##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512091_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1175162 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.254794658098202 31.0 30.0 34.0 23.0 34.0 2 26.676206344316782 31.0 26.0 34.0 10.0 34.0 3 28.3571941570609 31.0 28.0 34.0 16.0 34.0 4 32.712634513369224 35.0 33.0 37.0 25.0 37.0 5 33.11396130916418 35.0 33.0 37.0 27.0 37.0 6 33.37051827748004 36.0 35.0 37.0 28.0 37.0 7 33.42506394863006 37.0 35.0 37.0 28.0 37.0 8 33.46663438742914 37.0 35.0 37.0 28.0 37.0 9 35.02987673188888 39.0 35.0 39.0 28.0 39.0 10 34.910739966064256 39.0 35.0 39.0 28.0 39.0 11 34.93537571841159 39.0 35.0 39.0 28.0 39.0 12 34.83000811802968 39.0 35.0 39.0 27.0 39.0 13 34.81688907571892 39.0 35.0 39.0 27.0 39.0 14 35.907199177645296 40.0 36.0 41.0 27.0 41.0 15 35.82063494224626 40.0 36.0 41.0 27.0 41.0 16 35.775024209428146 40.0 36.0 41.0 27.0 41.0 17 35.72450691904606 40.0 36.0 41.0 27.0 41.0 18 35.67045054213802 40.0 36.0 41.0 27.0 41.0 19 35.70009922036281 40.0 36.0 41.0 27.0 41.0 20 35.62428499219682 40.0 36.0 41.0 27.0 41.0 21 35.55656666910605 40.0 36.0 41.0 27.0 41.0 22 35.4474412889457 40.0 36.0 41.0 27.0 41.0 23 35.34146185802468 39.0 36.0 41.0 26.0 41.0 24 35.216301241871335 39.0 35.0 41.0 26.0 41.0 25 35.080382109019865 39.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.671985868402988 1101 2 3.2453516437939918 1101 3 1.7019698924158213 1101 4 1.0022939488615776 1101 5 0.7795072245048544 1101 6 0.39087841754908936 1101 7 0.36627766649834115 1101 8 0.2716362403407757 1101 9 0.3654918851427027 1101 10 0.11241914691571964 1101 11 0.2714296902844353 1101 12 -0.060447891445022606 1101 13 -0.13843626807238962 1101 14 0.1317886379390245 1101 15 0.34202163921128914 1101 16 0.13149330673348913 1101 17 0.26076094672968253 1101 18 0.13812829973247887 1101 19 0.20432034463930648 1101 20 0.17390274871713984 1101 21 0.2517224925809458 1101 22 0.16731191152249636 1101 23 -0.13895001733298074 1101 24 0.03734601098893364 1101 25 -0.04356816360374438 1102 1 1.3264884976755589 1102 2 3.736833896204157 1102 3 2.3223303218636744 1102 4 1.5121646762936507 1102 5 1.2223424567572607 1102 6 0.8891255691704743 1102 7 0.8792651774536466 1102 8 0.6998680545388467 1102 9 0.6754666879472566 1102 10 0.5737414519113386 1102 11 0.9731441951047728 1102 12 0.7086855441378006 1102 13 0.9366897179048266 1102 14 0.7311587080529591 1102 15 0.7902633131814909 1102 16 0.7822437449455819 1102 17 0.6130725155289767 1102 18 0.8327711568753315 1102 19 0.8702493542799701 1102 20 0.9451396023543026 1102 21 0.8514650429753345 1102 22 0.9030754960974363 1102 23 0.650209710975524 1102 24 0.7201516639249519 1102 25 0.8035834577810093 1103 1 0.9831519613461914 1103 2 3.2036670482690326 1103 3 1.8557241953418107 1103 4 1.1920744996360995 1103 5 0.8686208217509801 1103 6 0.6688474364819612 1103 7 0.6138142413692549 1103 8 0.38973004412735435 1103 9 0.40022725519433777 1103 10 0.40393162540109273 1103 11 0.5797881240193732 1103 12 0.48616355433810554 1103 13 0.6054858489671986 1103 14 0.5532709959424622 1103 15 0.30922258585501083 1103 16 0.3242751483858086 1103 17 0.3984330637692679 1103 18 0.5066093668581217 1103 19 0.4828487439508322 1103 20 0.6678463803866777 1103 21 0.7574131122022862 1103 22 0.5477013263245638 1103 23 0.6604153010835461 1103 24 0.45486967708187365 1103 25 0.48758502056148956 1104 1 1.2017138476776026 1104 2 3.542238209301022 1104 3 2.1659302304370414 1104 4 1.520098661420171 1104 5 0.9637102839669822 1104 6 0.5697582596413682 1104 7 0.6037607037546948 1104 8 0.6303119126822523 1104 9 0.6194966347109201 1104 10 0.5951896255735036 1104 11 0.7276735220140225 1104 12 0.5133693427025179 1104 13 0.6097055404644962 1104 14 0.519669528637273 1104 15 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1107 14 0.8187233378544931 1107 15 0.7522762884082468 1107 16 0.8969360370800601 1107 17 0.7501628909393148 1107 18 0.7406325262751636 1107 19 0.7803769803113241 1107 20 0.9616827571702302 1107 21 0.8830992888615867 1107 22 1.078147710339067 1107 23 0.9077902616188354 1107 24 1.1449062392222373 1107 25 1.4118840764638776 1108 1 0.6030006802524674 1108 2 2.2273479551287743 1108 3 1.2980746333514261 1108 4 0.7959331459335175 1108 5 0.4743287960478 1108 6 0.5547237795529796 1108 7 0.3433686249794121 1108 8 0.40961376521782 1108 9 0.2139756721723245 1108 10 0.3525948589138892 1108 11 0.31160111313700156 1108 12 0.36709342732427785 1108 13 0.18112295073122908 1108 14 0.3623609529338765 1108 15 0.34683477543455865 1108 16 0.28690549362585926 1108 17 0.5392079900786442 1108 18 0.6160263324443633 1108 19 0.53664048646705 1108 20 0.39032660208555114 1108 21 0.7266359948113035 1108 22 0.6444672071876028 1108 23 0.8881051982680717 1108 24 0.76712912319298 1108 25 0.6427866345204691 1109 1 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17 -1.5871439809026242 2315 18 -1.8654942281649909 2315 19 -1.8492013656689466 2315 20 -1.8527331202476134 2315 21 -1.9864839210750063 2315 22 -1.8755348733631507 2315 23 -1.6028911564336354 2315 24 -1.8218939901159175 2315 25 -1.878658208902607 2316 1 -1.1127855180276889 2316 2 -1.6887902651819893 2316 3 -0.8412533482078608 2316 4 -1.398501457796666 2316 5 -1.3057961888959078 2316 6 -1.3240394079882023 2316 7 -1.5169845543191869 2316 8 -1.520520312545866 2316 9 -1.7530368759192427 2316 10 -1.889300722447956 2316 11 -2.0486688137189333 2316 12 -2.016018371849576 2316 13 -1.9848464029227912 2316 14 -2.454354956666961 2316 15 -2.6795149178894277 2316 16 -2.2072314088164475 2316 17 -2.5035300351497938 2316 18 -2.234232635792175 2316 19 -2.4252729971221783 2316 20 -2.6974073018517615 2316 21 -2.6443557838623732 2316 22 -2.5280289017939808 2316 23 -2.457963397021139 2316 24 -2.3230917260527875 2316 25 -2.9144910460310527 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40940.0 3 2.0 4 344.0 5 1746.0 6 1980.0 7 1851.0 8 1757.0 9 1678.0 10 1683.0 11 1610.0 12 1603.0 13 1663.0 14 1594.0 15 1750.0 16 1828.0 17 1945.0 18 2342.0 19 2745.0 20 3536.0 21 4498.0 22 5760.0 23 7038.0 24 8166.0 25 9426.0 26 10652.0 27 12456.0 28 14551.0 29 17789.0 30 22363.0 31 28521.0 32 37740.0 33 49660.0 34 67809.0 35 93212.0 36 135583.0 37 186291.0 38 391050.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.741799003031076 18.489535910793574 13.180055175371566 24.58860991080379 2 17.09585554477738 21.476857084375307 37.516530654017636 23.910756716829674 3 18.43915987753178 24.123227265687625 30.119592022206298 27.3180208345743 4 12.96740364307219 16.815043372743503 36.9058904219163 33.31166256226801 5 14.058317066072593 36.94545943452903 34.8721282682728 14.12409523112558 6 32.25538266213509 36.090343288840174 17.721386498201948 13.93288755082278 7 28.138503457395664 32.13727128685237 21.583577413156654 18.14064784259532 8 26.220048708343903 34.42269039567446 20.601655266781147 18.7556056292005 9 26.496857454546692 15.3186539387761 20.088464398950954 38.09602420772625 10 15.79629021360459 28.372769030993172 33.268689763624074 22.562250991778154 11 33.83005917482016 23.065245472539107 23.582706043932667 19.521989308708076 12 22.818385890626143 25.079435856503192 30.55000076585186 21.552177487018813 13 28.309628800114368 21.608680335136775 25.963484183457258 24.1182066812916 14 22.067907406572218 21.37089534267278 27.534997391842296 29.02619985891271 15 24.04417433511295 28.38068283351572 23.999414548802633 23.5757282825687 16 23.146766147986405 27.966612262819936 26.00075564049893 22.885865948694732 17 22.413250258262266 27.65040054052122 27.054908174362343 22.881441026854173 18 22.114568034024245 27.18918753329328 28.22530000119132 22.470944431491148 19 23.268366403951116 27.056269688774826 27.97299436162844 21.702369545645624 20 23.537711003002993 26.447695251969733 28.05828307780806 21.956310667219213 21 23.647122694573174 26.200387691228954 27.726560252969378 22.425929361228494 22 23.527134223653327 26.148228578625172 27.687936430675702 22.63670076704579 23 23.517583578051994 26.060643708638576 27.476196838044892 22.94557587526454 24 22.97080995710375 26.27810146864983 27.72649875208588 23.02458982216054 25 23.25892089771453 26.313308292814096 27.607002268623386 22.820768540847986 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1715.0 1 1715.0 2 1273.5 3 832.0 4 832.0 5 832.0 6 1605.0 7 2378.0 8 2378.0 9 2378.0 10 3048.0 11 3718.0 12 3718.0 13 3718.0 14 5118.5 15 6519.0 16 6519.0 17 6519.0 18 10701.5 19 14884.0 20 14884.0 21 14884.0 22 21592.5 23 28301.0 24 28301.0 25 28301.0 26 38056.0 27 47811.0 28 47811.0 29 47811.0 30 59519.5 31 71228.0 32 71228.0 33 71228.0 34 82155.0 35 93082.0 36 93082.0 37 93082.0 38 104432.0 39 115782.0 40 115782.0 41 115782.0 42 127857.5 43 139933.0 44 139933.0 45 139933.0 46 145330.0 47 150727.0 48 150727.0 49 150727.0 50 151306.5 51 151886.0 52 151886.0 53 151886.0 54 140433.0 55 128980.0 56 128980.0 57 128980.0 58 112191.5 59 95403.0 60 95403.0 61 95403.0 62 78682.0 63 61961.0 64 61961.0 65 61961.0 66 48092.5 67 34224.0 68 34224.0 69 34224.0 70 25141.0 71 16058.0 72 16058.0 73 16058.0 74 11243.5 75 6429.0 76 6429.0 77 6429.0 78 4360.5 79 2292.0 80 2292.0 81 2292.0 82 1508.0 83 724.0 84 724.0 85 724.0 86 466.5 87 209.0 88 209.0 89 209.0 90 137.0 91 65.0 92 65.0 93 65.0 94 40.0 95 15.0 96 15.0 97 15.0 98 10.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.381693757966987 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.403786031202507E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.5528395234018797E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 8.509465078006267E-5 21 0.0 22 3.403786031202507E-4 23 2.5528395234018797E-4 24 8.509465078006267E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1175162.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.31830468129917 #Duplication Level Percentage of deduplicated Percentage of total 1 81.15940008936559 58.693102234142 2 12.661335270821978 18.312926035747665 3 3.4013656161402497 7.379429848815764 4 1.235153471703584 3.5729682037929686 5 0.5516593660340933 1.994753505657295 6 0.30128787567279297 1.3073177033811858 7 0.18218492783087123 0.9222713583449411 8 0.1047488936770371 0.6060209926371984 9 0.07603865123621031 0.49490877128897737 >10 0.30994225996172475 3.650490452716959 >50 0.010114961302389237 0.5195035812920603 >100 0.004987410773357622 0.6878682498636912 >500 0.0011874703201181043 0.6346224606321239 >1k 5.937351600590521E-4 1.2238166016871987 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4747 0.40394430725295744 No Hit GGTATCAACGCAGAGTACTTTTTTT 3387 0.2882155821920722 No Hit TATCAACGCAGAGTACTTTTTTTTT 3196 0.2719625038930803 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1535 0.1306202889473962 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1386 0.11794118598116685 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.509465078006266E-5 2 0.0 0.0 0.0 0.0 8.509465078006266E-5 3 0.0 0.0 0.0 8.509465078006266E-5 8.509465078006266E-5 4 0.0 0.0 0.0 8.509465078006266E-5 8.509465078006266E-5 5 0.0 0.0 0.0 8.509465078006266E-5 8.509465078006266E-5 6 0.0 0.0 0.0 8.509465078006266E-5 2.5528395234018797E-4 7 0.0 0.0 0.0 8.509465078006266E-5 2.5528395234018797E-4 8 0.0 0.0 0.0 8.509465078006266E-5 2.5528395234018797E-4 9 0.0 0.0 0.0 1.7018930156012532E-4 2.5528395234018797E-4 10 0.0 0.0 0.0 1.7018930156012532E-4 2.5528395234018797E-4 11 0.0 0.0 0.0 1.7018930156012532E-4 2.5528395234018797E-4 12 0.0 0.0 0.0 1.7018930156012532E-4 3.4037860312025064E-4 13 0.0 0.0 0.0 2.5528395234018797E-4 3.4037860312025064E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGAGCCG 50 0.0015523126 13.2386465 5 GGTATCA 1250 0.0 11.630144 1 TAATACC 100 2.0383177E-6 11.347411 4 CTATACT 100 2.0383177E-6 11.347411 4 CTAGGAC 170 1.2732926E-11 11.124912 3 TGTACCG 60 0.0060798526 11.032205 5 CCCTAGG 100 1.5370491E-5 10.878537 1 TAAGGCT 115 9.347914E-7 10.6895895 4 ATAATAC 125 2.3844223E-7 10.590918 3 CTCGAAC 75 0.0027502147 10.086588 18 TAGGACC 75 0.0027502147 10.086588 4 CCTAGGA 170 8.609277E-9 9.889579 2 GTACATA 290 0.0 9.548559 1 TCTATAC 110 7.185167E-5 9.456176 3 GCTATAC 80 0.0046895174 9.456175 3 TTATACT 160 7.111885E-8 9.456175 4 TAGATTG 80 0.0046895174 9.456175 5 GGACTAG 95 0.001263522 9.369074 1 GTTATAA 85 0.005341689 9.307838 1 GTATATA 205 2.0027073E-9 9.165951 1 >>END_MODULE