##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512093_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2420148 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.940954850695082 31.0 31.0 34.0 28.0 34.0 2 31.132531977383202 31.0 31.0 34.0 28.0 34.0 3 31.23353034607801 31.0 31.0 34.0 27.0 34.0 4 34.81855655108696 37.0 35.0 37.0 32.0 37.0 5 34.44590124240336 37.0 35.0 37.0 30.0 37.0 6 34.38734821176226 37.0 35.0 37.0 30.0 37.0 7 34.22462965074863 37.0 35.0 37.0 30.0 37.0 8 34.13915801843524 37.0 35.0 37.0 30.0 37.0 9 35.64566753768778 39.0 35.0 39.0 30.0 39.0 10 35.461969681193054 39.0 35.0 39.0 30.0 39.0 11 35.45444906675129 39.0 35.0 39.0 30.0 39.0 12 35.28683659015895 39.0 35.0 39.0 29.0 39.0 13 35.258382545199716 39.0 35.0 39.0 29.0 39.0 14 36.291336314969165 40.0 36.0 41.0 29.0 41.0 15 36.22567380176749 40.0 36.0 41.0 29.0 41.0 16 36.121333075497866 39.0 36.0 41.0 28.0 41.0 17 36.067687595965204 39.0 36.0 41.0 28.0 41.0 18 36.00923373281304 39.0 36.0 41.0 28.0 41.0 19 36.03028451152574 39.0 36.0 41.0 27.0 41.0 20 35.9225997748898 39.0 36.0 41.0 27.0 41.0 21 35.8031384857455 39.0 36.0 41.0 27.0 41.0 22 35.69294026646304 39.0 36.0 41.0 27.0 41.0 23 35.76153855053493 39.0 36.0 41.0 27.0 41.0 24 35.65403066258758 39.0 36.0 41.0 27.0 41.0 25 35.232578338184275 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06724860134527688 1101 2 0.0064856309009861945 1101 3 0.1140641162953493 1101 4 0.020615222973262348 1101 5 0.0981725361837178 1101 6 -0.01769995019426318 1101 7 -0.05625721954213958 1101 8 -0.15453648963212885 1101 9 -0.18965972420591726 1101 10 -0.0545959734256769 1101 11 -0.23267777571060577 1101 12 -0.3962173220278302 1101 13 -0.14946531757458814 1101 14 -0.32311929755282875 1101 15 -0.3239585006115888 1101 16 -0.47577793317538664 1101 17 -0.31581072044601655 1101 18 -0.31258851717277025 1101 19 -0.352412161311598 1101 20 -0.4007219639198567 1101 21 -0.43766018516097205 1101 22 -0.5453076875498297 1101 23 -0.46814305921990496 1101 24 -0.6950782248633232 1101 25 -0.7052319842859518 1102 1 0.1901257242223977 1102 2 0.16874644700795827 1102 3 0.20503564476687686 1102 4 0.20031052766856305 1102 5 0.17579683496186504 1102 6 0.19825236671959345 1102 7 0.24058536095429162 1102 8 0.33750031971236893 1102 9 0.24615926449768466 1102 10 0.43892973163849547 1102 11 0.38437824519157004 1102 12 0.6525512171253283 1102 13 0.5725050197803654 1102 14 0.673874093671337 1102 15 0.5421753655222759 1102 16 0.40370796554127963 1102 17 0.5570327822051766 1102 18 0.501337940984456 1102 19 0.38133486089696333 1102 20 0.5317253964121207 1102 21 0.5660716599170215 1102 22 0.5078218751951127 1102 23 0.676755311640008 1102 24 0.647583920683438 1102 25 0.5990070459146182 1103 1 0.24634760636709885 1103 2 0.3092910469614374 1103 3 0.36380011854813077 1103 4 0.33458481043278 1103 5 0.39481507150075146 1103 6 0.376501485953483 1103 7 0.39235121899918823 1103 8 0.4552513735114374 1103 9 0.52256806478362 1103 10 0.43430145464960646 1103 11 0.3677117676319597 1103 12 0.49414734686033057 1103 13 0.33343056171390373 1103 14 0.4639606526982618 1103 15 0.4302478634941025 1103 16 0.573035250285443 1103 17 0.6155703984351035 1103 18 0.574608178748754 1103 19 0.5056714497082524 1103 20 0.6344396027014412 1103 21 0.6333024092915522 1103 22 0.5834440137689825 1103 23 0.5765546926926106 1103 24 0.6868209162640113 1103 25 0.3821864697432389 1104 1 0.27601459745094914 1104 2 0.1791582210332372 1104 3 0.1574150816312141 1104 4 0.1897511656404376 1104 5 0.22850481251309418 1104 6 0.12861233805534766 1104 7 0.061007833361102826 1104 8 0.1834674075877345 1104 9 0.26672855549121977 1104 10 0.15582569014038228 1104 11 0.32042437658476075 1104 12 0.2574476414737106 1104 13 0.32914481866897205 1104 14 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2314 21 -0.49648530802650725 2314 22 -0.5443651270295291 2314 23 -0.20937819686225367 2314 24 -0.33430138390797026 2314 25 -0.5500163454485332 2315 1 -0.48184445707818924 2315 2 -0.4899231926208998 2315 3 -0.5861892298828053 2315 4 -0.5703255322100844 2315 5 -0.47873039074665513 2315 6 -0.6419894230704486 2315 7 -0.5956304652134037 2315 8 -0.6366084334219693 2315 9 -0.8352368647748278 2315 10 -0.7748648191061562 2315 11 -0.6949397179396186 2315 12 -0.8160014804234024 2315 13 -0.9003359251960248 2315 14 -0.9052848908325828 2315 15 -1.0514845420783558 2315 16 -0.9656626368361643 2315 17 -0.8490289429479887 2315 18 -0.8599828992268712 2315 19 -0.8517308996533899 2315 20 -0.9491150499775927 2315 21 -0.7633980906875735 2315 22 -0.8182559705145778 2315 23 -0.8713669621007796 2315 24 -0.8052854115661177 2315 25 -1.0388226298277488 2316 1 -1.146812147066619 2316 2 -1.098195947063033 2316 3 -1.136679891605148 2316 4 -1.0396327351754593 2316 5 -1.4067852922473918 2316 6 -1.378390309453323 2316 7 -1.5340337369178911 2316 8 -1.4192898660267304 2316 9 -1.850135221990577 2316 10 -1.9681842935025458 2316 11 -1.781135043633519 2316 12 -1.9895486090614014 2316 13 -1.977229156788809 2316 14 -1.9893566861184624 2316 15 -2.018294698764585 2316 16 -2.2579671180363334 2316 17 -2.0622574988584432 2316 18 -2.1468048733356966 2316 19 -2.010061763617692 2316 20 -2.062205041910566 2316 21 -2.104768363242435 2316 22 -2.377749101098118 2316 23 -2.335219208261705 2316 24 -2.263643724249434 2316 25 -2.2954437763107194 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34656.0 3 0.0 4 628.0 5 5819.0 6 7251.0 7 5813.0 8 4807.0 9 4219.0 10 4064.0 11 3787.0 12 3707.0 13 3511.0 14 3532.0 15 3498.0 16 3701.0 17 3747.0 18 4157.0 19 4642.0 20 5635.0 21 7407.0 22 9450.0 23 11857.0 24 14925.0 25 17911.0 26 20774.0 27 23819.0 28 27728.0 29 33994.0 30 43756.0 31 56280.0 32 72379.0 33 94095.0 34 123686.0 35 164633.0 36 239124.0 37 414990.0 38 936166.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.289387376866785 20.137802129488186 13.872631590741868 24.700178902903158 2 14.68906033845864 21.479140945099225 40.56702317378937 23.264775542652764 3 17.993403709194645 25.791645800174205 30.912778887902725 25.30217160272843 4 12.588238405254554 18.19731685830784 38.76597629566456 30.44846844077304 5 12.893055499283532 38.2086037491225 36.09169152052707 12.8066492310669 6 30.425742557893155 38.12804010333252 18.635554519806227 12.810662818968096 7 26.86050605169601 32.33132849726545 23.1686243981773 17.63954105286123 8 24.697405196878766 36.94441643788522 21.22365983104607 17.13451853418995 9 26.123030492350054 15.546983077068013 20.900622606551337 37.429363824030595 10 14.937309619081146 29.406135492540127 34.59358683849087 21.06296804988786 11 33.22003447723032 23.051358842517068 25.2158545675719 18.51275211268071 12 22.803109561894562 25.24353882489831 32.12807646474513 19.825275148461998 13 28.40582476774148 22.405654530218815 26.39367509755602 22.794845604483694 14 20.826990746020492 22.482798572649276 28.301120427345765 28.389090253984467 15 22.674398425220275 30.93628984673665 24.1827359318521 22.20657579619098 16 21.775486457852992 28.511810021535872 28.080100886392074 21.632602634219065 17 21.336133162104133 28.702087640921132 28.328680725311013 21.63309847166372 18 21.099288142708627 27.88333606043928 30.12964496386171 20.88773083299038 19 22.908659229649782 27.324095323174717 28.301680972966093 21.465564474209405 20 22.984792665572517 27.73404766981193 28.69072469948119 20.590434965134364 21 22.73282460411512 27.074831787146902 27.95019147589321 22.242152132844765 22 22.699396896388155 27.970024973679298 28.16509568836286 21.16548244156969 23 22.05522141621091 27.837223178086628 28.374256450431957 21.733298955270506 24 22.485737685815042 27.202074037588215 28.883551913336035 21.428636363260708 25 22.36114594649093 27.57362982637588 28.59589614203965 21.469328085093547 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1286.0 1 1286.0 2 1124.0 3 962.0 4 962.0 5 962.0 6 2263.0 7 3564.0 8 3564.0 9 3564.0 10 5004.0 11 6444.0 12 6444.0 13 6444.0 14 10447.5 15 14451.0 16 14451.0 17 14451.0 18 24990.5 19 35530.0 20 35530.0 21 35530.0 22 53946.0 23 72362.0 24 72362.0 25 72362.0 26 101650.0 27 130938.0 28 130938.0 29 130938.0 30 164051.5 31 197165.0 32 197165.0 33 197165.0 34 219893.5 35 242622.0 36 242622.0 37 242622.0 38 259344.5 39 276067.0 40 276067.0 41 276067.0 42 289155.0 43 302243.0 44 302243.0 45 302243.0 46 303381.0 47 304519.0 48 304519.0 49 304519.0 50 292923.5 51 281328.0 52 281328.0 53 281328.0 54 249752.0 55 218176.0 56 218176.0 57 218176.0 58 185373.0 59 152570.0 60 152570.0 61 152570.0 62 123086.5 63 93603.0 64 93603.0 65 93603.0 66 72361.5 67 51120.0 68 51120.0 69 51120.0 70 36749.0 71 22378.0 72 22378.0 73 22378.0 74 15470.5 75 8563.0 76 8563.0 77 8563.0 78 5838.5 79 3114.0 80 3114.0 81 3114.0 82 1982.0 83 850.0 84 850.0 85 850.0 86 543.5 87 237.0 88 237.0 89 237.0 90 143.5 91 50.0 92 50.0 93 50.0 94 28.0 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4322462923755076 2 0.0 3 0.0 4 0.0 5 0.05545115422693157 6 0.0 7 0.0 8 0.003305582964347635 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 8.263957410869086E-5 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005536851465282288 25 0.0014461925469020902 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2420148.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.72699510024928 #Duplication Level Percentage of deduplicated Percentage of total 1 80.25132577262272 55.154324731635505 2 13.169443414348756 18.10192546021914 3 3.5207961324056862 7.259212156224666 4 1.3230237627223307 3.6370979063252395 5 0.6064441691861522 2.0839542722115714 6 0.3201843229152047 1.3203183835301942 7 0.18564081126595372 0.8930974588397049 8 0.1327285031256857 0.7297624947185938 9 0.08467077796117693 0.5237251327864891 >10 0.36896747877769154 4.29327902854002 >50 0.019256501178610642 0.9172447187031043 >100 0.014032693851385536 1.8823295254813965 >500 0.002163512879180336 1.0309293907218808 >1k 0.001141854019557398 1.385063549208374 >5k 1.802927399301155E-4 0.7877357908541214 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8083 0.33398783876027416 No Hit TATCAACGCAGAGTACTTTTTTTTT 5569 0.23010989410564972 No Hit GGTATCAACGCAGAGTACTTTTTTT 5420 0.22395324583455228 No Hit GTCCTACAGTGGACATTTCTAAATT 3268 0.13503306409360089 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3097 0.12796738050730783 No Hit CTGTAGGACGTGGAATATGGCAAGA 2895 0.11962078352233003 No Hit CTTTAGGACGTGAAATATGGCGAGG 2802 0.11577804332627592 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2619 0.1082165222953307 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.131978705434544E-5 7 0.0 0.0 0.0 0.0 4.131978705434544E-5 8 0.0 0.0 0.0 0.0 4.131978705434544E-5 9 0.0 0.0 0.0 4.131978705434544E-5 4.131978705434544E-5 10 0.0 0.0 0.0 4.131978705434544E-5 4.131978705434544E-5 11 0.0 0.0 0.0 8.263957410869088E-5 4.131978705434544E-5 12 0.0 0.0 0.0 8.263957410869088E-5 4.131978705434544E-5 13 0.0 0.0 0.0 8.263957410869088E-5 4.131978705434544E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGAAC 40 0.0052924333 14.245041 8 TGCGCAT 85 3.9630922E-6 12.289331 10 TAGGACC 655 0.0 11.60645 4 GGTATCA 1870 0.0 11.221502 1 GCGCATT 60 0.00589432 11.079019 11 GCCGGTT 125 2.2536733E-7 10.635859 11 AATGTCC 580 0.0 10.479111 8 GGCGAGG 995 0.0 10.212947 19 AATCCCG 270 0.0 10.200574 19 GTGCGCC 140 9.750147E-8 10.174609 11 ACCGTTC 75 0.0026561394 10.129807 8 CGTGCGC 105 4.1243817E-5 9.948506 10 GCGTGCG 125 2.5840345E-6 9.876154 9 TAATACT 385 0.0 9.873019 4 AGGACCT 1335 0.0 9.823099 5 TTAGGAC 1390 0.0 9.571147 3 ACTATAC 210 6.548362E-11 9.502781 3 TTACACG 90 0.001111007 9.502781 4 GGACGGG 130 4.2592037E-6 9.496694 6 GGACGTG 1800 0.0 9.443934 6 >>END_MODULE