FastQCFastQC Report
Thu 26 May 2016
SRR1512091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175162
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47470.40394430725295744No Hit
GGTATCAACGCAGAGTACTTTTTTT33870.2882155821920722No Hit
TATCAACGCAGAGTACTTTTTTTTT31960.2719625038930803No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15350.1306202889473962No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13860.11794118598116685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCG500.001552312613.23864655
GGTATCA12500.011.6301441
TAATACC1002.0383177E-611.3474114
CTATACT1002.0383177E-611.3474114
CTAGGAC1701.2732926E-1111.1249123
TGTACCG600.006079852611.0322055
CCCTAGG1001.5370491E-510.8785371
TAAGGCT1159.347914E-710.68958954
ATAATAC1252.3844223E-710.5909183
CTCGAAC750.002750214710.08658818
TAGGACC750.002750214710.0865884
CCTAGGA1708.609277E-99.8895792
GTACATA2900.09.5485591
TCTATAC1107.185167E-59.4561763
GCTATAC800.00468951749.4561753
TTATACT1607.111885E-89.4561754
TAGATTG800.00468951749.4561755
GGACTAG950.0012635229.3690741
GTTATAA850.0053416899.3078381
GTATATA2052.0027073E-99.1659511
Produced by FastQC (version 0.11.5)