FastQCFastQC Report
Thu 26 May 2016
SRR1512097_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512097_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2350563
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46980.1998670105842728No Hit
TATCAACGCAGAGTACTTTTTTTTT29320.1247360738682605No Hit
GGTATCAACGCAGAGTACTTTTTTT28950.12316198289516173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA550.00318151412.0330397
TACACGG1104.080721E-812.0330395
GGTATCA10950.011.7593691
TAGGACC4650.011.1827784
GTATCAA22400.010.4798521
GTCCTAC11800.010.1568291
CTACACG856.8929576E-410.010684
TATGTCG1054.3288626E-59.90474616
GGACGTG11450.09.7435346
ATAGGAC2350.09.6556913
AGGACGT11750.09.4947625
TCCTACA13500.09.4649962
TACATCG800.00469919539.4545315
GCCGTCC800.00469919539.4545318
TAGGACG12450.09.3406214
TATACTG4050.09.3378095
GTCAACG850.00527603979.3225682
TGTAGGA12700.09.2812772
TCAGGAC4600.09.2489983
GTATAAG2601.8189894E-129.1430841
Produced by FastQC (version 0.11.5)