FastQCFastQC Report
Thu 26 May 2016
SRR1512095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086550
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41600.19937216937049193No Hit
GTCCTACAGTGGACATTTCTAAATT26790.12839376003450673No Hit
GTCCTAAAGTGTGTATTTCTCATTT26280.1259495339196281No Hit
TATCAACGCAGAGTACTTTTTTTTT24510.11746663152093167No Hit
CTGTAGGACGTGGAATATGGCAAGA24410.11698737149840646No Hit
GGTATCAACGCAGAGTACTTTTTTT24310.11650811147588123No Hit
CTTTAGGACGTGAAATATGGCGAGG23570.1129615873091946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1750.012.96580218
GGTATCA12150.011.8240041
ACTCGCC600.00609184611.0299368
TAGGACC6250.010.7400064
CCCTTAC1501.2723831E-810.5682121
CTATACT1909.094947E-1210.4494124
CTAGTAC1201.6215563E-610.2420843
GGCGAGG8000.010.16329819
GTCCTAA8600.010.138111
GGACGTG17150.09.9228096
GACGTGG8500.09.8991377
CTAGCAC1252.7341866E-69.83243
AGGACGT17350.09.8084245
CCAACGA1554.3795808E-89.75920619
ACGTGGA9100.09.6620168
GTAGGAC17300.09.6181763
CGTGGAA9050.09.610939
GGCTTAC1251.676649E-59.5113931
GTATCAA21900.09.5005341
TGTAGGA16700.09.4924072
Produced by FastQC (version 0.11.5)