FastQCFastQC Report
Thu 26 May 2016
SRR1512092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666831
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72590.27219572593838903No Hit
GGTATCAACGCAGAGTACTTTTTTT48590.18220127184662244No Hit
TATCAACGCAGAGTACTTTTTTTTT47560.17833900985851747No Hit
GTCCTACAGTGGACATTTCTAAATT42220.15831524382309942No Hit
GTCCTAAAGTGTGTATTTCTCATTT38800.14549103411502265No Hit
CTTTAGGACGTGAAATATGGCGAGG38350.14380363810080204No Hit
CTGTAGGACGTGGAATATGGCAAGA37540.1407663252752049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG402.861645E-416.547965
CGTTAGG756.1389983E-714.5068551
GGTATCA16150.012.5551861
TAGGACC8950.012.3614444
GTCAACG1052.1375654E-611.3040432
CCATGCG803.9379814E-410.6379749
GGCGAAA1002.5258112E-510.40177119
GGCGAGG11550.010.15206119
GTCCTAA14300.010.0985411
ATAGGAC3550.09.8555263
ATTATAC3300.09.7425223
GGACGTG25650.09.7324686
AGGACGT26250.09.6901255
TTAGGAC16650.09.6547523
TTTAGAA10150.09.5499672
GTCCTAC23950.09.5399991
GTAGGAC26900.09.491133
TGGCGAA1201.7970044E-59.45615418
ACCGACC800.00469362279.45597658
AGGACCT17750.09.429345
Produced by FastQC (version 0.11.5)