FastQCFastQC Report
Thu 26 May 2016
SRR1512090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199630
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58310.2650900378700054No Hit
TATCAACGCAGAGTACTTTTTTTTT39620.18012120220218855No Hit
GGTATCAACGCAGAGTACTTTTTTT38440.17475666362069983No Hit
GTCCTAAAGTGTGTATTTCTCATTT33910.15416229093074746No Hit
CTGTAGGACGTGGAATATGGCAAGA31540.1433877515764015No Hit
GTCCTACAGTGGACATTTCTAAATT31530.14334228938503293No Hit
CTTTAGGACGTGAAATATGGCGAGG30970.14079640666839421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5350.012.1953444
TGGACCG550.00317818112.0346685
GGTATCA14050.011.618091
GTATACG600.00434438811.541811
AGGACCG600.00608445611.0317795
GTCCTAA10950.010.7512741
TCCAACG1855.456968E-1210.73362318
AGGACGG1159.366631E-710.68917755
CCAACGT1159.366631E-710.689177519
GGCGAGG9700.010.52811919
GTATAAG2550.010.0869591
GTATAGG1905.0386006E-109.892981
CGGTAGG1001.8329309E-49.892981
CTCGAAC1857.057679E-109.71137318
CCTAGAC2350.09.6569983
TGGCGAG19650.09.57611318
GTCCTAC15600.09.5758971
GGTCTAT1459.830692E-79.5518421
AATGTCC5650.09.5394918
GTCCAAC1354.091269E-69.5265721
Produced by FastQC (version 0.11.5)