FastQCFastQC Report
Thu 26 May 2016
SRR1512093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2420148
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80830.33398783876027416No Hit
TATCAACGCAGAGTACTTTTTTTTT55690.23010989410564972No Hit
GGTATCAACGCAGAGTACTTTTTTT54200.22395324583455228No Hit
GTCCTACAGTGGACATTTCTAAATT32680.13503306409360089No Hit
GTCCTAAAGTGTGTATTTCTCATTT30970.12796738050730783No Hit
CTGTAGGACGTGGAATATGGCAAGA28950.11962078352233003No Hit
CTTTAGGACGTGAAATATGGCGAGG28020.11577804332627592No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26190.1082165222953307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAC400.005292433314.2450418
TGCGCAT853.9630922E-612.28933110
TAGGACC6550.011.606454
GGTATCA18700.011.2215021
GCGCATT600.0058943211.07901911
GCCGGTT1252.2536733E-710.63585911
AATGTCC5800.010.4791118
GGCGAGG9950.010.21294719
AATCCCG2700.010.20057419
GTGCGCC1409.750147E-810.17460911
ACCGTTC750.002656139410.1298078
CGTGCGC1054.1243817E-59.94850610
GCGTGCG1252.5840345E-69.8761549
TAATACT3850.09.8730194
AGGACCT13350.09.8230995
TTAGGAC13900.09.5711473
ACTATAC2106.548362E-119.5027813
TTACACG900.0011110079.5027814
GGACGGG1304.2592037E-69.4966946
GGACGTG18000.09.4439346
Produced by FastQC (version 0.11.5)