FastQCFastQC Report
Thu 26 May 2016
SRR1512094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986383
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55450.18567611722943775No Hit
GGTATCAACGCAGAGTACTTTTTTT34050.11401752554846448No Hit
TATCAACGCAGAGTACTTTTTTTTT32880.11009974273226174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG550.00317934112.0343135
TCCAACG2100.011.25677218
GGTATCA16100.011.1859121
TAGGACC4900.010.9992914
GTATTAT3950.010.5215111
GTATTAA3200.09.5860041
GTATCAA29550.09.5436241
ACACCGT1201.7986358E-59.4555316
GTCCTAA7650.09.4425081
TTAGGAC8750.09.2934363
TAGCACC2151.1641532E-109.2356354
GTCCTAC11300.09.1946841
GTTCTAG2800.09.1884291
AGGACGT11800.09.1350055
GTAGGAC11850.09.096463
ACCGCAC1252.9123134E-59.077318
TAGGACA5950.09.058244
GTCCTAG4050.09.0400881
GTCCAGG6200.08.9376271
CGGTAGG1000.002007818.9057081
Produced by FastQC (version 0.11.5)