FastQCFastQC Report
Thu 26 May 2016
SRR1512095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086550
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49180.2357000790779037No Hit
GGTATCAACGCAGAGTACTTTTTTT32440.15547195130718172No Hit
TATCAACGCAGAGTACTTTTTTTTT31980.1532673552035657No Hit
GTCCTACAGTGGACATTTCTAAATT29830.14296326471927345No Hit
GTCCTAAAGTGTGTATTTCTCATTT29210.13999185257961708No Hit
CTTTAGGACGTGAAATATGGCGAGG27600.13227576621696102No Hit
CTGTAGGACGTGGAATATGGCAAGA27110.12992739210658744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTT604.104279E-412.6625617
CAAGTCG1501.4551915E-1112.0294326
TAGGACC6200.011.6472484
GGTATCA13100.011.3577581
TCCAACG1950.011.20310718
ACCGTCT951.3655999E-510.9964348
GGACCGT803.783158E-410.6840356
GGCGAGG8250.010.59228219
ACACCGT1002.4080524E-510.4466126
TGGCGAG18100.010.23296118
AGTGCGG750.002656409310.12956210
AGGACGT17750.010.1173065
ACGAAAT951.6506211E-49.9962812
CGAAATC1054.1228617E-59.94867813
TCGAACT1151.0285892E-59.91149319
GTCTTAG2800.09.8782331
TTAGGAC11350.09.7947053
GGACGTG17650.09.7928596
AGGACCT11850.09.7823435
GTATCAA25900.09.7585531
Produced by FastQC (version 0.11.5)