FastQCFastQC Report
Thu 26 May 2016
SRR1512096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959043
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80910.4130077798190239No Hit
TATCAACGCAGAGTACTTTTTTTTT55140.2814639596986896No Hit
GGTATCAACGCAGAGTACTTTTTTT52130.26609931481851085No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26420.13486176668914363No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21340.10893073812060275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC655.4727734E-513.14900410
TAGGACC3900.012.9130854
TCCAACG1052.003435E-812.6626518
GGTATCA19900.011.4556391
GCGTGCG752.0814451E-411.3958039
TGCGCCA951.3661471E-510.99595312
ATAATAC2250.010.5578683
CCAACGA909.5476986E-510.55220919
TTATACT3000.010.452294
CTAATAC1303.8305552E-710.233013
GGCGAGG4550.010.22752619
TATGTCG750.00265619710.12960316
GTAGGAC11100.010.0157073
GGCGTGC951.6495878E-49.996838
CTAGGAC2400.09.8980013
CCCCTAT1356.0977254E-79.891651
GTATTAG1451.5249498E-79.8672861
GTATAAT2750.09.71180251
GTACATA5750.09.621321
GTCCTAT2303.6379788E-129.5383771
Produced by FastQC (version 0.11.5)