##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512094_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2986383 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.37630739258829 31.0 28.0 33.0 16.0 34.0 2 25.867603385098295 31.0 25.0 33.0 10.0 34.0 3 27.509973770946324 31.0 26.0 34.0 16.0 34.0 4 31.7298363940593 35.0 32.0 37.0 19.0 37.0 5 32.112688493070046 35.0 33.0 37.0 19.0 37.0 6 32.35377645800957 35.0 33.0 37.0 22.0 37.0 7 32.37859075677835 35.0 33.0 37.0 22.0 37.0 8 32.41134877877352 36.0 33.0 37.0 23.0 37.0 9 33.898159077385586 38.0 34.0 39.0 23.0 39.0 10 33.73849201525725 38.0 34.0 39.0 23.0 39.0 11 33.74908141387089 38.0 34.0 39.0 23.0 39.0 12 33.62852554411139 38.0 34.0 39.0 22.0 39.0 13 33.61096718003016 38.0 34.0 39.0 22.0 39.0 14 34.6195675504448 39.0 34.0 41.0 19.0 41.0 15 34.53937488928915 39.0 34.0 41.0 19.0 41.0 16 34.496012065431664 39.0 34.0 41.0 18.0 41.0 17 34.41691370463869 39.0 34.0 41.0 18.0 41.0 18 34.357885442021335 39.0 34.0 41.0 17.0 41.0 19 34.368417245879044 39.0 34.0 41.0 18.0 41.0 20 34.2751144109781 39.0 34.0 41.0 18.0 41.0 21 34.19765850528884 39.0 34.0 41.0 17.0 41.0 22 34.07439300317474 39.0 34.0 41.0 11.0 41.0 23 33.95060747399111 39.0 34.0 41.0 10.0 41.0 24 33.81574399532813 39.0 34.0 41.0 2.0 41.0 25 33.66896007645369 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9949640372351105 1101 2 3.3837301624214327 1101 3 1.9130431448954042 1101 4 1.1526848718338663 1101 5 0.9513993297349259 1101 6 0.5211652590877094 1101 7 0.48835176016492454 1101 8 0.490601296944547 1101 9 0.35877298459913476 1101 10 0.17393035314039906 1101 11 0.38881819645677496 1101 12 0.05561062043359044 1101 13 0.14330747237123376 1101 14 0.2050632642428809 1101 15 0.2779562164547471 1101 16 0.31280769819280607 1101 17 0.09293490952135386 1101 18 0.17250578160589214 1101 19 0.2397186384082346 1101 20 0.299958788033031 1101 21 0.3300073599417601 1101 22 0.30172500456153983 1101 23 0.16906441899723745 1101 24 0.10828331665211977 1101 25 -0.14685319359476523 1102 1 1.294905386354941 1102 2 3.7974132070814903 1102 3 2.504678694184893 1102 4 1.5845398414831244 1102 5 1.3094308541155542 1102 6 1.1604744377323115 1102 7 0.9975547127786228 1102 8 0.9868440879037124 1102 9 0.9154069850316944 1102 10 0.9548071744496838 1102 11 1.0720077073607897 1102 12 1.0553403651557929 1102 13 0.9505765664737993 1102 14 1.0409787945097833 1102 15 1.1219865104757858 1102 16 1.121522184336925 1102 17 0.9236710871418978 1102 18 0.9503928415762601 1102 19 0.9374438443875093 1102 20 1.053868420801237 1102 21 1.0331694588828668 1102 22 1.1578540838673774 1102 23 1.0854353692973149 1102 24 0.907271978520761 1102 25 0.8333899987583067 1103 1 0.9701376525990746 1103 2 2.800331778832934 1103 3 1.8850326018295256 1103 4 1.1241208859953353 1103 5 0.8708631152834911 1103 6 0.6845228018068497 1103 7 0.6835294212296503 1103 8 0.5272166566166945 1103 9 0.8834959538505487 1103 10 0.638225654950439 1103 11 0.8066451399652053 1103 12 0.6685249417344536 1103 13 0.6647465296471964 1103 14 0.6813433863412186 1103 15 0.6455398244470487 1103 16 0.4464982839077436 1103 17 0.6609115212292878 1103 18 0.7058065456170368 1103 19 0.8314293965629602 1103 20 0.80463849202512 1103 21 1.0051803944622932 1103 22 0.7964284701007287 1103 23 0.9813422621690009 1103 24 0.9318862177377696 1103 25 0.9532335793628803 1104 1 1.3117273059608685 1104 2 3.818626968702027 1104 3 2.3063057449883395 1104 4 1.762887973643803 1104 5 1.261882444491512 1104 6 1.021839890262406 1104 7 0.9289572854077051 1104 8 0.737363042822949 1104 9 0.8972425083102564 1104 10 1.0318164989998024 1104 11 0.9545531759668862 1104 12 0.8623508848606107 1104 13 0.7508976178349869 1104 14 0.9926493161362302 1104 15 0.7988199598094283 1104 16 0.9273233715239328 1104 17 0.9595932192978864 1104 18 0.9515522588094569 1104 19 0.9572413476859012 1104 20 0.7069805099082203 1104 21 0.7677913330260466 1104 22 0.7940855501815918 1104 23 0.9731503506786794 1104 24 1.0702279241810615 1104 25 0.9272219459465276 1105 1 1.3757258011881461 1105 2 3.727378664767155 1105 3 2.244439315481646 1105 4 1.6846776853081629 1105 5 1.2244471141080666 1105 6 0.8814901584370105 1105 7 0.6545905591466834 1105 8 0.7135943906719788 1105 9 0.6478515395032503 1105 10 0.7268037248672812 1105 11 0.6117959904032091 1105 12 0.50886991092203 1105 13 0.5309980942854793 1105 14 0.6828209040549496 1105 15 0.7028194776977799 1105 16 0.7555825101876366 1105 17 0.8189590598613776 1105 18 0.6098825637268064 1105 19 0.7856684949545709 1105 20 0.7451495083030082 1105 21 0.7186250757281698 1105 22 0.8947633370699748 1105 23 0.851825767184792 1105 24 0.8460359633928078 1105 25 0.875553821379107 1106 1 0.5662522198829727 1106 2 2.9777382476510112 1106 3 1.53505729956224 1106 4 0.7573240749846981 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0.7854055635467674 1107 17 0.7426386632429356 1107 18 0.5610513719087322 1107 19 0.8605426677899288 1107 20 0.9977024152960539 1107 21 0.8079505334726775 1107 22 0.9784738890271356 1107 23 0.8788102613525837 1107 24 1.0411885507357823 1107 25 1.2151837703472523 1108 1 0.6357346971324382 1108 2 2.343317252927964 1108 3 1.5199025713546526 1108 4 0.9382065589955495 1108 5 0.7945879921224304 1108 6 0.8119739776601733 1108 7 0.5965712573334514 1108 8 0.7282471356970888 1108 9 0.6470040176074221 1108 10 0.5327916156465591 1108 11 0.6768111556875525 1108 12 0.7922985092526815 1108 13 0.6822415027443753 1108 14 0.5671992978172185 1108 15 0.4810356619256311 1108 16 0.6739689316023245 1108 17 0.7264161022070041 1108 18 0.7460371639908772 1108 19 0.731735341499963 1108 20 0.7709442318321464 1108 21 0.6193033275728652 1108 22 0.7100298724277323 1108 23 0.8084532456830686 1108 24 0.8859794136473056 1108 25 0.7848421373146479 1109 1 0.7107498565975838 1109 2 3.073981967976877 1109 3 1.4967688378923292 1109 4 1.0015384491339923 1109 5 0.5683475128436228 1109 6 0.2362217826080908 1109 7 0.2388529612819994 1109 8 0.0703438088418551 1109 9 0.17129492110957045 1109 10 0.14211865968846382 1109 11 0.1832198584191005 1109 12 0.10154321325518367 1109 13 0.2018909020724564 1109 14 0.027883285387396484 1109 15 0.009077491099688473 1109 16 0.10927241832369816 1109 17 0.14053234361454514 1109 18 0.08623272627016121 1109 19 -0.07078775381077662 1109 20 0.21738596725279535 1109 21 0.3647598531270333 1109 22 0.2899040017634462 1109 23 0.20830267202049413 1109 24 0.33277193519485593 1109 25 0.3783937689189827 1110 1 -0.004653736231965411 1110 2 1.8042424971762756 1110 3 0.7241998904599392 1110 4 0.258916276090563 1110 5 0.2556077350275743 1110 6 0.11221288664831519 1110 7 0.10039216277687046 1110 8 0.02337082946527147 1110 9 -0.046285524521714194 1110 10 -0.21005033052256294 1110 11 -0.27263615882445436 1110 12 -0.14651317160048904 1110 13 -0.15528604961168213 1110 14 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-2.205643872911658 2315 22 -2.410541824508428 2315 23 -2.2272644992961084 2315 24 -2.197973857065115 2315 25 -2.3353166082185908 2316 1 -1.5604931470375618 2316 2 -1.9428104342317312 2316 3 -1.407935014003634 2316 4 -1.6247389388158453 2316 5 -1.6324336431861894 2316 6 -1.740736272306286 2316 7 -1.7421449343057382 2316 8 -1.9949205287015737 2316 9 -2.1233717578048115 2316 10 -2.1056821237726773 2316 11 -2.4604100048928927 2316 12 -2.463769046376118 2316 13 -2.5126222285796196 2316 14 -2.899139087381627 2316 15 -3.227170776165867 2316 16 -2.811879542010331 2316 17 -2.791532334045719 2316 18 -2.4736265543751053 2316 19 -2.82103784991493 2316 20 -2.9079157339206674 2316 21 -3.0331811782672204 2316 22 -3.0727697526348976 2316 23 -2.9569302605235777 2316 24 -2.762102359882494 2316 25 -3.126742733638654 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 159027.0 3 4.0 4 1300.0 5 6331.0 6 7730.0 7 6887.0 8 6365.0 9 6220.0 10 5991.0 11 5532.0 12 5472.0 13 5611.0 14 5662.0 15 5824.0 16 6176.0 17 6647.0 18 7575.0 19 9523.0 20 12207.0 21 15845.0 22 19469.0 23 23160.0 24 26429.0 25 30086.0 26 34052.0 27 37987.0 28 43638.0 29 52951.0 30 64552.0 31 79986.0 32 101619.0 33 130215.0 34 170986.0 35 227583.0 36 321045.0 37 439338.0 38 897358.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5324605048984 18.502884593168393 12.673592101214076 25.291062800719132 2 16.599058200516804 21.497529861379284 38.294928033427645 23.608483904676266 3 18.50479325659167 24.55820301682671 29.884043674237365 27.052960052344254 4 12.727737868853392 16.930882609497843 37.888777159527095 32.45260236212167 5 13.680797138210337 37.40776049153776 35.37068085372841 13.5407615165235 6 31.800576148471244 36.82012655443056 18.18504190520774 13.194255391890458 7 27.984990538721927 31.72526765655979 22.313246492496106 17.976495312222177 8 25.700223716643002 35.67779847553901 20.694542901123672 17.92743490669432 9 26.627562506215714 15.430371790892192 19.9552770023135 37.98678870057859 10 15.314646513859742 28.61675813182703 34.08471050096388 21.983884853349352 11 34.50886909013345 22.52236233597633 24.096339953716587 18.872428620173633 12 23.124160564803645 24.63307619953636 31.19800775720998 21.044755478450018 13 28.78009953847179 21.563175252470966 25.779446239815858 23.877278969241384 14 21.740344645379313 21.222760444606067 27.423825682918483 29.61306922709614 15 23.566769567064906 29.19555194360536 23.597475608453436 23.6402028808763 16 23.16896392726586 27.728091139013316 26.40257461953139 22.70037031418944 17 22.438630569910536 27.745019808256217 27.10658820826947 22.709761413563776 18 22.321349940714235 27.464427704015193 28.17448398279792 22.03973837247265 19 23.732526468553502 26.826412231019958 27.187686471955626 22.253374828470914 20 23.754210783624323 26.933444504718086 27.3827175376208 21.929627174036796 21 23.786088843348804 26.491035969970333 26.802483274064254 22.920391912616616 22 23.614535937347224 26.836815605867205 27.06572273172326 22.482925725062316 23 23.380407237152287 26.675279135056364 27.013750482191078 22.930563145600274 24 23.2281690103115 26.54804790022951 27.44666274217496 22.77712034728403 25 23.408894106648113 26.560433327015765 27.194545105080326 22.836127461255796 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 531.0 1 531.0 2 671.0 3 811.0 4 811.0 5 811.0 6 1852.5 7 2894.0 8 2894.0 9 2894.0 10 4498.0 11 6102.0 12 6102.0 13 6102.0 14 9835.0 15 13568.0 16 13568.0 17 13568.0 18 23681.5 19 33795.0 20 33795.0 21 33795.0 22 51784.5 23 69774.0 24 69774.0 25 69774.0 26 97225.5 27 124677.0 28 124677.0 29 124677.0 30 160192.0 31 195707.0 32 195707.0 33 195707.0 34 226146.5 35 256586.0 36 256586.0 37 256586.0 38 285649.5 39 314713.0 40 314713.0 41 314713.0 42 339280.5 43 363848.0 44 363848.0 45 363848.0 46 375669.5 47 387491.0 48 387491.0 49 387491.0 50 382577.5 51 377664.0 52 377664.0 53 377664.0 54 346435.5 55 315207.0 56 315207.0 57 315207.0 58 273467.5 59 231728.0 60 231728.0 61 231728.0 62 190570.5 63 149413.0 64 149413.0 65 149413.0 66 115813.0 67 82213.0 68 82213.0 69 82213.0 70 59764.0 71 37315.0 72 37315.0 73 37315.0 74 26125.5 75 14936.0 76 14936.0 77 14936.0 78 10050.5 79 5165.0 80 5165.0 81 5165.0 82 3373.5 83 1582.0 84 1582.0 85 1582.0 86 1039.0 87 496.0 88 496.0 89 496.0 90 309.0 91 122.0 92 122.0 93 122.0 94 79.5 95 37.0 96 37.0 97 37.0 98 22.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.443401934715005 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 5.357651714465292E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 3.013679089386726E-4 15 0.0 16 0.0 17 6.697064643081615E-5 18 0.0 19 3.013679089386726E-4 20 1.0045596964622422E-4 21 1.0045596964622422E-4 22 6.362211410927533E-4 23 5.022798482311211E-4 24 3.013679089386726E-4 25 3.3485323215408074E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2986383.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.29377718701679 #Duplication Level Percentage of deduplicated Percentage of total 1 82.39335673684018 59.565269736235116 2 11.641916266477157 16.832762011972118 3 3.138877187936104 6.807638641261875 4 1.1487167201359922 3.3218028246604856 5 0.5553384340308983 2.0073756506608293 6 0.3060187246132548 1.3273949695347416 7 0.18487972200166486 0.9355957400150174 8 0.12900582675603758 0.7461054796262295 9 0.09537360302361236 0.620542620586082 >10 0.3807311532318502 4.521642316825421 >50 0.01617001734036285 0.7871235296352639 >100 0.007982416066771303 1.0890637349041434 >500 9.799149280834572E-4 0.4952175768701733 >1k 6.066140026367133E-4 0.7554092092987588 >5k 4.6662615587439484E-5 0.18705595791370594 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5545 0.18567611722943775 No Hit GGTATCAACGCAGAGTACTTTTTTT 3405 0.11401752554846448 No Hit TATCAACGCAGAGTACTTTTTTTTT 3288 0.11009974273226174 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.3485323215408074E-5 0.0 9 0.0 0.0 0.0 3.3485323215408074E-5 0.0 10 0.0 0.0 0.0 3.3485323215408074E-5 0.0 11 0.0 0.0 0.0 3.3485323215408074E-5 0.0 12 3.3485323215408074E-5 0.0 0.0 3.3485323215408074E-5 0.0 13 3.3485323215408074E-5 0.0 0.0 3.3485323215408074E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGTCG 55 0.003179341 12.034313 5 TCCAACG 210 0.0 11.256772 18 GGTATCA 1610 0.0 11.185912 1 TAGGACC 490 0.0 10.999291 4 GTATTAT 395 0.0 10.521511 1 GTATTAA 320 0.0 9.586004 1 GTATCAA 2955 0.0 9.543624 1 ACACCGT 120 1.7986358E-5 9.455531 6 GTCCTAA 765 0.0 9.442508 1 TTAGGAC 875 0.0 9.293436 3 TAGCACC 215 1.1641532E-10 9.235635 4 GTCCTAC 1130 0.0 9.194684 1 GTTCTAG 280 0.0 9.188429 1 AGGACGT 1180 0.0 9.135005 5 GTAGGAC 1185 0.0 9.09646 3 ACCGCAC 125 2.9123134E-5 9.07731 8 TAGGACA 595 0.0 9.05824 4 GTCCTAG 405 0.0 9.040088 1 GTCCAGG 620 0.0 8.937627 1 CGGTAGG 100 0.00200781 8.905708 1 >>END_MODULE