FastQCFastQC Report
Thu 26 May 2016
SRR1512093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2420148
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68750.2840735359986249No Hit
TATCAACGCAGAGTACTTTTTTTTT43290.1788733581582614No Hit
GGTATCAACGCAGAGTACTTTTTTT41690.17226219222956612No Hit
GTCCTACAGTGGACATTTCTAAATT30450.12581875158048186No Hit
GTCCTAAAGTGTGTATTTCTCATTT28600.11817459097542794No Hit
CTGTAGGACGTGGAATATGGCAAGA26780.11065438973153709No Hit
CTTTAGGACGTGAAATATGGCGAGG24890.10284494997826578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.012.2200221
GTCTTAT1759.094947E-1211.3135911
TAGGACC6550.011.1152214
ATACGAG600.00608788911.03101511
GCGTGCG951.4342069E-510.9480749
AAGGCGT1605.9117156E-1010.637056
GTCCTAC17800.010.5667671
CTCAACG750.001880603510.559582
GGCGTGC1356.098708E-810.5059468
GCGCCAC1502.6328053E-810.085513
AGGACCT13200.010.0281955
GGACGTG17900.09.9833776
AGGACGT18300.09.76516155
GTAGGAC19100.09.7521763
AAAGGCG1752.7939677E-99.7253035
TCCTACA20600.09.6112682
CCTACAG21700.09.5858733
GACGTGG9100.09.5590597
GGCGAGG8950.09.50778119
GTCTTAC2501.8189894E-129.5034171
Produced by FastQC (version 0.11.5)