FastQCFastQC Report
Thu 26 May 2016
SRR1512091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175162
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59360.5051218470304519No Hit
GGTATCAACGCAGAGTACTTTTTTT43240.367949269972991No Hit
TATCAACGCAGAGTACTTTTTTTTT43040.36624737695738974No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19770.1682321245921839No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15410.13113085685207657No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12710.10815530114145966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATG701.0927921E-412.2114958
GGTATCA13350.012.0712381
GGGTATC657.7732914E-411.7360771
TGGACCG600.00587489711.083165
AAGCGTG600.00588420911.0808027
CGTGCGC600.00588607311.08033110
GTATTAT803.6412457E-410.7275091
GTCCTAC750.002565947410.17126751
CTTATAC750.002647093310.1331753
GTATAGA1159.809011E-69.9501521
GTATAAT1252.447261E-69.91698551
ACTGTCC2500.09.8777438
CTAGACT1451.614917E-79.8274324
GTCCTGA2500.09.5355631
TTAGGAC900.00111324729.4998513
TAAACTA1002.7539846E-49.4998515
CTAAGAC1304.234347E-69.4998513
TATTCTG1804.1727617E-99.4998515
CATAACA1201.6990301E-59.4998514
ACTATGC800.00452257939.497838
Produced by FastQC (version 0.11.5)