FastQCFastQC Report
Thu 26 May 2016
SRR1512092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666831
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92100.34535371757715433No Hit
TATCAACGCAGAGTACTTTTTTTTT64970.24362248676425313No Hit
GGTATCAACGCAGAGTACTTTTTTT62210.2332731245437No Hit
GTCCTACAGTGGACATTTCTAAATT44880.16828962915160353No Hit
CTTTAGGACGTGAAATATGGCGAGG43730.16397739489303972No Hit
GTCCTAAAGTGTGTATTTCTCATTT42960.1610900728242622No Hit
CTGTAGGACGTGGAATATGGCAAGA40290.1510781898065532No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29560.11084316928969251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17850.012.4558571
GGCGAGG13800.011.49221319
TAGGACC8900.011.1045984
TTAGGAC19100.010.2990343
TGGCGAG27650.010.16629918
AGGACGT27850.010.1342325
CGGACTG856.577285E-410.0619735
ACTGCGT951.6514731E-49.9960038
GGACGTG27500.09.9796476
ACGTGAA17950.09.7342698
GATATAC6550.09.6152051
TAGAAAT8700.09.6122044
GTATCAA34550.09.5837921
GACGTGA16450.09.5827957
CGTGAAA18150.09.5745059
TATGGCG29700.09.55997116
AGGACCT18200.09.5029745
GACTGCG1002.7675874E-49.4962037
ATGGCGA30550.09.48048317
GTCCTAA14100.09.4746871
Produced by FastQC (version 0.11.5)