FastQCFastQC Report
Thu 26 May 2016
SRR1512090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199630
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70810.32191777708069086No Hit
TATCAACGCAGAGTACTTTTTTTTT50900.23140255406591106No Hit
GGTATCAACGCAGAGTACTTTTTTT50020.22740188122547883No Hit
CTTTAGGACGTGAAATATGGCGAGG35820.16284556948214018No Hit
GTCCTAAAGTGTGTATTTCTCATTT35740.1624818719511918No Hit
GTCCTACAGTGGACATTTCTAAATT34740.15793565281433697No Hit
CTGTAGGACGTGGAATATGGCAAGA34380.1562990139250692No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24750.11251892363715715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG9150.011.93606219
GCGCCAC1201.0075382E-811.87065213
GTGCGCC1052.734614E-711.75759911
GGTATCA15100.011.6863471
TAGACGA752.0697933E-411.4028245
AGGACCG855.311535E-511.179245
GGACGTA600.00589301311.0792766
TGCGCCA1408.403731E-910.85316812
TAGGACC5100.010.8065994
CGTGCGC1106.074224E-610.35984210
TGTACGG750.002642881410.1358435
GAGGCGT750.002656375810.1296236
TTAGGAC13800.010.0532153
AGGACGT20850.09.980895
GTCTAGG2201.8189894E-129.9721591
GTATAAA3350.09.9656871
TGTAGGA19700.09.8882372
GGCGTGC1252.5828704E-69.8763838
GGACGTG20350.09.799856
TGGCGAG19500.09.64306118
Produced by FastQC (version 0.11.5)