##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512093_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2420148 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.86543095711502 31.0 28.0 33.0 18.0 34.0 2 26.31550260562577 31.0 25.0 34.0 10.0 34.0 3 27.986962780788613 31.0 27.0 34.0 16.0 34.0 4 32.26681219495667 35.0 32.0 37.0 19.0 37.0 5 32.66917725692809 35.0 33.0 37.0 25.0 37.0 6 32.91497916656337 36.0 35.0 37.0 26.0 37.0 7 32.95443253883647 37.0 35.0 37.0 26.0 37.0 8 32.98823956220859 37.0 35.0 37.0 26.0 37.0 9 34.51101585522869 39.0 35.0 39.0 27.0 39.0 10 34.36418185995237 39.0 35.0 39.0 27.0 39.0 11 34.38051391898347 39.0 35.0 39.0 27.0 39.0 12 34.27667316213719 38.0 35.0 39.0 26.0 39.0 13 34.26593001750306 39.0 35.0 39.0 26.0 39.0 14 35.31287590676273 39.0 36.0 41.0 26.0 41.0 15 35.2431111651023 39.0 35.0 41.0 25.0 41.0 16 35.20625680743492 39.0 35.0 41.0 25.0 41.0 17 35.1470306774627 39.0 35.0 41.0 25.0 41.0 18 35.09859686267121 39.0 35.0 41.0 25.0 41.0 19 35.11766346520957 39.0 35.0 41.0 25.0 41.0 20 35.04330561602018 39.0 35.0 41.0 25.0 41.0 21 34.96924526929758 39.0 35.0 41.0 24.0 41.0 22 34.85417751311077 39.0 35.0 41.0 24.0 41.0 23 34.73914446554508 39.0 35.0 41.0 22.0 41.0 24 34.61979845860667 39.0 34.0 41.0 19.0 41.0 25 34.49037248961634 39.0 34.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5796391645875545 1101 2 3.048508367699963 1101 3 1.5092491356824347 1101 4 0.7847096297597957 1101 5 0.5797984950160995 1101 6 0.09611194091433362 1101 7 -0.0025283610095812037 1101 8 0.008993322071219723 1101 9 -0.24777128982297114 1101 10 -0.4964439009628592 1101 11 -0.1102430490407258 1101 12 -0.3907551028270504 1101 13 -0.2649379740399169 1101 14 -0.1435581819831171 1101 15 0.0470601516403093 1101 16 -0.01645573104024578 1101 17 -0.17005945588230276 1101 18 -0.21250314204937837 1101 19 -0.23089463882697459 1101 20 -0.16444257131986717 1101 21 -0.021592352401803794 1101 22 -0.15593518990969812 1101 23 -0.10902440979620565 1101 24 -0.15169389412633194 1101 25 -0.1696723720570361 1102 1 1.3781867708274689 1102 2 3.8798645115561072 1102 3 2.4329168141578066 1102 4 1.5025831024409584 1102 5 1.2154128806304811 1102 6 0.989900844318619 1102 7 0.8884988809791992 1102 8 0.7477977868372179 1102 9 0.9118671486616208 1102 10 0.883320565340064 1102 11 0.822593345334127 1102 12 0.7748341156467831 1102 13 0.8016300732973605 1102 14 0.8017897393263382 1102 15 0.9458488629290187 1102 16 0.8927312358390296 1102 17 0.7853313072007637 1102 18 0.8015164921625626 1102 19 0.8651919668750168 1102 20 0.9527287189283484 1102 21 0.8844535247979195 1102 22 1.0019646026054758 1102 23 1.018108073105509 1102 24 0.8623353458644516 1102 25 0.866210014786887 1103 1 0.9536653062311444 1103 2 2.7718827282387544 1103 3 1.7293176577643798 1103 4 1.0931381978360122 1103 5 0.7842002174547318 1103 6 0.5896997289652077 1103 7 0.5499925241455301 1103 8 0.3966153476965957 1103 9 0.409052171950151 1103 10 0.40783344920374986 1103 11 0.5714314006706331 1103 12 0.3897219704352608 1103 13 0.550691865918786 1103 14 0.5407826449751596 1103 15 0.3754850824142295 1103 16 0.23787383201414514 1103 17 0.5579759315490591 1103 18 0.38309337552700384 1103 19 0.6311489384011679 1103 20 0.6003237026746007 1103 21 0.6480408667280599 1103 22 0.6329282117892703 1103 23 0.6570354531596365 1103 24 0.5050914346388637 1103 25 0.6549715243150516 1104 1 1.4070099733402408 1104 2 3.8313687375741914 1104 3 2.3697898930219097 1104 4 1.7628931911301606 1104 5 1.4213715888647442 1104 6 1.0211826810967821 1104 7 0.9549734617435846 1104 8 1.055498663027322 1104 9 1.029086471778271 1104 10 1.0551215691483264 1104 11 1.00194162189446 1104 12 1.0896993051976906 1104 13 1.0071173392855641 1104 14 1.0379574734413097 1104 15 0.8749135921592455 1104 16 1.0950439608276668 1104 17 0.9917677529039466 1104 18 1.1417556266953213 1104 19 1.167750520316737 1104 20 0.9820163689950974 1104 21 0.969246504645163 1104 22 0.9363662818911536 1104 23 1.0541340597855182 1104 24 1.0777276204368889 1104 25 0.975638125265256 1105 1 1.174202452877779 1105 2 3.6543329705264114 1105 3 2.170906691943312 1105 4 1.4356267988965214 1105 5 1.0821660536567208 1105 6 0.776179362217917 1105 7 0.49617191882538236 1105 8 0.6011533913991798 1105 9 0.6431801633978509 1105 10 0.6526710779491651 1105 11 0.4554980978812324 1105 12 0.4966272718708211 1105 13 0.42726721724276473 1105 14 0.5860686641105133 1105 15 0.5525513035797971 1105 16 0.39707856164533695 1105 17 0.682804981852982 1105 18 0.5661510911712782 1105 19 0.6549059586894899 1105 20 0.7634719700633141 1105 21 0.6194458324555683 1105 22 0.8932875225971415 1105 23 0.7827613874324015 1105 24 0.8880773995656313 1105 25 0.6563731742375865 1106 1 0.4229395423706883 1106 2 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0.2050699972633936 1107 14 0.3491204476773717 1107 15 0.34652072992213334 1107 16 0.36452727559042586 1107 17 0.30859919117012424 1107 18 0.3159874735526387 1107 19 0.32010811489598723 1107 20 0.3744084740078648 1107 21 0.36833572470244036 1107 22 0.3386207722628427 1107 23 0.5061292189584634 1107 24 0.6734877247729045 1107 25 0.7042655423601545 1108 1 0.5075489654883398 1108 2 2.199319067192551 1108 3 1.31241130573504 1108 4 0.7909540957994423 1108 5 0.4757675788526825 1108 6 0.4177570245645086 1108 7 0.25289097954794215 1108 8 0.4027805178233308 1108 9 0.24917458084888722 1108 10 0.25448573584485956 1108 11 0.37295564172362106 1108 12 0.3743406158408007 1108 13 0.4162956296750693 1108 14 0.30225646495363634 1108 15 0.2191568955471297 1108 16 0.2930903677061636 1108 17 0.15610497161493697 1108 18 0.29697946178347934 1108 19 0.2897893518197776 1108 20 0.2990045068861349 1108 21 0.4804194049509931 1108 22 0.3831917944348433 1108 23 0.5178800949503284 1108 24 0.5125192692293297 1108 25 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-1.840093206450348 2315 20 -1.67482781359314 2315 21 -1.7110876814415903 2315 22 -1.9318743075063338 2315 23 -1.7970024124009853 2315 24 -1.7396222253998843 2315 25 -1.611215484085875 2316 1 -1.491358539997936 2316 2 -1.8332811214368547 2316 3 -1.2492152053200734 2316 4 -1.661658410596793 2316 5 -1.5887676716416692 2316 6 -1.6751273160119489 2316 7 -1.6707108464630593 2316 8 -1.7607412372625788 2316 9 -1.9638138350639878 2316 10 -2.0776174983578315 2316 11 -2.4316544620551923 2316 12 -2.4126783132044594 2316 13 -2.526308283120148 2316 14 -2.740509417770234 2316 15 -2.8761802529932226 2316 16 -2.7183587898135357 2316 17 -2.6433418986303963 2316 18 -2.4858743319282013 2316 19 -2.7113102156165994 2316 20 -2.856950445125449 2316 21 -2.9118995642739023 2316 22 -2.823887466640606 2316 23 -2.8022195734112145 2316 24 -2.6252399091799816 2316 25 -2.9778751425689656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104179.0 3 3.0 4 909.0 5 4383.0 6 5109.0 7 4688.0 8 4239.0 9 4103.0 10 3945.0 11 3848.0 12 3805.0 13 3827.0 14 3933.0 15 4118.0 16 4254.0 17 4765.0 18 5420.0 19 6621.0 20 8686.0 21 11042.0 22 13672.0 23 16145.0 24 18966.0 25 22061.0 26 24846.0 27 28042.0 28 32331.0 29 39390.0 30 48747.0 31 61866.0 32 79645.0 33 102817.0 34 136510.0 35 186616.0 36 268414.0 37 371083.0 38 777120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42757054527244 19.433315648464475 13.172913392073543 24.966200414189547 2 15.831850508789438 21.968299785374395 39.74631694936592 22.45353275647024 3 18.117610989080006 25.460674305868896 30.436485702527282 25.985229002523813 4 12.213674535606915 17.649333842393112 39.041455315955886 31.095536306044092 5 12.940613549254012 38.07283686782792 36.02759004821193 12.95895953470614 6 30.42627971512486 37.79479602073923 18.86132583627117 12.917598427864743 7 26.80600525257133 32.25546536823368 23.2085393124718 17.729990066723193 8 24.488335385556205 36.88108952374656 21.37446593998694 17.256109150710287 9 26.085512125704707 15.7546150070161 21.02809414961399 37.1317787176652 10 15.03945213267949 29.166728646347252 34.61432937159215 21.17948984938111 11 33.11512353789933 23.12313131263047 25.28308186110932 18.478663288360877 12 22.88628629323496 25.112596419723094 32.19365096680038 19.80746632024157 13 28.31471463728665 22.510730748698013 26.32070435361804 22.853850260397298 14 20.87552967086547 22.442425732448807 28.30970173151498 28.372342865170747 15 22.64721000533852 30.87749178975831 24.220708816155046 22.254589388748126 16 21.732059361658873 28.494414391185995 28.146295185253134 21.627231061902 17 21.291507792085444 28.61647304214453 28.40669248326962 21.68532668250041 18 21.077967132588586 27.87618773727888 30.172906780907617 20.872938349224924 19 22.88070461922927 27.271765647002823 28.292407393940195 21.55512233982771 20 22.900261843598756 27.708730089535884 28.738832806436964 20.652175260428397 21 22.65088746638635 27.17247044395632 27.901434127169082 22.275207962488246 22 22.659515565215234 28.080854826580282 28.07870619055096 21.180923417653524 23 22.029561111985714 27.857449510131442 28.308911182374235 21.804078195508605 24 22.41869805321582 27.226219916575246 28.854593423121344 21.500488607087593 25 22.332642466493784 27.549306901891953 28.528461895718777 21.58958873589549 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1265.0 1 1265.0 2 1166.5 3 1068.0 4 1068.0 5 1068.0 6 2391.5 7 3715.0 8 3715.0 9 3715.0 10 5230.0 11 6745.0 12 6745.0 13 6745.0 14 10792.0 15 14839.0 16 14839.0 17 14839.0 18 25377.0 19 35915.0 20 35915.0 21 35915.0 22 53912.5 23 71910.0 24 71910.0 25 71910.0 26 100339.0 27 128768.0 28 128768.0 29 128768.0 30 162958.5 31 197149.0 32 197149.0 33 197149.0 34 219170.0 35 241191.0 36 241191.0 37 241191.0 38 257788.5 39 274386.0 40 274386.0 41 274386.0 42 288054.5 43 301723.0 44 301723.0 45 301723.0 46 303139.5 47 304556.0 48 304556.0 49 304556.0 50 292512.5 51 280469.0 52 280469.0 53 280469.0 54 249615.5 55 218762.0 56 218762.0 57 218762.0 58 186290.5 59 153819.0 60 153819.0 61 153819.0 62 124463.0 63 95107.0 64 95107.0 65 95107.0 66 73396.0 67 51685.0 68 51685.0 69 51685.0 70 37536.0 71 23387.0 72 23387.0 73 23387.0 74 16204.0 75 9021.0 76 9021.0 77 9021.0 78 6171.0 79 3321.0 80 3321.0 81 3321.0 82 2146.5 83 972.0 84 972.0 85 972.0 86 622.5 87 273.0 88 273.0 89 273.0 90 174.0 91 75.0 92 75.0 93 75.0 94 48.0 95 21.0 96 21.0 97 21.0 98 13.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.490675776853316 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.3055829643476346E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 5.371572317064907E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 1.6527914821738173E-4 20 4.131978705434543E-5 21 0.0 22 3.3055829643476346E-4 23 3.7187808348910893E-4 24 1.239593611630363E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2420148.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.41694273493326 #Duplication Level Percentage of deduplicated Percentage of total 1 82.00761290623252 58.56732994752982 2 12.173447735509415 17.387808396271577 3 3.1844857898980616 6.822787178920757 4 1.1578035019705744 3.307471455941508 5 0.5066180534313646 1.809055625519556 6 0.2700917469757312 1.1573476096166326 7 0.16100713483408233 0.8049046129852935 8 0.10779419303557489 0.6158665368943994 9 0.07484913936142765 0.48109470295807055 >10 0.32440244495921666 3.8794317689045297 >50 0.0170094360719523 0.8500593170451974 >100 0.01189545103927413 1.6637566948208462 >500 0.0018128719040687617 0.850156973198851 >1k 0.0011111150379662573 1.515798643399061 >5k 5.847973884032933E-5 0.2871305359940636 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6875 0.2840735359986249 No Hit TATCAACGCAGAGTACTTTTTTTTT 4329 0.1788733581582614 No Hit GGTATCAACGCAGAGTACTTTTTTT 4169 0.17226219222956612 No Hit GTCCTACAGTGGACATTTCTAAATT 3045 0.12581875158048186 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2860 0.11817459097542794 No Hit CTGTAGGACGTGGAATATGGCAAGA 2678 0.11065438973153709 No Hit CTTTAGGACGTGAAATATGGCGAGG 2489 0.10284494997826578 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.131978705434544E-5 2 0.0 0.0 0.0 0.0 4.131978705434544E-5 3 0.0 0.0 0.0 4.131978705434544E-5 4.131978705434544E-5 4 0.0 0.0 0.0 4.131978705434544E-5 8.263957410869088E-5 5 0.0 0.0 0.0 4.131978705434544E-5 8.263957410869088E-5 6 0.0 0.0 0.0 4.131978705434544E-5 8.263957410869088E-5 7 0.0 0.0 0.0 8.263957410869088E-5 8.263957410869088E-5 8 0.0 0.0 0.0 8.263957410869088E-5 1.239593611630363E-4 9 0.0 0.0 0.0 1.239593611630363E-4 1.239593611630363E-4 10 0.0 0.0 0.0 1.239593611630363E-4 1.239593611630363E-4 11 0.0 0.0 0.0 2.479187223260726E-4 1.239593611630363E-4 12 0.0 0.0 0.0 2.479187223260726E-4 2.479187223260726E-4 13 0.0 0.0 0.0 2.8923850938041804E-4 2.479187223260726E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1685 0.0 12.220022 1 GTCTTAT 175 9.094947E-12 11.313591 1 TAGGACC 655 0.0 11.115221 4 ATACGAG 60 0.006087889 11.031015 11 GCGTGCG 95 1.4342069E-5 10.948074 9 AAGGCGT 160 5.9117156E-10 10.63705 6 GTCCTAC 1780 0.0 10.566767 1 CTCAACG 75 0.0018806035 10.55958 2 GGCGTGC 135 6.098708E-8 10.505946 8 GCGCCAC 150 2.6328053E-8 10.0855 13 AGGACCT 1320 0.0 10.028195 5 GGACGTG 1790 0.0 9.983377 6 AGGACGT 1830 0.0 9.7651615 5 GTAGGAC 1910 0.0 9.752176 3 AAAGGCG 175 2.7939677E-9 9.725303 5 TCCTACA 2060 0.0 9.611268 2 CCTACAG 2170 0.0 9.585873 3 GACGTGG 910 0.0 9.559059 7 GGCGAGG 895 0.0 9.507781 19 GTCTTAC 250 1.8189894E-12 9.503417 1 >>END_MODULE