##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512095_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2086550 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.632759818839713 31.0 28.0 33.0 16.0 34.0 2 26.070395629148596 31.0 25.0 34.0 10.0 34.0 3 27.74689751024418 31.0 26.0 34.0 16.0 34.0 4 32.002680980566005 35.0 32.0 37.0 19.0 37.0 5 32.39846445088783 35.0 33.0 37.0 22.0 37.0 6 32.64444705374901 35.0 33.0 37.0 25.0 37.0 7 32.674384510316074 36.0 33.0 37.0 25.0 37.0 8 32.70416764515588 37.0 33.0 37.0 25.0 37.0 9 34.210280127483166 39.0 35.0 39.0 25.0 39.0 10 34.05616639907982 38.0 34.0 39.0 25.0 39.0 11 34.0624787328365 38.0 35.0 39.0 25.0 39.0 12 33.951204140806595 38.0 34.0 39.0 25.0 39.0 13 33.9393659389902 38.0 34.0 39.0 25.0 39.0 14 34.975079916608756 39.0 35.0 41.0 24.0 41.0 15 34.90448108121061 39.0 35.0 41.0 24.0 41.0 16 34.87082360834871 39.0 34.0 41.0 23.0 41.0 17 34.80394862332559 39.0 34.0 41.0 22.0 41.0 18 34.74308978936522 39.0 34.0 41.0 22.0 41.0 19 34.75011957537562 39.0 34.0 41.0 21.0 41.0 20 34.66980374302078 39.0 34.0 41.0 19.0 41.0 21 34.59207783182766 39.0 34.0 41.0 18.0 41.0 22 34.47492319858139 39.0 34.0 41.0 18.0 41.0 23 34.35336704128825 39.0 34.0 41.0 18.0 41.0 24 34.231767750593086 39.0 34.0 41.0 16.0 41.0 25 34.099241810644365 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1557745781342028 1101 2 3.4540341894600175 1101 3 1.9483508397854266 1101 4 1.271669435502126 1101 5 0.9387206265995545 1101 6 0.6395177076314909 1101 7 0.5657311193288521 1101 8 0.5517528636249196 1101 9 0.5618204775061031 1101 10 0.4595756236904478 1101 11 0.6509720848235148 1101 12 0.3729523811559545 1101 13 0.46450363364454006 1101 14 0.45208113120113325 1101 15 0.525000528876248 1101 16 0.5436632606812637 1101 17 0.2868825863738138 1101 18 0.27926136425419656 1101 19 0.44144274798257044 1101 20 0.4252931639037314 1101 21 0.48801569401264544 1101 22 0.3957590126639303 1101 23 0.45447384341550645 1101 24 0.4144620223048534 1101 25 0.24469665517876393 1102 1 1.046445829176971 1102 2 3.5307796374128984 1102 3 2.1510132263487414 1102 4 1.133470273773554 1102 5 0.8422884781550195 1102 6 0.5628675984736446 1102 7 0.4897466068118348 1102 8 0.4405094783877246 1102 9 0.4497400886455267 1102 10 0.5286138428834661 1102 11 0.4796807259160545 1102 12 0.43634887644099507 1102 13 0.4178469572665051 1102 14 0.3219586360631581 1102 15 0.3571385774229654 1102 16 0.5810355612114577 1102 17 0.3911010744932355 1102 18 0.17431794630768138 1102 19 0.275999764892525 1102 20 0.4353831018574468 1102 21 0.3557731784883984 1102 22 0.43819517243755257 1102 23 0.1520718636141467 1102 24 0.21405810022910288 1102 25 0.3662234451266073 1103 1 0.7960325503347647 1103 2 2.5318800804086017 1103 3 1.550361293527235 1103 4 0.9940399797297914 1103 5 0.5719312018127098 1103 6 0.4753823803151107 1103 7 0.3656894278918088 1103 8 0.2448732618038747 1103 9 0.4426153942390343 1103 10 0.32773520409958934 1103 11 0.2578532307718504 1103 12 0.40609527212254903 1103 13 0.38118921587060584 1103 14 0.2984571931103446 1103 15 0.07518621018381566 1103 16 -0.03176658575527824 1103 17 0.1785023501738081 1103 18 0.18319991828192883 1103 19 0.08147502024309716 1103 20 0.31438338983529235 1103 21 0.469034251849223 1103 22 0.24617399687522834 1103 23 0.5193220428344034 1103 24 0.2732013967788802 1103 25 0.3969169815558047 1104 1 1.2640174937278523 1104 2 3.7664326549895506 1104 3 2.4299957019479415 1104 4 1.566384131433665 1104 5 1.2743209500134967 1104 6 0.8723869688919947 1104 7 0.7840430122730524 1104 8 0.8438367038512453 1104 9 0.976269658771038 1104 10 0.9743347516299963 1104 11 0.8665017399966146 1104 12 0.9689151436616825 1104 13 1.0011393405588862 1104 14 1.10333600622738 1104 15 0.8259752597927559 1104 16 1.0269631004207795 1104 17 0.9784517275761218 1104 18 1.0357085693222814 1104 19 1.0206228008640394 1104 20 0.9201027791339129 1104 21 0.8801326537420238 1104 22 0.9766853212708639 1104 23 1.010144286403829 1104 24 1.149940192611922 1104 25 1.1367667288646714 1105 1 1.0498013387136034 1105 2 3.43113529646525 1105 3 1.9726706375605367 1105 4 1.4170512106367141 1105 5 1.0222189945493625 1105 6 0.6293938537876329 1105 7 0.4165027396053418 1105 8 0.40985993264474274 1105 9 0.5102866569065156 1105 10 0.5552806194304551 1105 11 0.3979323573444944 1105 12 0.22354326689364967 1105 13 0.16549008325174697 1105 14 0.1878628117900476 1105 15 0.34861523943149564 1105 16 0.31818221258906476 1105 17 0.3549653613091266 1105 18 0.24203820827806766 1105 19 0.656372706549611 1105 20 0.6211270720767388 1105 21 0.45296852254526243 1105 22 0.663273079547082 1105 23 0.5344148809356071 1105 24 0.6893770572921909 1105 25 0.7276655772443519 1106 1 0.6826248397202797 1106 2 3.0383784944668655 1106 3 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1107 15 0.42265618440399066 1107 16 0.49071075747202286 1107 17 0.35450418423780405 1107 18 0.3345523065420437 1107 19 0.5294573159110527 1107 20 0.5393016289467099 1107 21 0.41426942844805836 1107 22 0.5696404246145121 1107 23 0.5845038350705067 1107 24 0.6885502198431723 1107 25 0.5493936697602493 1108 1 0.7185878104349754 1108 2 2.251984745082094 1108 3 1.4985435542658117 1108 4 1.0356711687921276 1108 5 0.7885145133313287 1108 6 0.6956158665810293 1108 7 0.5456343629758322 1108 8 0.6632279276255133 1108 9 0.5739734631551485 1108 10 0.42370820897230743 1108 11 0.5605844322341298 1108 12 0.5708211186480412 1108 13 0.4937728580542853 1108 14 0.5315886829860261 1108 15 0.5476402041124402 1108 16 0.5050619230831259 1108 17 0.6418635144821891 1108 18 0.8303378285706629 1108 19 0.7250794070660902 1108 20 0.7343518848041342 1108 21 0.6654398768291259 1108 22 0.8104686010645707 1108 23 0.707241975966582 1108 24 0.6822168415922647 1108 25 0.5823674897236941 1109 1 0.8262384392780291 1109 2 3.128012681404993 1109 3 1.5065494998108093 1109 4 1.1775570475876407 1109 5 0.5409235622170172 1109 6 0.3120314817457057 1109 7 0.31532249765370324 1109 8 0.17599784194939616 1109 9 0.2551874509100571 1109 10 0.15823472641584146 1109 11 0.190101733341983 1109 12 0.2763878018621284 1109 13 0.27248489263756426 1109 14 0.20293493483114133 1109 15 0.0901668631557655 1109 16 0.21883913061985538 1109 17 0.24487263367382184 1109 18 0.17668290727792169 1109 19 0.04173463049328774 1109 20 0.19473481346664556 1109 21 0.3429901756293958 1109 22 0.38399306119476506 1109 23 0.34754206051483294 1109 24 0.39826870169495976 1109 25 0.28290964406582475 1110 1 0.18737250054596544 1110 2 1.9246806029530639 1110 3 0.8842053477455458 1110 4 0.31508558773926154 1110 5 0.43782154966380915 1110 6 0.2547226246738532 1110 7 0.2535813632745132 1110 8 0.2445267732983396 1110 9 0.14221245535900806 1110 10 0.12107427619385902 1110 11 0.050049020568827984 1110 12 -0.02875018145945063 1110 13 0.10521566319548725 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-2.4312420125019045 2315 23 -2.1980350398619137 2315 24 -2.1672786350555455 2315 25 -2.295917488696837 2316 1 -1.2324318391502374 2316 2 -1.743607614726649 2316 3 -1.053275257297539 2316 4 -1.4520668480021008 2316 5 -1.4520598526612005 2316 6 -1.6106929512336947 2316 7 -1.5379059414829186 2316 8 -1.735846193380791 2316 9 -1.8256983397745543 2316 10 -1.973520067507323 2316 11 -2.1595272476955856 2316 12 -2.0179251431999994 2316 13 -2.1586465921628246 2316 14 -2.49329431496961 2316 15 -2.549427141757427 2316 16 -2.5404949559612575 2316 17 -2.4601563547458483 2316 18 -2.1912357433285763 2316 19 -2.4360307184682313 2316 20 -2.3908762058238793 2316 21 -2.4151415853162774 2316 22 -2.316998156838345 2316 23 -2.3191637381260435 2316 24 -2.397095338447272 2316 25 -2.643078976561135 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100760.0 3 3.0 4 841.0 5 4167.0 6 4822.0 7 4360.0 8 4098.0 9 4005.0 10 3881.0 11 3680.0 12 3690.0 13 3700.0 14 3780.0 15 3935.0 16 4158.0 17 4344.0 18 4964.0 19 6184.0 20 8009.0 21 10616.0 22 12776.0 23 14855.0 24 17780.0 25 20099.0 26 22655.0 27 25421.0 28 29080.0 29 35123.0 30 43232.0 31 54098.0 32 68233.0 33 88687.0 34 116493.0 35 156355.0 36 225610.0 37 309076.0 38 662980.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.06434065802401 18.780906280702595 12.740360882796962 25.41439217847643 2 16.22816490251368 21.70822312609182 39.10718824116777 22.956423730226735 3 18.530444992930914 24.87105509093959 29.93352663487575 26.664973281253744 4 12.550430135870217 17.248376506673697 38.23833600920179 31.962857348254296 5 13.429632647192735 37.681723419041 35.594641872948166 13.294002060818096 6 31.274975437923846 37.047374853226614 18.601758884282667 13.07589082456687 7 27.694232105628906 31.769619707172126 22.62380484531883 17.91234334188014 8 25.03382385714183 36.435686745313426 21.082315502421466 17.44817389512328 9 26.543912199563874 15.49246363614579 20.405597757063095 37.55802640722724 10 15.426517457046321 28.65557978481225 34.27461599290695 21.64328676523448 11 33.87433802209389 22.522776832570514 24.992355802640724 18.61052934269488 12 23.427955237113895 24.53111595696245 31.7311830533656 20.30974575255805 13 28.810380772087896 21.96127579017996 25.918909204188733 23.309434233543406 14 21.32769336354609 22.04251448333506 27.587398444119827 29.042393708999032 15 23.01210131556876 30.458076729529605 23.583810596439097 22.946011358462535 16 22.415997699551895 27.926433586542377 27.51297596510987 22.144592748795862 17 22.036567539718675 28.20426062160025 27.700702115933 22.05846972274808 18 21.76132850878244 27.437444585559895 29.426325753037307 21.374901152620353 19 23.611641626093256 26.84914358507141 27.380883344587975 22.158331444247363 20 23.605219532557857 27.21865359371248 27.919955792991964 21.256171080737698 21 23.25044846778101 26.633786218296336 27.10758290363658 23.008182410286075 22 23.401538816930245 27.591397808235456 27.25188805571717 21.75517531911712 23 22.751471094279434 27.400071505869523 27.48499670747102 22.363460692380023 24 23.176207558427926 26.751783450632505 28.048376622598603 22.023632368340966 25 23.048285447269414 27.05370108552395 27.763101770865784 22.13491169634085 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 912.0 1 912.0 2 813.0 3 714.0 4 714.0 5 714.0 6 1416.0 7 2118.0 8 2118.0 9 2118.0 10 3076.5 11 4035.0 12 4035.0 13 4035.0 14 6606.5 15 9178.0 16 9178.0 17 9178.0 18 16431.0 19 23684.0 20 23684.0 21 23684.0 22 37511.0 23 51338.0 24 51338.0 25 51338.0 26 74133.5 27 96929.0 28 96929.0 29 96929.0 30 125661.5 31 154394.0 32 154394.0 33 154394.0 34 175037.5 35 195681.0 36 195681.0 37 195681.0 38 212466.5 39 229252.0 40 229252.0 41 229252.0 42 243709.0 43 258166.0 44 258166.0 45 258166.0 46 264121.0 47 270076.0 48 270076.0 49 270076.0 50 263305.0 51 256534.0 52 256534.0 53 256534.0 54 230117.5 55 203701.0 56 203701.0 57 203701.0 58 175333.0 59 146965.0 60 146965.0 61 146965.0 62 120277.5 63 93590.0 64 93590.0 65 93590.0 66 72465.5 67 51341.0 68 51341.0 69 51341.0 70 37581.0 71 23821.0 72 23821.0 73 23821.0 74 16578.0 75 9335.0 76 9335.0 77 9335.0 78 6318.0 79 3301.0 80 3301.0 81 3301.0 82 2189.5 83 1078.0 84 1078.0 85 1078.0 86 695.5 87 313.0 88 313.0 89 313.0 90 193.0 91 73.0 92 73.0 93 73.0 94 43.5 95 14.0 96 14.0 97 14.0 98 10.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.581917519350124 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.3963001126261054E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 6.709640315353095E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 1.437780067575663E-4 20 1.437780067575663E-4 21 4.7926002252522104E-5 22 5.751120270302652E-4 23 3.8340801802017683E-4 24 2.3963001126261054E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2086550.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.24259959203148 #Duplication Level Percentage of deduplicated Percentage of total 1 83.62894293306003 61.25201181550967 2 11.089149609619044 16.243962893469195 3 2.8295937487294793 6.217404058389258 4 1.0293333695100588 3.015642073189673 5 0.47058544303565486 1.7233450589049606 6 0.2665778671776585 1.1714913591474596 7 0.154178950001342 0.7904726970327693 8 0.10682450003354323 0.6259283266060608 9 0.07779942743698719 0.5128409081030905 >10 0.3181293592639024 3.9151339897812205 >50 0.015117742379519591 0.7787531667901976 >100 0.011121388764798111 1.6242974194598951 >500 0.0018519626915957533 0.9212067702530441 >1k 7.936982963979259E-4 1.2075094633635783 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4160 0.19937216937049193 No Hit GTCCTACAGTGGACATTTCTAAATT 2679 0.12839376003450673 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2628 0.1259495339196281 No Hit TATCAACGCAGAGTACTTTTTTTTT 2451 0.11746663152093167 No Hit CTGTAGGACGTGGAATATGGCAAGA 2441 0.11698737149840646 No Hit GGTATCAACGCAGAGTACTTTTTTT 2431 0.11650811147588123 No Hit CTTTAGGACGTGAAATATGGCGAGG 2357 0.1129615873091946 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.7926002252522104E-5 0.0 5 0.0 0.0 0.0 4.7926002252522104E-5 0.0 6 0.0 0.0 0.0 4.7926002252522104E-5 0.0 7 0.0 0.0 0.0 4.7926002252522104E-5 0.0 8 0.0 0.0 0.0 9.585200450504421E-5 0.0 9 0.0 0.0 0.0 9.585200450504421E-5 0.0 10 0.0 0.0 0.0 9.585200450504421E-5 0.0 11 0.0 0.0 0.0 9.585200450504421E-5 0.0 12 0.0 0.0 0.0 9.585200450504421E-5 0.0 13 0.0 0.0 0.0 9.585200450504421E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 175 0.0 12.965802 18 GGTATCA 1215 0.0 11.824004 1 ACTCGCC 60 0.006091846 11.029936 8 TAGGACC 625 0.0 10.740006 4 CCCTTAC 150 1.2723831E-8 10.568212 1 CTATACT 190 9.094947E-12 10.449412 4 CTAGTAC 120 1.6215563E-6 10.242084 3 GGCGAGG 800 0.0 10.163298 19 GTCCTAA 860 0.0 10.13811 1 GGACGTG 1715 0.0 9.922809 6 GACGTGG 850 0.0 9.899137 7 CTAGCAC 125 2.7341866E-6 9.8324 3 AGGACGT 1735 0.0 9.808424 5 CCAACGA 155 4.3795808E-8 9.759206 19 ACGTGGA 910 0.0 9.662016 8 GTAGGAC 1730 0.0 9.618176 3 CGTGGAA 905 0.0 9.61093 9 GGCTTAC 125 1.676649E-5 9.511393 1 GTATCAA 2190 0.0 9.500534 1 TGTAGGA 1670 0.0 9.492407 2 >>END_MODULE