FastQCFastQC Report
Thu 26 May 2016
SRR1512097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2350563
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58250.24781297076487632No Hit
TATCAACGCAGAGTACTTTTTTTTT36190.15396311436877036No Hit
GGTATCAACGCAGAGTACTTTTTTT35990.15311225438331158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCTC707.290406E-613.5670828
GGTATCA12600.012.8714041
TAGACCG658.014726E-411.6942845
GGCGAGG6400.010.98085719
TAGGACC5300.010.39798454
TTAGGAC9000.010.3461943
TGGCGAG12800.010.23890818
GTATCAA26550.010.1687941
ACTGTTC5900.010.140828
GTCCTAA5500.09.886891
ATGGCGA13550.09.81193717
CGTGAAA7650.09.8068999
AGGACGT12350.09.7708845
GTAGGAC11450.09.7090653
TAGGACG12650.09.6893854
GGACGTG12850.09.6817246
ATAGAAC2750.09.6743643
TAAGACT2750.09.6743644
TATGGCG13750.09.60015316
CCGGATC900.00111766239.4965549
Produced by FastQC (version 0.11.5)