.31723208319435514 1108 23 0.08511888648515509 1108 24 -0.02393674870123874 1108 25 0.08807820950930534 1109 1 -0.06626723560165715 1109 2 0.013421082576677179 1109 3 -0.02505347684926562 1109 4 -0.02631758267114037 1109 5 -0.22989954630637044 1109 6 -0.16522698931009927 1109 7 -0.07193725366457215 1109 8 -0.031314417256929516 1109 9 -0.12440971663631473 1109 10 -0.11459611150286264 1109 11 0.012907966764217349 1109 12 -0.11287728039827272 1109 13 -0.11729470429154532 1109 14 -0.11226736782078461 1109 15 -0.11760922095221105 1109 16 -0.10392889227436086 1109 17 -0.053085186597570555 1109 18 -0.08183568416019682 1109 19 -0.09523379521812814 1109 20 -0.2576827397418029 1109 21 -0.02153390799200139 1109 22 -0.016602219757594128 1109 23 -0.07383392540883449 1109 24 -0.07099018546055191 1109 25 -0.21345701996710176 1110 1 -0.3696389273884968 1110 2 -0.3393927297219008 1110 3 -0.3233244980413623 1110 4 -0.41730321045850616 1110 5 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-0.6166017331458633 2314 15 -0.5416764591835417 2314 16 -0.49156483734064693 2314 17 -0.4729470584718314 2314 18 -0.46569692096261406 2314 19 -0.7220556575663082 2314 20 -0.628423789198024 2314 21 -0.5632561434448888 2314 22 -0.5208968930925124 2314 23 -0.3518822702528439 2314 24 -0.2900780635267637 2314 25 -0.2912017210864164 2315 1 -0.4897615707238998 2315 2 -0.5747756272342599 2315 3 -0.6534480189236973 2315 4 -0.48829450224040016 2315 5 -0.6302888970377154 2315 6 -0.6561421389119815 2315 7 -0.7260605129861872 2315 8 -0.9224295037208563 2315 9 -0.9015467610784142 2315 10 -0.7487993455692958 2315 11 -0.6673333605359346 2315 12 -0.8678493944675196 2315 13 -0.9070628476848626 2315 14 -0.9957147002421252 2315 15 -0.9735740040031828 2315 16 -1.2132978634764342 2315 17 -1.184745364085387 2315 18 -1.1102021649206648 2315 19 -1.254365999083575 2315 20 -1.063116111659447 2315 21 -1.2149535298657952 2315 22 -1.0780284360401708 2315 23 -1.0960269170956707 2315 24 -0.9976800027112489 2315 25 -1.103800420296821 2316 1 -0.9743173990224392 2316 2 -1.0895610484939482 2316 3 -1.1444174479345222 2316 4 -1.029971687276344 2316 5 -1.2103979995743899 2316 6 -1.335027544384097 2316 7 -1.32145931745832 2316 8 -1.287951050550241 2316 9 -1.3744029571392176 2316 10 -1.6528648054884982 2316 11 -1.7366586301868523 2316 12 -1.882231311046631 2316 13 -1.81099206967815 2316 14 -2.158804119604099 2316 15 -2.032407350559609 2316 16 -2.1071893555782424 2316 17 -2.087737549638234 2316 18 -1.9373430838214318 2316 19 -1.8259151525251411 2316 20 -1.8219126739758735 2316 21 -1.7849431597554357 2316 22 -1.9535734879187743 2316 23 -2.0833664067804563 2316 24 -2.057648131050577 2316 25 -2.057726556901784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21400.0 3 2.0 4 370.0 5 3449.0 6 4581.0 7 3691.0 8 3045.0 9 2872.0 10 2614.0 11 2582.0 12 2541.0 13 2453.0 14 2371.0 15 2475.0 16 2550.0 17 2671.0 18 2843.0 19 3336.0 20 4079.0 21 5257.0 22 6891.0 23 8857.0 24 10738.0 25 13349.0 26 15486.0 27 17820.0 28 21295.0 29 26150.0 30 34220.0 31 45050.0 32 58043.0 33 77439.0 34 101841.0 35 136396.0 36 198332.0 37 343911.0 38 872763.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.74389093489797 19.88871722531963 13.596533499220701 24.7708583405617 2 14.904763773747277 21.320810577571166 40.26663914412653 23.50778650455503 3 17.846768257789293 25.595768506364347 30.888042861510744 25.669420374335616 4 12.552701061119906 17.91906338082425 38.608696831952116 30.919538726103724 5 13.09168025848042 38.00431123928251 36.004375238786025 12.899633263451046 6 30.772574176395256 38.016429212075224 18.4590963206531 12.751900290876423 7 27.157575748765726 32.288978918606446 23.002738202012537 17.55070713061529 8 24.99119291323893 36.596583092622446 21.11074445201991 17.30147954211871 9 26.264353028908843 15.391689840354731 20.607259651423153 37.73669747931328 10 14.881117647714392 29.214158796334655 34.67045392324603 21.23426963270492 11 33.58646317430829 22.906700042592103 24.95834066217875 18.548496120920863 12 22.579143050825117 25.201973288599515 32.1370234857394 20.081860174835967 13 28.478318489090075 22.079909369438255 26.335194496654896 23.106577644816774 14 21.09413726285431 21.999376866432822 28.117715067088618 28.788770803624253 15 22.888093254894095 30.345199521456678 24.041762547346764 22.724944676302464 16 22.231574071247522 28.302377743956065 27.421171907820813 22.044876276975604 17 21.65016041086113 28.478754585676747 27.970798972556445 21.90028603090568 18 21.477805348773092 27.755657989798248 29.520783151941384 21.24575350948728 19 23.01844883276269 27.25910255155391 28.068352843011933 21.65409577267146 20 23.16787344283234 27.32721732098114 28.42899111961984 21.07591811656668 21 23.028225624744703 26.90589956307968 27.72261155956377 22.343263252611855 22 22.983259221141186 27.47030545658586 27.984463332272163 21.56197199000079 23 22.45572771679694 27.35638733711187 28.025407956243036 22.162476989848155 24 22.607650227679002 27.065380725713794 28.539031066784066 21.787937979823138 25 22.6008887777634 27.27969170430477 28.203842260602507 21.915577257329318 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 742.0 1 742.0 2 723.5 3 705.0 4 705.0 5 705.0 6 1776.5 7 2848.0 8 2848.0 9 2848.0 10 4069.0 11 5290.0 12 5290.0 13 5290.0 14 8555.0 15 11820.0 16 11820.0 17 11820.0 18 20366.5 19 28913.0 20 28913.0 21 28913.0 22 43901.5 23 58890.0 24 58890.0 25 58890.0 26 81986.5 27 105083.0 28 105083.0 29 105083.0 30 131958.5 31 158834.0 32 158834.0 33 158834.0 34 179015.0 35 199196.0 36 199196.0 37 199196.0 38 215281.5 39 231367.0 40 231367.0 41 231367.0 42 243787.5 43 256208.0 44 256208.0 45 256208.0 46 258604.0 47 261000.0 48 261000.0 49 261000.0 50 253455.0 51 245910.0 52 245910.0 53 245910.0 54 219787.0 55 193664.0 56 193664.0 57 193664.0 58 165712.0 59 137760.0 60 137760.0 61 137760.0 62 111928.0 63 86096.0 64 86096.0 65 86096.0 66 66336.0 67 46576.0 68 46576.0 69 46576.0 70 33782.5 71 20989.0 72 20989.0 73 20989.0 74 14536.5 75 8084.0 76 8084.0 77 8084.0 78 5421.5 79 2759.0 80 2759.0 81 2759.0 82 1771.5 83 784.0 84 784.0 85 784.0 86 484.0 87 184.0 88 184.0 89 184.0 90 115.5 91 47.0 92 47.0 93 47.0 94 29.5 95 12.0 96 12.0 97 12.0 98 7.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4533950846100061 2 0.0 3 0.0 4 0.0 5 0.06042360484222268 6 0.0 7 0.0 8 0.004070234808938817 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.845517629689068E-5 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005426979745251756 25 0.0014536552889067205 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2063763.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.24472266438877 #Duplication Level Percentage of deduplicated Percentage of total 1 82.84299744032482 59.84969374762936 2 11.639706570247055 16.818147461247246 3 2.924902290952041 6.339262644907967 4 1.048870806340867 3.0310152205947904 5 0.5081052666636833 1.8353962037216547 6 0.2697730204865774 1.1693806228432353 7 0.17037367618650082 0.8616019289784291 8 0.11637081275618301 0.6725741675039966 9 0.0859154166218366 0.5586241901796003 >10 0.36483423947561006 4.385982991194207 >50 0.015472377863822061 0.7836228221993355 >100 0.01033201455416508 1.4759639853513071 >500 0.0015417015176902701 0.7651307902231979 >1k 7.373355084604005E-4 1.1476961853346064 >5k 6.703050076912732E-5 0.3059070380910847 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6317 0.3060913486674584 No Hit TATCAACGCAGAGTACTTTTTTTTT 4572 0.22153706602938417 No Hit GGTATCAACGCAGAGTACTTTTTTT 4318 0.20922945124997394 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 4.8455176296890677E-5 10 0.0 0.0 0.0 0.0 4.8455176296890677E-5 11 0.0 0.0 0.0 0.0 4.8455176296890677E-5 12 0.0 0.0 0.0 0.0 4.8455176296890677E-5 13 0.0 0.0 0.0 0.0 4.8455176296890677E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1335 0.0 12.151493 1 TACGATG 55 0.0030647232 12.093178 5 TAGGACC 455 0.0 11.6945 4 GGCGAGG 660 0.0 10.936144 19 GTTCTAA 230 0.0 10.787168 1 CAATGCG 75 0.002656871 10.129341 9 TGGCGAG 1305 0.0 9.897202 18 TTAGGAC 980 0.0 9.889609 3 GTCCTAG 290 0.0 9.871548 1 TAAGACA 300 0.0 9.818508 4 GTATCAA 2505 0.0 9.713918 1 GACGTGA 785 0.0 9.678184 7 GTCCTAA 840 0.0 9.656096 1 GCACCGT 80 0.0045294045 9.496717 6 ACAGCGC 90 0.0011173654 9.496717 8 ATTATAC 255 0.0 9.315473 3 TATGGCG 1410 0.0 9.294209 16 AGGACGT 1330 0.0 9.216013 5 ATGGCGA 1435 0.0 9.198465 17 CGTGAAA 870 0.0 9.168799 9 >>END_MODULE