FastQCFastQC Report
Thu 26 May 2016
SRR1512094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986383
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65680.2199316028788002No Hit
TATCAACGCAGAGTACTTTTTTTTT44340.14847392313711938No Hit
GGTATCAACGCAGAGTACTTTTTTT43820.14673268632991815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG701.0895035E-412.2167424
GGTATCA17200.011.8686691
TAGGACC5850.011.207384
CTAGTAC1701.0913936E-1111.1787183
TAGGCGG600.005869018411.0855625
TTAGGAC7000.010.1806173
GACGTGA5750.010.0748857
GTCCTAC12200.09.9302591
GGACGTG10400.09.679456
GTAGGAC12900.09.6492263
GTATTAT3500.09.5393051
ATAGGAC3600.09.501913
AGGACGT10600.09.501915
ACCGCTC1201.7082617E-59.4968198
TAGGACA5600.09.3322334
TAGGACT4200.09.2756744
TCCAACG2054.2564352E-109.26549918
GATATAC6700.09.2545491
GTATCAA31400.09.1443651
CTAATAC2401.0913936E-119.1059973
Produced by FastQC (version 0.11.5)