FastQCFastQC Report
Thu 26 May 2016
SRR1512096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959043
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65370.3336833341585662No Hit
TATCAACGCAGAGTACTTTTTTTTT41470.21168499109003736No Hit
GGTATCAACGCAGAGTACTTTTTTT41360.21112349243993114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1600.013.59193219
TCCAACG1950.013.09189418
CGTTAGG600.00432726711.5478081
ATCCCGT1604.9112714E-1111.22811810
TCCCGTT1604.9112714E-1111.22811811
GGTATCA17300.010.9279841
GGCGAGG5800.010.75943119
CCTTGCG909.983494E-510.5058429
TAGGACC3650.010.3619264
GTATAAG1853.074092E-1010.1656381
TGGCGAG10050.010.06679218
CCCGTTT1701.6916601E-910.01144912
CCGTTTC1701.6916601E-910.01144913
TTAGGAC7850.09.9972783
CGTGAAA7300.09.9733539
GACGTGA6650.09.9529037
GTATTAC1501.4569741E-79.8981221
GTCCTAT1603.5182893E-89.8981221
TACTAGG1104.359497E-59.8981212
GTCCTAC10750.09.8520841
Produced by FastQC (version 0.11.5)